Covalent anchoring of tethered ligands to chemogenetic handles for targeted in vivo neuropharmacology [0.03%]
用于靶向体内神经药理学的连接配体的共价固定化化学遗传受体
Maria Ciscato,Kian Stenbolt,Alexandre Mourot et al.
Maria Ciscato et al.
Covalent anchoring of tethered ligands to chemogenetic handles (CATCH) is a chemogenetic approach that enables sustained, localized, and pharmacologically specific receptor antagonism. Here, we present a protocol for designing and applying ...
Protocol to quantify mechanical properties of cells using optical tweezers [0.03%]
基于光学镊技术定量测量细胞机械性质的协议书
Wessel S Rodenburg,Jorine M Eeftens
Wessel S Rodenburg
Mechanical properties of cells determine their ability to deform when subjected to force, which is crucial in many biological processes. Here, we present a protocol to quantify these properties on living cells by applying piconewton-range f...
Protocol for the generation of three-dimensional micropatterned neuroepithelial tissues using hPSCs, bioprinting, and matrix scaffolds [0.03%]
基于人多能干细胞、生物打印和基质支架的三维微模式神经上皮组织构建方案
Kenneth Kin Lam Wong,Negin Imani Farahani,George Allen et al.
Kenneth Kin Lam Wong et al.
Micropatterning technology that spatially guides the self-assembly of human pluripotent stem cells (hPSCs) into neural tissues offers enhanced fidelity to investigate early neurodevelopment and disease mechanisms. Here, we present a protoco...
Protocol to investigate cell cycle-dependent modes of micronucleus formation using dual-pulse nascent DNA labeling [0.03%]
采用新生DNA双脉冲标记法研究细胞周期依赖性微核形成机制
Simona Pepe,Renée Concetta Duardo,Giovanni Capranico et al.
Simona Pepe et al.
Here, we present a protocol to study the contribution of individual cell-cycle stages to genome instability mechanisms using dual-pulse nascent DNA labeling. We describe steps for sample treatment, immunofluorescence staining, imaging, and ...
Protocol for isolating nuclei from human stem cell-derived grafts for single-nucleus RNA sequencing [0.03%]
人干细胞衍生移植物单细胞核RNA测序的核分离方案
Edoardo Sozzi,Petter Storm,Alessandro Fiorenzano
Edoardo Sozzi
Single-nucleus RNA sequencing enables high-resolution transcriptomic profiling of brain tissue, facilitating analysis of cell identity in models of neurodegeneration and repair. Here, we present a protocol for isolating nuclei from long-ter...
Protocol for cell-specific RNA expression profiling and lipidomics analyses of Drosophila melanogaster intestinal progenitor cells [0.03%]
果蝇肠道干细胞RNA表达和脂质组分析的细胞特异性协议
Stephanie Makdissi,Ahsan Malick,Arash Mohammadi Matak et al.
Stephanie Makdissi et al.
Research in the model system Drosophila melanogaster has highlighted the importance of cell-specific in vivo omics analyses to study intestinal epithelial homeostasis. Here, we present an optimized protocol for profiling intestinal progenit...
Protocol for an automated virtual screening pipeline including library generation and docking evaluation [0.03%]
包括文库生成和对接评估的自动化虚拟筛选流程协议
Pedro José Barbosa Pereira,Jorge Ripoll-Rozada,Sandra Macedo-Ribeiro et al.
Pedro José Barbosa Pereira et al.
Here, we present a protocol for an automated virtual screening pipeline. We describe steps for generating compound libraries for computational docking including Food and Drug Administration (FDA)-approved drugs, setting up the receptor and ...
Yue Tian,Ruihan Jiang,Fang Guo
Yue Tian
Two-photon calcium imaging facilitates the real-time observation of neuronal activity. Here, we present a protocol for conducting in vivo two-photon calcium imaging of the Drosophila melanogaster brain. We describe steps for fly preparation...
Protocol for the quantification of mouse fecal IgM, IgG, and IgA utilizing standard curve-based enzyme-linked immunosorbent assays [0.03%]
基于标准曲线的酶联免疫吸附测定法量化小鼠粪便IgM、IgG和IgA的方法学protocol
KimAnh T Pioli,Peter D Pioli
KimAnh T Pioli
Immunoglobulin (Ig) transport into the intestinal lumen is of interest as Igs modulate gut microbial species composition and play protective roles upon exposure to intestinal pathogens. Here, we present a protocol for the quantification of ...
Scanning probe microscopy for rheological analysis of biomolecular condensates [0.03%]
用于生物分子凝聚体流变学分析的扫描探针显微镜技术
Aida Naghilou,Alireza Mashaghi
Aida Naghilou
Biomolecular condensates are membrane-less phase-separated assemblies that play key roles in cellular organization. Here, we present a protocol to characterize the full range of rheological properties of condensate droplets, from liquid to ...