Protocol combining RNA interference and regeneration assays in planarian embryos [0.03%]
结合RNA干扰和再生实验的涡虫胚胎协议
Ennis W Deihl,Clare L T Booth,Erin L Davies
Ennis W Deihl
Planarians acquire regenerative abilities during late embryonic and juvenile development. Here, we present a protocol combining gene perturbation by RNA interference (RNAi) and regeneration assays in S. polychroa embryos. We describe steps ...
Protocol for imaging-based quantification of RNAPII clearance during transcription-coupled DNA repair [0.03%]
基于成像的转录偶联DNA修复过程中RNAPII清除量化协议
Bram A F J de Groot,Paula J van der Meer,Diana van den Heuvel et al.
Bram A F J de Groot et al.
Elongating RNA polymerase II (RNAPII) stalls at transcription-blocking lesions in the DNA template strand and is removed by transcription-coupled DNA repair (TCR) factors. Here, we present a protocol to measure RNAPII clearance during TCR a...
Protocol for investigating mitochondrial structure, function, and metabolism in human cervical cancer cells [0.03%]
人宫颈癌细胞线粒体结构、功能和代谢研究方案
Sachin Shetty,Shama Prasada Kabekkodu
Sachin Shetty
Mitochondria regulate a variety of biological activities, including metabolism, oxidative stress, and cell death. Here, we present a protocol for the investigation of mitochondrial structure, function, and metabolism in human cervical cance...
Protocol to clone unknown flanking genomic region using SWPOP-PCR for genome walking [0.03%]
一种利用SWPOP-PCR克隆未知相邻基因组区域的引物构建方法及应用
Qingchun Tang,Kunpeng Chang,Mou Li et al.
Qingchun Tang et al.
Genome walking is a molecular technique for mining unknown flanking DNAs, significantly advancing fields related to biology. We here detail a genome-walking protocol for stepwise partially overlapping primer-based PCR (SWPOP-PCR). We specif...
Protocol for the expression, purification, and biochemical characterization of the innate immune sensor MDA5 [0.03%]
MDA5固有免疫传感器的表达、纯化及生化性质研究方案
Joe D Joiner,Alba Herrero Del Valle,Rahul Singh et al.
Joe D Joiner et al.
MDA5 is one of the primary eukaryotic innate immune sensors of viruses, recognizing long double-stranded RNA (dsRNA). Here, we present procedures for the recombinant expression and purification of murine MDA5 from E. coli. We describe the s...
Protocol to study human Kv1.2 potassium channel pathogenic sequence variants using two-electrode voltage-clamp technique [0.03%]
使用双电极电压钳技术研究人Kv1.2钾通道致病序列变异的协议
Rían W Manville,Geoffrey W Abbott
Rían W Manville
The abietane diterpenoid pisiferic acid (PA) from conifer Chamaecyparis pisifera is a pan-rescuer of human Kv1.2 channel pathogenic loss-of-function (LOF) sequence variants. Here, we present a protocol for the study of Kv1.2 channel sequenc...
Protocol for detection and typing of Serratia marcescens using a qPCR-based technique without cultivation or sequencing [0.03%]
一种无需培养和测序的基于qPCR技术检测并分型沙雷菌的方法
Giorgia Bettoni,Clara Bonaiti,Simona Panelli et al.
Giorgia Bettoni et al.
High-resolution melting (HRM) qPCR is a molecular biology technique for quantifying DNA targets and discriminating them on the basis of their sequences. Hypervariable-locus melting typing (HLMT) exploits HRM for bacterial typing by targetin...
Protocol to measure insulin secretion dynamics from single mouse islets using a microfluidic fluorescence anisotropy immunoassay [0.03%]
基于微流控荧光偏振免疫测定的单个小鼠胰岛分泌动态检测方法
Yufeng Wang,Jonathan V Rocheleau
Yufeng Wang
The dynamics of glucose-stimulated insulin secretion (GSIS) from islets offer valuable insight into β cell metabolism. Here, we present a protocol for using a microfluidic device (i.e., InsC-chip) to measure the temporal dynamics of GSIS f...
Protocol for fabricating a starfish-inspired magnetoelastic generator array [0.03%]
海星磁致伸缩发电机阵列的制备方案
Il Woo Ock,Zhaoqi Duan,Jun Chen
Il Woo Ock
This protocol outlines the fabrication and application of a starfish-inspired magnetoelastic generator (MEG) array for ocean wave energy harvesting and autonomous hydrogen (H2) production. We detail the procedures for device design, frame a...
Protocol for a mouse tubuloid model of myoglobinuric acute kidney injury [0.03%]
肌红蛋白尿急性肾损伤的小鼠管状细胞模型方案
Marc Torres Pereiro,Luping Zhou,Thomas Worzfeld
Marc Torres Pereiro
Myoglobinuric acute kidney injury (AKI) occurs as a result of rhabdomyolysis. Here, we present a protocol based on mouse primary renal tubular epithelial organoids, or "tubuloids", to model myoglobinuric AKI in vitro. We describe how to est...