Protocol for antibody optimization and panel design in high-dimensional multiplexed immunofluorescence imaging [0.03%]
高维多重免疫荧光成像的抗体优化和Panel设计规范
Vrinda Gupta,Madelyn J Abraham,Christophe Goncalves et al.
Vrinda Gupta et al.
The rise of spatial biology technologies is advancing our ability to study cellular interactions within native tissue contexts using antibody-based multiplexed imaging. Here, we present a protocol for designing and optimizing a suitable ant...
Protocol for modeling injury-induced regeneration in retinal organoids and isolating hNRSCs for subretinal transplantation in rd10 mice [0.03%]
视网膜类器官损伤再生建模方案及hNRSCs的分离和下视网膜移植手术方案
Ming Chen,YunXi Zhang,Na Gao et al.
Ming Chen et al.
Human retinal organoids (hROs) provide a platform for modeling injury and assessing regenerative capacity in vitro. Here, we present a protocol for stepwise generation of hROs from human embryonic stem cells (hESCs). We describe steps for m...
Protocol for preparing murine fecal microbiota transplant and fecal microbiota metabolites for in vivo and in vitro use [0.03%]
小鼠粪便微生物移植及粪便代谢物的制备规范（体内外应用）
Catherine M Phelps,Daphne M Rodriguez J,Marlies Meisel
Catherine M Phelps
Accumulating evidence demonstrates that microbiota-derived metabolites can modulate host immune responses and influence complex pathologies. Despite their therapeutic potential, the isolation of fecal microbial metabolites and their scalabl...
Protocol for systematic screening of histone H3/H4 mutants to identify histone residues critical for BCAA-dependent replicative lifespan [0.03%]
系统筛查组蛋白H3/H4突变体以识别支链氨基酸依赖的复制寿命的关键组蛋白残基的方案
Sejung Park,Yan Liu,Seong Hoon Ahn
Sejung Park
Here, we present a protocol for high-throughput replicative lifespan (RLS) screening in Saccharomyces cerevisiae using micromanipulation-based aging assays with growth phenotyping. We describe steps for preparing yeast strains, isolating vi...
Protocol to track single-cell-derived clones using DNA barcoding combined with single-cell RNA sequencing [0.03%]
结合DNA条形码和单细胞RNA测序用于追踪单细胞衍生克隆的协议
Hee Jin Shin,Sahil Sharma,Sarah Kronheim et al.
Hee Jin Shin et al.
Clonal fitness and plasticity drive tumor heterogeneity contributing to disease progression and treatment resistance. Here, we provide a protocol to track the clonal outputs from uniquely marked cells. We describe steps for constructing and...
Protocol for capturing the RNA-binding proteome from plants using orthogonal organic phase separation [0.03%]
用于从植物中捕获RNA结合蛋白组的正交有机相分离协议
Victor A Sánchez-Camargo,Gertjan Kramer,Harrold A van den Burg
Victor A Sánchez-Camargo
RNA-binding proteins (RBPs) regulate many processes related to RNA biogenesis, function, localization, and degradation. Here, we present a protocol for the en masse isolation of RBPs crosslinked to RNA and provide a strategy for subsequent ...
Protocol for quantifying hepatitis B virus transcript abundance and genomic segment distribution from single-cell RNA-seq [0.03%]
单细胞RNA测序量化乙肝病毒转录物丰富度和基因组节段分布的方案
Lina Liu,Liyu Chen,Lingyun Zhou
Lina Liu
Hepatitis B virus (HBV) drives liver disease progression. Here, we present a protocol for sequencing single cells from HBV-infected liver tissue, producing two complementary outputs: quantitative per-cell HBV transcript abundance and a geno...
Protocol to study social transmission of stress in group-housed mice using restraint stress [0.03%]
利用禁锢压力研究群居小鼠中压力的社会传播的协议
Dennis Mink,Dennis Horvath,Michael Basler
Dennis Mink
Here, we present a protocol to study social transmission of stress in group-housed mice using restraint stress. We describe steps for exposing mice to restrained conspecifics separated by an acrylic divider to induce indirect stress respons...
Protocol for recording of neural activity in 3D midbrain organoids using a multiple-electrode array [0.03%]
使用多电极阵列记录三维中脑类器官神经活动的方案
Asiye Malkoç,Diana Riekschnitz,Marcelo Rosato Siri et al.
Asiye Malkoç et al.
Here, we present a protocol for electrophysiological recording from human three-dimensional (3D) organoids using substrates designed for two-dimensional cultures. We describe steps for generating midbrain 3D organoids from human induced plu...
Protocol for transcriptomic and epigenomic analysis of JAK inhibitor sensitivity in IFN-γ-primed human macrophages using ATAC-seq and RNA-seq [0.03%]
使用ATAC-seq和RNA-seq分析IFN-γ预处理的人类巨噬细胞中JAK抑制剂敏感性的转录组和表观基因组分析协议
Geunho Kwon,Junho Noh,Jaehyun Lee et al.
Geunho Kwon et al.
Here, we present a protocol for integrating transcriptomic and epigenomic data to analyze Janus kinase (JAK) inhibitor sensitivity in interferon (IFN)-γ-primed human macrophages using assay for transposase-accessible chromatin using sequen...