Protocol for purifying recombinant HBV capsids from HepG2 cells and comparative phosphorylation analysis by phosphate-affinity SDS-PAGE [0.03%]
从HepG2细胞中纯化重组乙肝病毒衣壳的方案及磷酸选择性SDS-PAGE比较分析法
Katie Culhane,Shiv Kumar,Jianming Hu et al.
Katie Culhane et al.
The hepatitis B virus (HBV) capsid protein (HBc or Cp) contains multiple phosphorylation sites that regulate distinct stages of the viral lifecycle. Here, we present a protocol for purifying HBV capsids by gradient ultracentrifugation and a...
Protocol for quantifying vertebral column morphology for the statistical analysis of scoliosis severity in zebrafish [0.03%]
用于统计分析斑马鱼脊柱侧凸严重程度的量化椎柱形态的协议
Brittney Voigt,Ece Atayeter,Ron Sistrunk et al.
Brittney Voigt et al.
Here, we present a protocol for quantifying zebrafish vertebral column morphology using micro-computed tomography (microCT) datasets for scoliosis researchers. We describe steps for orientation of skeletal and cranial microCT datasets in Da...
Protocol for KAT8 conditional knockout mice generation and its application in 4NQO-induced esophageal tumor model [0.03%]
KAT8 条件性敲除小鼠制备方案及其在建立 4NQO 致食道肿瘤模型中的应用
Dandan Zhang,Ming Jiang,Kangdong Liu et al.
Dandan Zhang et al.
4-Nitroquinoline 1-oxide (4NQO), a quinoline derivative, induces tumor formation in mice following a multi-stage pattern analogous to human tumors, mimicking the natural carcinogenesis process. Here, we present a protocol for inducing esoph...
Protocol for generating an in vitro 3D multicellular culture model of ovarian high-grade serous carcinoma [0.03%]
卵巢高级别浆液性癌的体外三维多细胞培养模型制备方案
Krister Wennerberg,Daria Bulanova,Laura Gall-Mas et al.
Krister Wennerberg et al.
The development of translational ovarian cancer models to investigate and overcome treatment resistance, while accounting for the impact of the tumor microenvironment, is critical. Here, we present a protocol to establish a multicellular cu...
Protocol for analyzing immune cell infiltration in 3D pancreatic heterotypic spheroids composed of tumor cells and cancer-associated fibroblasts [0.03%]
三维胰腺异质性球体中肿瘤细胞和癌症相关成纤维细胞浸润免疫细胞的分析协议
Bessede Thomas,Vezzio-Vie Nadia,Freixinos Clara et al.
Bessede Thomas et al.
We present a protocol to generate and analyze 3D cancer models composed of tumor cells, cancer-associated fibroblasts (CAFs), and immune cells that mimic the complexity of the pancreatic ductal adenocarcinoma (PDAC) stroma. The protocol inc...
Protocol for chromatin immunoprecipitation using isolated antheridia of Marchantia polymorpha [0.03%]
地钱雄器细胞的染色质免疫沉淀实验手册
Keisuke Inoue,Kanta Kotani,Shohei Yamaoka et al.
Keisuke Inoue et al.
Chromatin immunoprecipitation (ChIP) is a fundamental technique used to investigate the binding sites of transcription factors (TFs) and histone modifications throughout the genome. Here, we present the ChIP protocol using the model bryophy...
Protocol for generating human tonsil immune organoids with epithelial and germinal center layers using low-Matrigel culture [0.03%]
用于生成具有上皮层和生发中心层的人扁桃体免疫类器官的低基质胶培养协议
Dandan Meng,Han Ding,Qiyu Deng et al.
Dandan Meng et al.
Tonsil immune organoids traditionally include either epithelial layers or immune compartments, failing to recapitulate the integrated physiological microenvironment of human tonsils. Here, we present a protocol to generate tonsil immune org...
Protocol for assessing visual stimulus discrimination in Danionella cerebrum and Danio rerio using habituation/dishabituation [0.03%]
用于斑马鱼和小头鱼视觉刺激辨别能力的习得/去习得实验评估方案
Mirko Zanon,Davide Potrich,Andrea Messina et al.
Mirko Zanon et al.
Here, we present a protocol to assess visual discrimination in fish, specifically Danionella cerebrum and Danio rerio. We describe steps for the familiarization of subjects to reduce contextual novelty and habituation through repeated prese...
Protocol for spatial sampling and monitoring infection disease dynamics and pathogen spread in social ambrosia beetle colonies [0.03%]
用于监测社会木蚁菌落感染病动态和病原体传播的空间采样协议
Abolfazl Masoudi,Ross A Joseph,Nemat O Keyhani
Abolfazl Masoudi
Here, we present a protocol for investigating spatial sampling, infection, and pathogen spread in ambrosia beetle colonies to explore infectious disease dynamics in social organisms. We describe steps for rearing Xyleborus affinis colonies,...
Protocol for combining BMP, MEK, WNT inhibition and iNGN2 to enable rapid neuronal differentiation and regional patterning from hiPSCs [0.03%]
结合BMP、MEK、Wnt抑制和iNGN2使hiPSC快速向神经元分化并进行区域模式构建的方案
Carina Habich,Astrid Doering,Justine D Manos et al.
Carina Habich et al.
Neurogenin 2 (iNGN2) overexpression induces human induced pluripotent stem cells (hiPSCs) into neurons but results in a mixed population of peripheral and central nervous system neurons. Pre-differentiation using BMP, MEK, and WNT inhibitio...