Protocol for haplotype-resolved genome comparison and allele-specific expression analysis across multiple tissues and individuals [0.03%]
基于单倍型的基因组比较和多组织、多人allele-specific表达分析协议
Chenyu Shi,Shikai Liu
Chenyu Shi
Haplotype-resolved genome comparison and allele-specific expression (ASE) analysis are essential for understanding how genomic diversity drives phenotypic variation. However, performing these analyses reproducibly across multiple tissues an...
Protocol for high-resolution mapping of RNA-associated chromatin using chromatin isolation by RNA purification-tag [0.03%]
用于通过RNA纯化富集染色质的染色质免疫沉淀进行高分辨率的RNA相关染色体绘制的协议
Ruishuang Fu,Rongjie Zhao,Huimin Yang et al.
Ruishuang Fu et al.
Mapping the genomic binding sites of long non-coding RNAs (lncRNAs) is essential for understanding their regulatory functions. Here, we present a protocol for integrating RNA hybridization with in situ tagmentation using chromatin isolation...
Qinling Yuan,Alice Amitrano,Debanik Choudhury et al.
Qinling Yuan et al.
Optogenetic approaches enable spatiotemporal control of signaling proteins, yet their integration with microfluidic assays to study confined cell migration remains challenging. Herein, we present a protocol for the optogenetic activation of...
Protocol for induction and quantification of injury-primed axon outgrowth in adult mouse DRG neurons [0.03%]
成人小鼠DRG神经元损伤预处理诱导和定量轴突生长的方案
Eliana Seburn,Brett J Hilton
Eliana Seburn
Adult dorsal root ganglion (DRG) neurons provide a powerful model to study axon growth. Dissociated lumbar L3-L5 DRG neurons extend axons de novo in culture, enabling the measurement of baseline outgrowth. Here, we present a protocol for in...
Protocol for transcriptional profiling of fiber type and regeneration markers in mouse Tibialis Anterior muscle [0.03%]
小鼠胫前肌转录分析的协议：纤维类型和再生标志物
Zuleyha Yildirim,Aurélia Noll,Jean-Christophe Amé et al.
Zuleyha Yildirim et al.
Skeletal muscle integrity and function depend on fiber-type composition and regenerative capacity. Evaluating both components in normal and injured tissues provides valuable insights into subtle declines in muscle function and recovery. We ...
Protocol for selective in vivo labeling of bone marrow plasma cells by intrafemoral antibody injection in mice [0.03%]
小鼠股骨内注射抗体选择性标记骨髓浆细胞的方案
Zhuoyun Tong,Yiming Zhu,Haimei Lv et al.
Zhuoyun Tong et al.
Plasma cells are essential for long-term humoral immunity and are enriched in the bone marrow. Here, we present a protocol for selective labeling of plasma cells within a single mouse femur, using an intrafemoral antibody injection approach...
Protocol for quantifying aberrant features of adult Drosophila midgut stem cells as tumor-prediction biomarkers [0.03%]
量化成人果蝇肠道干细胞异常特征作为预测肿瘤生物标志物的协议
Savvas Teloni,Yiorgos Apidianakis
Savvas Teloni
Here, we present a protocol for quantifying aberrant features of clustered intestinal stem cells (ISCs) in the adult Drosophila midgut, enabling tumor-prediction biomarker assessment. We describe steps for clustered ISC labeling, immunostai...
Protocol for CRISPR genome editing in S. cerevisiae using PCR-based guide insertion [0.03%]
基于PCR的指导插入用于S. cerevisiae CRISPR基因组编辑的协议
Hosein Rostamian,Ethan W Madden,Frank M Kaplan et al.
Hosein Rostamian et al.
Here, we present a CRISPR-Cas9 genome-editing protocol for Saccharomyces cerevisiae that simplifies guide construction and enables rapid guide iteration by replacing restriction/ligation cloning with PCR-based guide installation and seamles...
Protocol to study inflammasome activation in induced pluripotent stem cell-derived macrophages [0.03%]
诱导多能干细胞来源的巨噬细胞中研究炎性小体活化的方案
Chloe M McKee,Melanie Cranston,Emma C McKay et al.
Chloe M McKee et al.
Macrophages are widely used to study inflammasome activity, but current in vitro macrophage models have various limitations. Here, we describe a protocol to differentiate induced pluripotent stem cell (iPSC)-derived macrophages (iMacs) and ...
Protocol to study the antitumor and metabolic effects of intermittent fasting plus cisplatin and metformin in ovarian cancer PDX models [0.03%]
用于研究间歇性禁食联合顺铂和二甲双胍对卵巢癌PDX模型的抗肿瘤及代谢效应的研究方案
Ludovica Perotti,Elena Capellini,Federica Guffanti et al.
Ludovica Perotti et al.
Different dietary regimens are being explored as safe, low-cost approaches to enhance cancer therapy. This protocol describes the evaluation of antitumor and metabolic effects of intermittent fasting (IF) combined with cisplatin and metform...