Protocol for mapping insertion sites of Tol2 transgenes in zebrafish using TransTag [0.03%]
用于使用TransTag在斑马鱼中绘制Tol2转基因插入位点的方案
Xiaolu Wei,Vijaykumar Naidu,Paige Schneider et al.
Xiaolu Wei et al.
A valuable and widely utilized approach to study gene regulatory networks is through transgenic lines. However, detailed information of transgene insertion sites is often not available, complicating the interpretation of experimental result...
Protocol for the measurement and analysis of human photoencephalographic signals [0.03%]
人类光电反应信号测量与分析协议
Mattia Bonzanni,Nicolas Rouleau,Nirosha J Murugan
Mattia Bonzanni
Photoencephalography, involving the measurement of ultraweak photon emissions (UPEs), is increasingly being recognized for its potential significance in understanding brain activity. We present a protocol for recording, analyzing, and inter...
Protocol for the analysis of proteasome activity, assembly state, and composition in fission yeast extracts using native gels [0.03%]
利用原生凝胶分析裂殖酵母提取物中的蛋白酶体活性、装配状态和组成的方法学协议
Gabriel Ruiz-Romero,Rafael R Daga,Silvia Salas-Pino
Gabriel Ruiz-Romero
The proteasome is a macromolecular complex responsible for degrading short-lived or damaged proteins. Proteasome analysis presents challenges due to its high molecular weight and low stability. Here, we present a protocol to assess proteaso...
Protocol for inferring cell type relationships and linking genomic annotations to cell type hierarchies using CellWalker2 [0.03%]
使用CellWalker2推理细胞类型关系和将基因组注释链接到细胞类型层次结构的协议
Zhirui Hu,Pawel F Przytycki,Katherine S Pollard
Zhirui Hu
Single-cell multiomic data reveal cell type-specific regulatory mechanisms, but comparing hierarchies and regulatory programs across related cell types is challenging. Here, we present a protocol for integrating single-cell data to infer ce...
Protocol for the preparation, execution, and automated analysis via IntelliR of IntelliCage-based mouse experiments [0.03%]
基于Intelli Cage的小鼠实验的准备、执行和通过IntelliR进行自动分析的协议
Martin Hindermann,Vinicius Daguano Gastaldi,Justus Wilke et al.
Martin Hindermann et al.
The rapidly evolving field of rodent behavior research led to the development of the IntelliCage, enabling observer-independent experiments with mice. Here, we present a detailed protocol for IntelliCage-based automated cognitive profiling ...
Protocol for purification, single-particle cryo-EM structure determination, and functional assays of the fungal plasma membrane H+-ATPase [0.03%]
纯化、单粒子冷冻电子显微镜结构测定和真菌质膜H+-ATP酶功能检测的协议
Yue-Ran Ni,Zi-Long You,Lin Bai
Yue-Ran Ni
The fungal plasma membrane H+-ATPase (Pma1) maintains intracellular pH and membrane potential, but its study has been limited by challenges in sample preparation and functional assays. Here, we present a protocol for the endogenous purifica...
Protocol for high-content drug screening using tumor organoids on a 384-pillar plate platform [0.03%]
基于384柱平台的类器官高内涵药物筛选协议
Jung Sook Choi,Min Sang Lee,Myung Jin Song et al.
Jung Sook Choi et al.
Tumor organoids provide physiologically relevant three-dimensional (3D) models that overcome the limitations of two-dimensional cultures in drug screening. Here, we present a protocol for generating compact and reproducible ovarian cancer o...
Protocol for measuring exogenous antigen presentation triggered by membranes of bone marrow-derived dendritic cell purified phagosomes [0.03%]
一种由骨髓来源树突状细胞纯化吞噬体膜触发的外源性抗原呈递测定协议
Facundo Garrido,Franco Nieto,Luis S Mayorga et al.
Facundo Garrido et al.
Dendritic cells are potent antigen-presenting cells that initiate T cell responses by presenting exogenous antigens associated with major histocompatibility complex (MHC) class I and MHC class II molecules. Here, we present a protocol to co...
Protocol to annotate and automate single-cell instance segmentation on stimulated Raman histology using deep learning [0.03%]
基于深度学习的刺激拉曼组织化学单细胞实例分割注释和自动化的协议
Abhishek Bhattacharya,Eric Landgraf,Cheng Jiang et al.
Abhishek Bhattacharya et al.
Stimulated Raman histology (SRH) is a label-free optical imaging technique that can discern molecular components such as lipids and proteins at subcellular spatial resolution without histologic staining. Here, we present a protocol for labe...
Protocol for the generation of low-input Hi-C sequencing libraries of FACS-isolated mitotic cells [0.03%]
荧光激活细胞分选离心体细胞后制备低起始量Hi-C文库的方案
Ashley Nichols,Eralda Salataj,Yujin Choi et al.
Ashley Nichols et al.
High-throughput chromosome conformation capture (Hi-C) is a powerful tool to investigate 3D genome architecture. Here, we present a protocol for preparing low-input Hi-C libraries from mitotic cells isolated by fluorescence-activated cell s...