Protocol to investigate the role of NETosis in health and disease via a high-throughput neutrophil-like model using quantitative imaging [0.03%]
基于定量成像的高通量中性粒细胞样模型探究健康和疾病状态下NETosis的作用机制
Gavin Li,Sara Versales,Brett Guadagnino et al.
Gavin Li et al.
Neutrophil extracellular traps (NETs) are a crucial host-defense mechanism employed by neutrophils in response to various aggregates and contribute to pathology in diverse diseases, from chronic inflammation to sepsis. Here, we present a pr...
Protocol to determine oligomerization states of proteins in living cells using photon counting histogram analysis [0.03%]
采用光子计数直方图分析法测定活细胞内蛋白质寡聚化状态的协议
Zixiao Li,Tyler Camp,Yushan Li et al.
Zixiao Li et al.
Determining protein oligomerization states in living cells is essential for understanding cell signaling and disease pathology, but it remains technically challenging. Here, we present a protocol for quantifying the oligomeric states of Hal...
Protocol for detecting protein recruitment to clathrin structures in cultured cells [0.03%]
一种在培养细胞中检测蛋白质募集到clathrin结构物中的方法
Laura A Orofiamma,Costin N Antonescu
Laura A Orofiamma
Clathrin-mediated endocytosis internalizes key cell-surface receptors and relies on coordinated recruitment of adaptors and accessory proteins to clathrin structures. Here, we present a protocol for quantifying protein recruitment to diffra...
Protocol for culture of zebrafishdeveloping ovaries as an ex vivo system for investigating early oogenesis and ovarian development [0.03%]
小鼠卵巢发育的体外培养体系构建及应用
Amal Shawahny,Yoel Bogoch,Yaniv M Elkouby
Amal Shawahny
A fundamental understanding of early oogenesis is lacking, and zebrafish serve as an excellent model for investigation, based largely on in vivo approaches. We present a protocol for long-term zebrafish ovary culture that supports physiolog...
Protocol to improve gradient-index lens implantation in the mouse medial prefrontal cortex using NeuralGlider-assisted insertion [0.03%]
一种利用NeuralGlider辅助植入改善小鼠前额叶皮层梯度折射率镜头的方案
Sishir Gautam,Olga M Ocón-Grove,Jenna K Greaser et al.
Sishir Gautam et al.
Gradient-index (GRIN) lens implantation enables deep brain imaging in animal models. Tissue-aspiration based GRIN lens implantation procedures often suffer from low success rates. Here, we present an improved protocol for NeuralGlider-assis...
Siavash Khosravi,Christoph Reuss,Margit Ried et al.
Siavash Khosravi et al.
The culture of porcine retinal explants serves as a valuable cellular model for studying retinal pathologies and their underlying molecular mechanisms. Here, we present a protocol for culturing porcine retinal explants, isolating RNA, and R...
Protocol for adoptive neutrophil transfer to study bone marrow and peripheral blood hematopoietic stem cell kinetics in mice [0.03%]
关于小鼠骨髓和外周血造血干细胞动力学的分叶核中性粒细胞过继输注方案
Carlotta Boscaro,Gian Paolo Fadini,Mattia Albiero
Carlotta Boscaro
The homeostatic clearance of neutrophils regulates the kinetics of hematopoietic stem/progenitor cells (HSPCs) in the bone marrow. We present a protocol for studying the kinetics of HSPCs in mice using adoptive neutrophil transfer. We descr...
Protocol for non-invasive genotyping of axolotls using skin swabbing [0.03%]
用于无创提取水下动物基因型的取皮采样 protocol
Laura Isabella Arbanas,Aida Rodrigo Albors
Laura Isabella Arbanas
Fin clipping is the standard method for sampling axolotl DNA for genotyping, but it requires anesthesia and removing a piece of tissue. Here, we present a non-invasive protocol for axolotl DNA sampling and genotyping using skin swabbing. We...
Protocol for conditional mapping of proximal interactomes of dimeric protein complexes using a BioID-based complementation approach [0.03%]
基于BioID的互补方法用于二聚体蛋白复合物邻近互作组的条件图谱绘制协议
Abishankari Rajkumar,Antoine Gaudreau-Lapierre,Curtis L F Anthony et al.
Abishankari Rajkumar et al.
Here, we provide a protocol for conditional mapping of proximal interactomes of dimeric protein complexes using a BioID-based complementation approach. We describe steps for fusing amino- and carboxyl-terminal fragments of the biotin ligase...
Protocol using AGO-APP for isolation and sequencing of argonaute-bound miRNAs in specific neuronal subtypes of the mouse brain [0.03%]
AGO-APP协议用于小鼠大脑特定神经亚型中与Argonaute结合的miRNA的分离和测序
Surbhi Kapoor,Andrea Erni,Harold Cremer et al.
Surbhi Kapoor et al.
Argonaute protein affinity purification by peptides (Ago-APP) is an immunoprecipitation-based approach that isolates argonaute-bound, putatively active microRNAs (miRNAs) via expression of a T6B peptide. Here, we present a protocol for the ...