Protocol for manufacturing photosynthetic cyanobacterial cell-based living building materials [0.03%]
一种用于制造光合蓝藻细胞建筑材料的制备方法协议
Yongjun Son,Jihyeon Min,Jiyoon Park et al.
Yongjun Son et al.
Cement-free and photosynthetic cyanobacterial cell-based living building materials (LBMs) can be manufactured using microbially induced calcium carbonate precipitation (MICP) technology. Here, we present a protocol for cultivating Leptolyng...
Protocol for mapping differential protein-protein interaction networks using affinity purification-mass spectrometry [0.03%]
基于亲和纯化质谱的差异蛋白质相互作用图谱绘制方案
Prashant Kaushal,Manisha R Ummadi,Gwendolyn M Jang et al.
Prashant Kaushal et al.
Proteins congregate into complexes to perform diverse cellular functions. Protein complexes are remodeled by protein-coding mutations or cellular signaling changes, driving phenotypic outcomes in health and disease. We present an affinity p...
Protocol for studying macrophage lipid crosstalk with murine tumor cells [0.03%]
研究巨噬细胞与小鼠肿瘤细胞脂质串扰的方案
Daan J Kloosterman,Martina Farber,Menno Boon et al.
Daan J Kloosterman et al.
Lipid accumulation has recently emerged as a key feature underlying the pro-tumorigenic role of macrophages. Here, we present a workflow to study macrophage lipid crosstalk with tumor cells. We describe steps for the identification, purific...
Protocol for evaluating drug-protein interactions based on fluorescence spectroscopy [0.03%]
基于荧光光谱的药物-蛋白相互作用评价方法学研究
Elaheh Jalali,Javad Sargolzaei
Elaheh Jalali
Investigating the interactions between ligands, such as drugs, and proteins is essential to understand their functions and effects. This fluorescence spectroscopy protocol details how to analyze the effects of drugs on protein structure, in...
Protocol for preparing and using a task to study "delayed safety-seeking behavior" in rats [0.03%]
一种用于研究大鼠“延迟求生行为”的实验任务的建立与应用规范
Junghwa Lee,Sukwoo Choi
Junghwa Lee
The study of defensive behaviors in predicting action outcomes in novel situations without trial and error is limited by the lack of appropriate animal models. Here, we present a protocol for preparing and using a delayed safety-seeking tas...
Protocol to measure human IL-6 secretion from CAR T cell-primed macrophage and monocyte lineage cells in vitro and in vivo using humanized mice [0.03%]
体外和体内测定CAR-T细胞活化巨噬细胞及单核细胞系分泌人IL-6的方案研究
Thao Nguyen,Toshiaki Yoshikawa,Yusuke Ito et al.
Thao Nguyen et al.
Chimeric antigen receptor (CAR) T cell therapy often causes serious toxicities, such as cytokine release syndrome (CRS), mainly due to interleukin-6 (IL-6) secreted by monocyte lineage cells. Here, we describe a protocol to generate anti-CD...
Protocol for muscle fiber type and cross-sectional area analysis in cryosections of whole lower mouse hindlimbs [0.03%]
冷冻横切片下整个小鼠后肢肌肉纤维类型和横截面积分析协议
Edmund Battey,Matthieu Dos Santos,Dipsikha Biswas et al.
Edmund Battey et al.
We outline a protocol to visualize all mouse lower hindlimb skeletal muscles simultaneously. We describe procedures for orientating the whole lower hindlimb in gum tragacanth prior to freezing, simplifying the proceeding experimental steps,...
Protocol to study human gut bacterial communities and rhythmicity ex vivo using a chemostat system [0.03%]
一种使用化学计量系统离体研究人体肠道细菌群落和节律性的方案
Helena Heimes,Deborah Häcker,Hélène Omer et al.
Helena Heimes et al.
Chemostat systems can be used to cultivate complex intestinal microbial communities ex vivo. Here, we present a protocol to transfer bacteria from human fecal material into chemostat systems as well as settings to simulate infant or adult c...
Protocol for single-molecule imaging of transcription and epigenetic factors in human neural stem cell-derived neurons [0.03%]
人神经干细胞来源神经元中转录及表观遗传因子单分子成像技术规范
Yuri Atsumi,Nobuhiko Yamamoto,Noriyuki Sugo
Yuri Atsumi
Single-molecule imaging (SMI) is a powerful approach to quantify the spatiotemporal dynamics of transcription in living cells. Here, we describe a protocol of SMI for transcription and epigenetic factors in human cortical neurons derived fr...
Protocol for the preclinical evaluation of gut barrier function and immune interaction in an HT-29/PBMC co-culture model [0.03%]
HT-29/PBMC共培养模型下肠道屏障功能及免疫互作的药理学评价方法学探索研究protocol
Gwendoline Astre,Laurianne Créchet,Nicolas Pomié et al.
Gwendoline Astre et al.
Here, we present a protocol for the pre-clinical evaluation of gut barrier function and immune interaction in an HT-29/PBMC co-culture model. We describe steps for culturing HT-29 cells and peripheral blood mononuclear cells (PBMCs), assemb...