Bridging mesoscopic and microscopic scales in multiple sclerosis: Post mortem brain block multi-contrast 9.4T MRI and histology quantification [0.03%]
桥接多发性硬化的介观和微观尺度：死后脑块多对比9.4T磁共振成像和组织学定量分析
Dimitrios G Gkotsoulias,Lukas Schönenberger,Jochen Leupold et al.
Dimitrios G Gkotsoulias et al.
Combining ex vivo MRI mesoscopic and histopathology microscopic assessments of tissue microstructure forms a key approach in multiple sclerosis (MS) research for elucidating disease pathogenesis. We present a protocol that enables reproduci...
Protocol for quantifying the thickness and puncture resistance properties of solitary bee cocoons using Osmia lignaria as a model [0.03%]
一种使用Osmia lignaria作为模型量化独栖蜜蜂巢孔厚度和抗穿刺性能的协议
Oran Wasserman,Mallory R Wootton,Spencer Fairbanks et al.
Oran Wasserman et al.
Solitary bee cocoons serve as protective barriers against parasitoids, yet their puncture resistance properties remain understudied. Here, we present a protocol for measuring puncture resistance in Osmia lignaria cocoons using custom 3D-pri...
Protocol for genetic discovery and fine-mapping of multivariate latent factors from high-dimensional traits [0.03%]
高维性状多变量潜在因素的遗传发现和精细定位协议
William J Astle,Adam S Butterworth,Jennifer L Asimit
William J Astle
High-dimensional traits, like blood cell traits, are often analyzed using univariate genetic analysis approaches, ignoring trait relationships. Here, we present a protocol for using the flashfmZero software for analyses of latent factors th...
Protocol to generate stable knockout lines in the human-parasitic nematode Strongyloides stercoralis [0.03%]
用于人体寄生线虫斯氏鞭虫（Strongyloides stercoralis）的稳定基因敲除系生成协议
Navonil Banerjee,Breanna Walsh,Ruhi Patel et al.
Navonil Banerjee et al.
A major limitation to the study of gene function in parasitic nematodes was the inability to make stable mutant lines. Here, we present a protocol for generating stable knockout lines in the human-parasitic nematode Strongyloides stercorali...
Protocol for culturing olfactory epithelium organoids supporting neuronal differentiation [0.03%]
支持神经元分化的嗅觉上皮类器官培养体系构建及优化项目
Juliana Gutschow Gameiro,Jussi Virtanen,Liam Lee et al.
Juliana Gutschow Gameiro et al.
The olfactory epithelium (OE) contains stem cells capable of generating olfactory sensory neurons throughout life, making it a valuable model for studying epithelial neurogenesis. We present a protocol to develop a highly robust 3D mouse or...
Protocol for dynamic high-throughput cell death screening of primary phagocytes following microplastic and nanoplastic exposure [0.03%]
原代吞噬细胞暴露于微塑料和纳米塑料后的动态高通量细胞死亡筛选协议
Tim Leonardus Philip Skrabanja,Joëlle Anna Zoetje Klazen,Sarah Sayed et al.
Tim Leonardus Philip Skrabanja et al.
Micro- and nanoplastics (MNPs) can cross epithelial barriers of the lung and/or intestine into the bloodstream. In the body, phagocytes will be exposed to plastic particles, but they are incapable of degrading them. Here, we present a proto...
FlowLITE: A protocol to characterize and quantify total antibody isotypes in human plasma using flow cytometry [0.03%]
一种使用流式细胞术表征并定量人血浆中总抗体亚型的协议(FlowLITE)
Hyunjae J Hahm,Alexandra F Nazzari,Benjamin R Babcock et al.
Hyunjae J Hahm et al.
FlowLITE is a flow cytometry bead-based assay that enables multiplexing and high-throughput quantification of all antibody isotypes from humans, mice, or other species, using small volumes of any fluid specimen. Here, we detail a FlowLITE p...
Protocol for preparation of mouse synovium for flow cytometry and RNA-seq [0.03%]
小鼠滑膜(flow cytometry和RNA-seq)的制备协议
Anna B Montgomery,Carla M Cuda,Salina Dominguez et al.
Anna B Montgomery et al.
The mouse synovium can be used to model the sterile inflammation observed in rheumatoid arthritis and osteoarthritis. Here, we present a protocol for inducing arthritis and harvesting and preparing a single-cell suspension from cells of int...
Protocol for autofluorescence removal in microglia by photobleaching in free-floating immunofluorescent staining of mouse brain tissue [0.03%]
小鼠脑组织自由漂浮式免疫荧光染色中通过光漂白去除小胶质细胞自发荧光的协议
Mark N Metri,Justin You,Jeehye Park
Mark N Metri
Autofluorescence in brain tissue poses a challenge in immunofluorescent staining by obscuring antibody-labeled proteins, mainly within microglia. Here, we describe a cost-effective protocol for removing autofluorescence in mouse brain secti...
Protocol for creating a gene dictionary for organelle genomes using the Gene Dictionary Tool [0.03%]
利用基因词典工具创建细胞器基因组的基因词典的协议
Breno Dupin,Matheus Sanita Lima,Alexandre Rossi Paschoal et al.
Breno Dupin et al.
Here, we present a protocol for creating a gene dictionary for fungal mitochondrial genomes using the Gene Dictionary Tool. Through a Python Command Line Interface (CLI), the user identifies what annotations are missing in the inputted dict...