Protocol for dual metabolomics and proteomics using nanoflow liquid chromatography-tandem mass spectrometry [0.03%]
基于纳流液相色谱-串联质谱的代谢组和蛋白组联合分析方法学
Weiwei Lin,Fatemeh Mousavi,Benjamin C Blum et al.
Weiwei Lin et al.
Nanoflow liquid chromatography-tandem mass spectrometry (nLC-MS) benefits untargeted metabolomics by enhancing sensitivity and integrating proteomics for the same sample. Here, we present a protocol to enable nLC-MS for dual metabolomics an...
Protocol to profile tumor and microenvironment from ovarian cancer patient samples and evaluate cell-based therapy using in vitro killing assays [0.03%]
卵巢癌患者样本的肿瘤和微环境分析方案及体外杀伤试验评估细胞治疗
Yan-Ruide Li,Christopher J Ochoa,Zibai Lyu et al.
Yan-Ruide Li et al.
Ovarian cancer (OC) presents significant challenges due to late diagnosis and high recurrence rates, necessitating a deeper understanding of the molecular and cellular characteristics of OC and the exploration of novel therapeutic approache...
Protocol for high-resolution optical mapping of ex vivo rat heart for electrophysiological studies [0.03%]
用于离体大鼠心脏电生理研究的高分辨率光学制图协议
Yen Ling Sung,Yuan Si Tsai,Chu Ling Chang et al.
Yen Ling Sung et al.
Cardiac optical mapping provides high-resolution spatiotemporal insights into cardiac electrophysiology. Here, we present a protocol for capturing electrical signals in rats using voltage-sensitive dyes and blebbistatin to inhibit muscle co...
Protocol for fabricating optically transparent, pinhole-free NiO thin film for memory devices using the spin-coating technique [0.03%]
一种用于制造记忆设备的具有光学透明性且无针孔NiO薄膜的旋转涂覆工艺协议
Adiba Adiba,Franco Mayanglambam,Tufail Ahmad
Adiba Adiba
Fabricating visibly transparent and uniform metal oxide thin films is a significant challenge. Here, we present a protocol for the fabrication of NiO thin films. We detail the essential steps for substrate preparation. We then describe the ...
Protocol to determine the topology of integral endoplasmic reticulum membrane protein in Saccharomyces cerevisiae [0.03%]
用于确定酵母内质网膜整合蛋白拓扑的协议
Ritika Chaudhary,Vineet Choudhary
Ritika Chaudhary
Here, we present a protocol to determine the topology of Fld1 (few lipid droplets), an integral endoplasmic reticulum (ER) membrane protein in Saccharomyces cerevisiae. We describe steps to generate functional N-terminal GFP and C-terminal ...
Protocol for renal subcapsular islet transplantation in diabetic mice to induce long-term immune tolerance [0.03%]
小鼠肾被膜下移植诱导长期免疫耐受的异体胰岛移植术式
Yinglin Yuan,Qibin Wu,Guoli Huai et al.
Yinglin Yuan et al.
Murine renal subcapsular islet transplantation presents a promising technique for diabetes treatment by addressing challenges such as immune rejection and reliance on immunosuppressive drugs. Here, we present a protocol for the isolation, p...
Protocol for the isolation of human neutrophil-derived extracellular vesicles for characterization and functional experimentation [0.03%]
人源中性粒细胞来源胞外囊泡的分离、鉴定及功能实验操作规范
Robert Richter,Ezgi Sari,Delores Stacks et al.
Robert Richter et al.
Small extracellular vesicles (EVs) derived from neutrophils play an important role in the remodeling of extracellular matrices in human health and disease. Here, we present a protocol for isolating EVs secreted from human neutrophils either...
Kathiresan Purushothaman,Saraphina Dianne Tneo Rwei Qing,Yong Xin Ee et al.
Kathiresan Purushothaman et al.
Zebrafish is a key model for studying vertebrate development and human diseases, but proteomic data during embryogenesis are limited due to interference from egg yolk proteins. Here, we present a protocol for the isolation, identification, ...
Protocol for the establishment of a mouse myocardial infarction and ischemia-reperfusion model via heart compression [0.03%]
小鼠心脏压迫诱导心肌梗死及缺血再灌注模型制备方案
Chongning Zhong,Hui Dong,Xianwu Cheng et al.
Chongning Zhong et al.
Myocardial infarction (MI) and myocardial ischemia-reperfusion injury (MIRI) are major pathological conditions in cardiovascular disease, requiring in-depth study for effective therapy development. Here, we present a detailed protocol for e...
Protocol for observing tunneling nanotube formation and function in both fixed and live primary mouse neurons and microglia coculture system [0.03%]
用于观察小胶质细胞与原代鼠神经元共培养系统中隧道纳米管形成和功能的协议
Vivian Baker,Dimitri Budinger,Sean-Patrick Riechers et al.
Vivian Baker et al.
Microglia and neurons can connect via tunneling nanotubes (TNTs), facilitating the transfer of organelles, vesicles, and proteins. Here, we present a protocol for visualizing murine TNT formation and material transfer between neurons and mi...