A Researcher's guide to rodent models of Down syndrome: Recent insights and translational perspectives [0.03%]
唐氏综合征啮齿动物模型的研究指南：最近的见解和转化视角
Mir A Raza,Andrew Folz,Daniella B Victorino et al.
Mir A Raza et al.
Rodent models of Down syndrome (DS) have been transformative in identifying basic mechanisms underlying the effects of Trisomy 21 at the molecular, cellular, physiological, and neurobehavioral levels. Each model, with its unique genomic arc...
Protocol to induce memory generalization and stress-like behaviors without memory strengthening using contextual fear conditioning in mice [0.03%]
利用情境恐惧条件反射在小鼠中诱导记忆泛化和应激样行为的协议而无需增强记忆
Xin Cheng,Yan Zhao,Yubo Hu et al.
Xin Cheng et al.
Over-generalization of negative memory is common in psychiatric disorders, including depression and anxiety. Here, we present a protocol to dissociate memory generalization from memory strengthening and induce lasting stress-related behavio...
COVID-19 education policy often relied on older research despite available new evidence [0.03%]
新冠肺炎教育政策往往依赖以往的研究成果而忽视新的证据
Basil Mahfouz,Licia Capra,Geoff Mulgan
Basil Mahfouz
Policymakers are increasingly relying on scientific research, but it remains unclear whether policy decisions reflect the latest available evidence. Building on a previously developed semantic matching approach, we use pretrained language-m...
Protocol for detecting causal variants by co-localizing GWAS and QTL studies using colocRedRibbon [0.03%]
一种通过GWAS和QTL研究共定位检测因果变异的colocRedRibbon方案
Theodora Papadopoulou,Aristeidis Sionakidis,Anthony Piron et al.
Theodora Papadopoulou et al.
Here, we present a protocol for using colocRedRibbon to link disease-associated variants to gene expression variation by co-localizing genome-wide association study (GWAS) SNPs with cis-expression quantitative trait loci (eQTLs). We describ...
Protocol for programming DNA crystal morphology using concentration-modulated W/O/W double-emulsion microfluidic droplets [0.03%]
基于浓度调控水/油/水复乳微流体液滴的DNA晶体形态编程协议
Jia Liu,Xugen Chen,Lebing Wang et al.
Jia Liu et al.
Here, we present a protocol for generating concentration-modulated water-in-oil-in-water (W/O/W) double-emulsion droplets (DEDs) to program DNA crystal morphology. We describe assembly of a dual-inner-phase glass capillary microfluidic devi...
A scalable plate reader-based protocol to measure mitochondrial respiration in embryonic hearts [0.03%]
一种基于平板读取器的可扩展协议，用于测量胚胎心脏中的线粒体呼吸作用
Chiemela Ohanele,Jennifer Q Kwong
Chiemela Ohanele
Mitochondrial function is central to embryonic development, yet its assessment in the embryonic heart is hampered by limited tissue availability. Here, we present a protocol to measure mitochondrial oxygen consumption rates directly from em...
Protocol for purifying iPSC-derived retinal pigment epithelial cells by sequential digestion and intrinsic autofluorescence sorting [0.03%]
基于顺序消化和固有自体荧光分选的人诱导多能干细胞来源的视网膜色素上皮细胞纯化方案
Fengming Yang,Rui Li,Xinrun Lv et al.
Fengming Yang et al.
Induced pluripotent stem cells (iPSC)-derived retinal pigment epithelium cells serve as a promising cell source for transplantation therapy in age-related macular degeneration. Here, we present a protocol for purifying the mature retinal pi...
Protocol for ultracentrifugation-based isolation and characterization of extracellular vesicles from FGF2-primed astrocytes [0.03%]
FGF2预处理星形胶质细胞衍生的外泌体超速离心分离及鉴定的标准化方案
Xiaomin Wen,Mingming Wang,Zihao Gao et al.
Xiaomin Wen et al.
Extracellular vesicles (EVs) mediate intercellular communication by transferring bioactive molecular cargo. We present a protocol for isolating EVs from fibroblast growth factor-2 (FGF2)-primed primary mouse astrocytes using differential ul...
Protocol for high-density peptide and protein presentation on biomaterial surfaces using virus-like particles [0.03%]
利用病毒样颗粒在生物材料表面展示高密度多肽和蛋白质的方案
Rayane Hedna,Hasna Maayouf,Thomas Dos Santos et al.
Rayane Hedna et al.
Here, we present a protocol for achieving high-density peptide and protein presentation on biomaterial surfaces using self-assembling virus-like particles. We describe steps for the design, recombinant production, purification, and quality ...
Simultaneous measurement of target and effector cell death, apoptosis, and proliferation in cell therapy development using flow cytometry [0.03%]
采用流式细胞术在细胞治疗开发中同时测量靶细胞和效应细胞的死亡、凋亡及增殖
Kristian Thomson,Kendra Klatt,Niklas Engels et al.
Kristian Thomson et al.
The development of cellular immunotherapies against cancer requires an efficient assessment of malignant target cell killing. However, the fate of effector cells is equally important, particularly in the context of immunomodulatory tumors. ...