Protocol to build open-source Discrimin8 maze to study discrimination of reward-context associations in mice during open-field foraging [0.03%]
构建开放式 Discrimin8 迷宫以在旷场觅食期间研究小鼠对奖励-情境关联的辨别能力
Gergely Tarcsay,Laura A Ewell
Gergely Tarcsay
The hippocampus is known to process context-specific memories. We provide a protocol for studying context discrimination in mice utilizing the open-source Discrimin8 maze. We describe the steps for building the maze and using custom-written...
Protocol for generating a 3D hydrogel-based tumor resection model in vitro using pancreatic cancer cells [0.03%]
基于胰腺癌细胞的体外三维凝胶肿瘤切除模型制备方法学研究报告
Lea Miebach,Marten Hagge,Linus Hübner et al.
Lea Miebach et al.
We present a protocol for the generation of two distinct in vitro tumor resection models to evaluate cellular responses at tumor margins in 3D. We describe steps for patterning pancreatic cancer cells (Panc-01) embedded in hydrogels using a...
Da-Wei Lin,Cara Teixeira,Carolina Chung et al.
Da-Wei Lin et al.
Single-cell optimization objective and trade-off inference (SCOOTI) is a computational framework that integrates bulk and single-cell omics data with genome-scale metabolic modeling to infer metabolic objectives and trade-offs in biological...
Protocol to fabricate elastomer microwells for three-dimensional culture of primary adipocytes [0.03%]
用于原代脂肪细胞三维培养的弹性微井制造方案
Garrett F Beeghly,Jenny Deng,Claudia Fischbach
Garrett F Beeghly
Published Erratum
STAR protocols. 2026 Mar 4;7(1):104437. DOI:10.1016/j.xpro.2026.104437 2026
Protocol for mouse vascular dementia model and evaluation of progressive tissue damage in subcortical white matter and adjacent cortex [0.03%]
小鼠血管性痴呆模型制备及皮层下白质及其邻近皮层进行性损伤评价方法学研究
Min Tian,Irene L Llorente,S Thomas Carmichael
Min Tian
Here, we present a protocol for generating a vascular dementia (VaD) mouse model through stereotaxic microinjection of a vasoconstrictor into the subcortical white matter (WM). We describe steps for preparing the N5-(1-iminoethyl)-L-ornithi...
Protocol for MR1-ligand cross-linking to discover metabolite antigens by proteomics [0.03%]
基于蛋白质组学的MR1配体交联技术发现代谢物抗原的方案
Hannah Thomas,Garry Dolton,Lucia F Cardo et al.
Hannah Thomas et al.
Here, we present a protocol for the discovery of MR1-bound metabolite ligands by stabilizing Schiff base interactions between MR1 and its ligands. We describe steps for enriching MR1-ligand complexes using MR1-expressing A549 cells and cova...
Protocol for the precise activation of intradental neurons in mice via electrical stimulation [0.03%]
通过电刺激在小鼠中精确激活牙本质神经元的方案
Shuhao Wan,Elizabeth A Ronan,Aditi Jha et al.
Shuhao Wan et al.
Intradental neurons encode tooth sensation, yet identifying them without sacrificing tooth structural integrity remains challenging. Here, we present a protocol for the non-invasive electrical stimulation of intradental neurons with single-...
Protocol for establishing a chemoresistant orthotopic triple-negative breast cancer patient-derived xenograft model for preclinical drug testing [0.03%]
用于药物检测的三阴性乳腺癌类器官异种移植模型构建方案
David Tébar-García,Rosa Barbella-Aponte,Esther Sánchez-López et al.
David Tébar-García et al.
Here, we present a protocol for establishing patient-derived xenograft (PDX) mouse models of chemoresistant triple-negative breast cancer (TNBC). We describe steps for tumor collection, surgical implantation, and serial expansion. We then d...
Protocol to investigate the effects of localized gene expression disruption on mouse limb development via direct siRNA injection and explant culture [0.03%]
直接注射siRNA和组织切片培养检测基因表达干扰对鼠肢发育影响的方法学研究.protocol
Ji-Hye Yea,Sharon E S Onggo,Suhani Dewagan et al.
Ji-Hye Yea et al.
Here, we present a protocol for localized gene knockdown in embryonic mouse hindlimb explants using direct small interfering RNA (siRNA) injection, combined with a semi-quantitative morphology scoring system to assess developmental outcomes...
Protocol for generating megakaryocytes from patient induced pluripotent stem cells for disease modeling and compound screening [0.03%]
用于疾病建模和化合物筛选的诱导多能干细胞生成巨核细胞的方案
Payal Chawla,Niclas Flosdorf,Marcelo A S de Toledo et al.
Payal Chawla et al.
Here, we present a protocol for generating megakaryocytes from patient induced pluripotent stem cells (iPSCs). We describe steps for expanding and dissociating iPSCs into single cells and aggregating them into spin embryoid bodies (EBs). We...