Protocol for the generation, characterization, and functional assays of organoid cultures from normal and cancer-prone human esophageal tissues [0.03%]
人正常和易患癌症的食道组织类器官培养的产生、表征和功能测定协议
Shaosen Zhang,Guoyu Cheng,Shihao Zhu et al.
Shaosen Zhang et al.
Published Erratum
STAR protocols. 2026 Jul 23;7(3):104763. DOI:10.1016/j.xpro.2026.104763 2026
Protocol for studying Chemerin 15-enhanced microglial phagocytosis in cerebral ischemia-reperfusion injury via the ChemR23/p38 MAPK pathway [0.03%]
通过Chemr23/p38MAPK信号通路研究后环素15增强小胶质细胞吞噬作用在脑缺血再灌注损伤中的机制探讨规范
Changhao Mao,Jun Li,Yixuan Zhou et al.
Changhao Mao et al.
Chemerin 15 (C15), a chemerin-derived peptide, enhances microglial phagocytosis through the ChemR23/p38 MAPK pathway to alleviate brain damage after cerebral ischemia-reperfusion (I/R) injury. Here, we present a protocol for studying C15-en...
Protocol for in vivo DNA-RNA hybrid immunoprecipitation, sequencing, and analysis from frozen mouse tissues [0.03%]
小鼠冻存组织的体内DNA-RNA杂交免疫沉淀、测序及分析协议
Hassan Massalha,Cameron J Chee,Julia S P Mawer et al.
Hassan Massalha et al.
DNA-RNA hybrids (R-loops) form transiently on the genome and regulate cellular homeostasis. They also influence genome-editing outcomes, highlighting their therapeutic potential. Here, we present a protocol for high-resolution mapping of DN...
Protocol for luminescent nanocrystal synthesis via modified ligand-assisted precipitation and X-ray scintillation film fabrication [0.03%]
改良型配体辅助沉淀法合成发光纳米晶及制备X射线闪烁薄膜的方法_protocol
Azimet A Karluk,Javeed Mahmood,Cafer T Yavuz
Azimet A Karluk
The field of all-inorganic metal halide nanocrystals currently faces the challenge of developing efficient approaches for the scalable, facile, and stable synthesis of nanocrystals (NCs). Here, we present a protocol for the acid-mediated mo...
Protocol for SEC-based isolation and characterization of human plasma-derived extracellular vesicles [0.03%]
基于SEC的人类血浆源外泌体分离和特征分析的协议
Fanni Annamária Boros,Philipp Arnold,Martin Regensburger et al.
Fanni Annamária Boros et al.
Extracellular vesicles (EVs) are gaining increasing attention among studies focusing on biomarker research, elucidating disease mechanisms, and exploring therapeutic approaches. Here, we present a method for size-exclusion chromatography (S...
Protocol for generation of a 3D humanized lung-tumor-on-a-chip model of immune-hot and immune-cold microenvironments [0.03%]
构建免疫激活和非激活肿瘤微环境的三维人源化肺肿瘤芯片模型协议
Sabrina Brito Añez,Sanne van Kesteren,Peter Carmeliet et al.
Sabrina Brito Añez et al.
The tumor microenvironment (TME) modulates immune responses and clinical outcomes. Here, we present a protocol to establish a fully humanized immune-hot and -cold lung TME using the Emulate organ-on-a-chip system by culturing human lung mic...
A 3D RNA-protein proximity ligation protocol detecting the heterogeneity of LISRR-RPL26 interactions in patient-derived melanoma tumor explants [0.03%]
用于检测患者来源黑色素瘤肿瘤组织中LISRR-RPL26相互作用异质性的三维RNA-蛋白质邻近连接协议
Alessandro Marino,Eleonora Leucci
Alessandro Marino
Investigating lncRNA-protein interactors is crucial for understanding their contribution to tumor formation and progression. Here, we present a protocol to explore the heterogeneity of RNA-protein interactions between the lncRNA LISRR and R...
Protocol for classifying cellular senescence from single-cell and single-nucleus RNA sequencing data [0.03%]
基于单细胞和单核RNA测序数据分类细胞衰老的协议
Anina N Lund,Ryan C Thompson,Brian H Kopell et al.
Anina N Lund et al.
Cellular senescence is a cell state characterized by stable cell-cycle arrest accompanied by coordinated molecular, metabolic, and functional changes. Here, we present a protocol to classify senescent cells from single-cell or single-nucleu...
Protocol for isolation of primary hepatic lymphatic endothelial cells from rat liver [0.03%]
大鼠肝脏初级肝淋巴内皮细胞分离方案
Aarti Sharma,Deepika Jakhar,Pinky Juneja et al.
Aarti Sharma et al.
Here, we present a protocol for isolating and purifying primary rat hepatic lymphatic endothelial cells (hLECs) from an enriched population of hepatic endothelial cells (hECs). We describe procedures for rat liver perfusion, portal vascular...
Protocol for quantifying Drosophila feeding behavior using flyPAD and optoPAD [0.03%]
使用flyPAD和optoPAD量化果蝇摄食行为的协议
Nicholas J Collins,Madison N Endres,Irina T Sinakevitch et al.
Nicholas J Collins et al.
Quantifying feeding behavior with precision is essential for understanding how internal state, sensory cues, and neural activity influence food intake and dietary choice. Here, we present a protocol for performing consumption and dietary ch...