Protocol for preparing and characterizing samples for combined microsecond freeze-hyperquenching and electron paramagnetic resonance spectroscopy [0.03%]
纳秒冻滞技术与电子顺磁共振联用样品制备和表征的方案
Joshua L Wort,Tobias Hett,Adrian Haardt et al.
Joshua L Wort et al.
Microsecond freeze-hyperquenching (MHQ) coupled with pulsed electron-electron double resonance spectroscopy (PELDOR) can resolve conformational changes in biomolecules over space and time. Here, we present a protocol for preparing MHQ sampl...
Protocol for the preparation of flexible material from the mycelium of wood-decay fungal strains and mechanical property investigation [0.03%]
基于木腐菌菌丝体制备柔性材料的工艺及其力学性能研究
Dhanalakshmi Vadivel,Daniele Dondi
Dhanalakshmi Vadivel
Extraction of chitin-glucan-based materials from fungi has several advantages over animal-based chitin. Here, we present a protocol for extracting chitin-glucan material from the mycelium of wood-decay fungal strains and investigating its m...
Protocol for formulation and evaluation of phytosterol-based lipid nanoparticles as cholesterol alternatives for mRNA delivery [0.03%]
基于植物甾醇的脂质纳米颗粒作为mRNA递送用胆固醇替代品的制备和评价方案
Jaewook Lee,Jae Hoon Kim,Seung-Yeul Lee et al.
Jaewook Lee et al.
We present a protocol for formulating and evaluating lipid nanoparticles (LNPs) that incorporate the plant-derived sterols ginsenoside Rg2 and protopanaxadiol (PPD) as substitutes for cholesterol. The protocol describes mRNA preparation, ma...
Yabi Huang,Zijian Li
Yabi Huang
Pesticide residues in the environment threaten agricultural and human health. Here, we present a framework for mapping and comparing the contamination levels of pesticides across specific environmental compartments in various regions. We de...
Protocol for efficient CRISPRi-mediated silencing of retrotransposons in human pluripotent stem cells [0.03%]
有效利用CRISPRi介导的方法在人类多能干细胞中沉默逆转座子的方案
Anita Adami,Raquel Garza,Fereshteh Dorazehi et al.
Anita Adami et al.
Here, we present a workflow for transcriptional silencing of transposable elements (TEs) in human induced pluripotent stem cells (hiPSCs). We describe steps for designing guide RNAs (gRNAs) to target TE families or unique TE loci. We also d...
Protocol for evaluating seedling greening capacity during dark-to-light transition [0.03%]
从暗到光转变过程中评价幼苗绿化能力的方案
Yixuan Fan,Shangwei Zhong
Yixuan Fan
We present a protocol to evaluate greening capacity in etiolated Arabidopsis seedlings during the critical dark-to-light transition. We describe steps for sample preparation and sowing and then detail procedures for quantifying protochlorop...
Computational protocol for hierarchical Bayesian modeling of perception and generalization in fear conditioning [0.03%]
恐惧条件作用中的感知和泛化的分层贝叶斯模型的计算协议
Kenny Yu,Wolf Vanpaemel,Francis Tuerlinckx et al.
Kenny Yu et al.
Understanding human generalization behavior requires disentangling underlying cognitive and perceptual mechanisms. Here, we present a computational protocol to analyze individual differences in fear generalization by integrating a Bayesian ...
Protocol for the formulation and characterization of extracellular vesicle-crosslinked supramolecular hydrogels [0.03%]
用于制备和表征细胞外囊泡交联的超分子水凝胶的方案
Artemis Margaronis,James S Wang,Yara A Aguilar et al.
Artemis Margaronis et al.
Extracellular vesicles (EVs) can serve as supramolecular crosslinkers to form injectable, intrinsically bioactive hydrogels. This protocol details the formulation, rheological characterization, and analysis of EV-crosslinked hydrogels with ...
Protocol for the generation of a human-derived nasal epithelial model and induction of tissue-resident memory-like T cells in a co-culture system [0.03%]
人源鼻上皮模型的构建及共培养体系中组织驻留记忆样T细胞诱导的方法学探索
Lisa A King,Iris van der Valk,Jasmijn S Schrumpf et al.
Lisa A King et al.
Tissue-resident memory T cells (TRMs) are a distinct subset of T lymphocytes that permanently localize in non-lymphoid tissues and mediate rapid, localized immune responses. Here, we present a protocol to study human TRMs using an in vitro ...
Protocol for automated analysis of biological images using Python code [0.03%]
基于Python代码进行生物图像自动化分析的协议
Benjamin Sacks,Jiya Mody,Yiming Huang et al.
Benjamin Sacks et al.
Image analysis in Python provides greater flexibility and an open-source alternative to commercially available software. In this protocol, we present steps for quantifying features in various common biological images in a manner that is tai...