Protocol for inducing cystitis via direct instillation of acrolein into the bladder of mice [0.03%]
通过直接向小鼠膀胱内灌注丙烯醛诱导膀胱炎的方法学标准流程
Sara A Stuedemann,LaTasha K Crawford
Sara A Stuedemann
Mouse models of bladder inflammation, termed cystitis, can be used to study urinary dysfunction and visceral pain in both sexes. Here, we present a protocol for a mouse model of cystitis that is induced by instilling acrolein directly into ...
A spatially localised cryoinjury protocol to study liver regeneration in zebrafish [0.03%]
一种用于研究斑马鱼肝再生的冷冻损伤局部造模方法
Marcos Sande-Melon,Beth K Logan,Andrew G Cox et al.
Marcos Sande-Melon et al.
Here, we present a protocol for studying liver regeneration in adult zebrafish using a hepatic cryoinjury technique. We detail the surgical procedure to induce localized necrosis using a cryoprobe. We then detail steps for liver harvesting ...
Protocol for identifying surface membrane proteins and their associated proteome from mouse cortical neuron cultures by in situ biotinylation [0.03%]
基于原位生物素标记技术的培养鼠皮层神经元表面膜蛋白及其相关蛋白质组的鉴定方法学研究
Liang Shi,Pritha Bagchi,GiaLinh N Nguyen et al.
Liang Shi et al.
Modifications to the plasma membrane proteome reflect neuronal physiological states. Here, we present a protocol to profile surface proteins from mouse cortical neuron cultures using in situ biotinylation. The workflow includes neuron cultu...
Protocol to uncover the protein interactome of small non-coding vault RNAs through RNA antisense purification coupled to mass spectrometry [0.03%]
通过RNA反义纯化与质谱联用技术揭示小非编码vault RNA的蛋白质相互作用组的协议
Sander B van der Kooij,Jorn E Stok,Annemarthe G van der Veen
Sander B van der Kooij
Vault RNAs (vtRNAs) are a family of four small non-coding RNAs (ncRNAs). Here, we present a protocol to identify protein interactors of vtRNA1-1, vtRNA1-2, and vtRNA1-3 in human lung epithelial cells through RNA antisense purification coupl...
Protocol for iterative indirect immunofluorescence imaging of frozen mouse intestinal tissues [0.03%]
冷冻小肠组织迭代间接免疫荧光成像方案
Shuji Matsuguchi,Aiyan Yau,Ayaka Omori et al.
Shuji Matsuguchi et al.
Here, we present a protocol for iterative indirect immunofluorescence imaging (4i) of frozen tissues (Cryo-4i). We describe the steps for mouse intestinal sample preparation and antibody elution with standard reagents. Avoiding antibody con...
Protocol to reconstitute motor protein clustering on vesicle membranes using a DNA scaffold-based system in vitro [0.03%]
基于DNA支架的体外系统重建囊泡膜上 Motor蛋白聚类的机制研究
Rui Jiang,William O Hancock
Rui Jiang
Cytoskeletal motor proteins drive long-range intracellular transport, but how their organization on vesicle membranes affects motility remains poorly understood. Here, we present a protocol to reconstitute kinesin-1 clustering on synthetic ...
Protocol to generate immunocompetent human heart-macrophage assembloids for the study of immune-cardiac interactions [0.03%]
生成用于研究免疫心脏相互作用的免疫competent人类心脏-巨噬细胞assembloids的协议
Colin O&#x;Hern,Mia Dionise,Sammantha Caywood et al.
Colin O&#x;Hern et al.
Here, we present a protocol to generate fully human pluripotent stem cell (hPSC)-derived human heart-macrophage assembloids. We describe steps for differentiating hPSCs into monocytes, generating cardiac organoids, integrating CD14+ monocyt...
Atopic march protocol involving sequential skin and lung inflammation elicited by epicutaneous Staphylococcus aureus and allergen exposure [0.03%]
金黄色葡萄球菌和过敏原经皮诱导的序贯性皮肤和肺部炎症参与特应性疾病进程
Sabrina N Kline,Laine E Feller,Danielle Wills et al.
Sabrina N Kline et al.
The prevalence of allergic diseases is rising globally, and these diseases often follow a sequential pattern of disease onset in childhood, beginning with atopic dermatitis, followed by food allergy, asthma, and allergic rhinitis. Here, we ...
Protocol for quantifying the oxidative cleavage of amyloid-β using oxygen-18 isotope labeling and LC-MS/MS [0.03%]
使用氧-18同位素标记和LC-MS/MS定量测定淀粉样蛋白-β氧化裂解的协议
Yiying Zhu
Yiying Zhu
Here, we present a protocol for quantifying the oxidative cleavage of amyloid-β using oxygen-18 labeling coupled with high-resolution LC-MS/MS. We describe procedures for amyloid-β peptide preparation, copper(II)/ascorbate-mediated oxidat...
Protocol for the synthesis and characterization of Ru@PCN@PEI nanoparticles with superoxide dismutase catalytic activity [0.03%]
具有超氧化物歧化酶催化活性的Ru@PCN@PEI纳米粒子的合成和表征协议
Meijing Wang,Tao Chen,Xinyi Zeng et al.
Meijing Wang et al.
Bimetallic organic frameworks (BMOFs) possess versatile structural and functional features that enable applications in catalysis and biomedicine. Here, we present a protocol to synthesize Ru@PCN@PEI nanoparticles by loading Ru nanoparticles...