Protocol for assaying irreversible inhibitors of thioredoxin reductase 1 [0.03%]
测定不可逆抑制剂抑制硫氧还蛋白还原酶1活性的方案
Shibo Sun,Haowen Liu,Wuyang Shi et al.
Shibo Sun et al.
Selenoprotein thioredoxin reductase 1 (TXNRD1) is a promising therapeutic target, with several inhibitors reported to inhibit TXNRD1 activity. These inhibitors have the potential for applications such as anti-tumor medications. Here, we pre...
Protocol for enriching, isolating, and testing drug susceptibility of facultative anaerobic fungi [0.03%]
兼性厌氧真菌富集、分离及药物敏感性检测方案
Krishna K Yadav,Om Prakash
Krishna K Yadav
Research on fungi under anaerobic conditions is limited but crucial for understanding their ecological and pathological impacts. Here, we present a protocol for enriching, isolating, and characterizing anaerobic fungi from environmental and...
Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation [0.03%]
利用超速离心从白血病的临床前模型脾脏中分离白血病来源的外泌体的流程
Ernesto Gargiulo,Pablo Elias Morande,Maxmilan Jeyakumar et al.
Ernesto Gargiulo et al.
Here, we present a protocol for the direct isolation of small extracellular vesicles (sEVs) from the spleen of preclinical murine models of leukemia using ultracentrifugation. We describe steps for tissue collection, sample preparation, ult...
Protocol to evaluate translation inhibition in Candida species using fluorescence microscopy and flow cytometry [0.03%]
采用荧光显微镜和流式细胞术评估白色念珠菌等物种翻译抑制的协议
Emily Puumala,Nicole Robbins,Leah E Cowen
Emily Puumala
Translation inhibitors have therapeutic potential against Candida species. Here, we present a protocol to measure translation inhibition in Candida spp. We describe steps for employing an alkynylated methionine analog, L-homopropargylglycin...
Protocol to induce neurodegeneration in a local area of the mouse brain by stereotaxic injection [0.03%]
立体定位注射诱导小鼠脑局部神经退行性病变的方法
Xuebing Zhang,Qingqing Lu,Xingqi Meng et al.
Xuebing Zhang et al.
In vivo models of brain pathology are crucial for studying neurological diseases. Here, we present a protocol to induce a pathological condition in a mouse brain area by local injection of neurotoxic stimulus. We describe steps for preparin...
Protocol for establishing spontaneous metastasis in mice using a subcutaneous tumor model [0.03%]
使用皮下肿瘤模型在小鼠中建立自发性转移的方案
Shiqin Liu,Michelle Shen,Kewei Le et al.
Shiqin Liu et al.
Recapitulating spontaneous metastasis in preclinical models is crucial for understanding mechanisms underlying cancer progression and testing effective therapeutic interventions. We present a protocol for establishing and characterizing the...
Protocol to identify and isolate rare murine tumor-resident dendritic cell populations for low-input transcriptomic profiling [0.03%]
识别、分离小鼠肿瘤驻留树突状细胞群体并进行低输入转录组分析的协议
Athanasios Papadas,Daniel J Lagal,Yaling Dou et al.
Athanasios Papadas et al.
Conventional type 1 dendritic cells (cDC1s) are critical for innate sensing of cancer, yet they are scarce in the tumor microenvironment (TME). Here, we present a protocol to identify and isolate cDC1 subsets from murine implantable tumors ...
Protocol to achieve high-resolution single-cell transcriptomics of cardiomyocytes in multiple species [0.03%]
多物种心肌细胞高分辨率单细胞转录组的实验实现协议
Ditte Gry Ellman,Frederik Adam Bjerre,Sara Thornby Bak et al.
Ditte Gry Ellman et al.
Single-cell RNA sequencing (scRNA-seq) remains state-of-the-art for transcriptomic cell-mapping. Here, we provide a protocol to generate high-resolution scRNA-seq of rare cardiomyocyte populations (e.g., regenerating/dividing, etc.) from mo...
Robert Kousnetsov,Daniel Hawiger
Robert Kousnetsov
Seqtometry (sequencing-to-measurement) is an analytical platform for single-cell analysis based on direct profiling of gene expression and accessibility achieved by advanced scoring with gene signatures. Here, we present a protocol for sing...
Protocol for siRNA-mediated U1 snRNA knockdown using fluorinated α-helical polypeptide in vitro and in vivo [0.03%]
用于体内外siRNA介导的U1 snRNA敲低的氟化α-螺旋多肽的方案
Fan Zhang,Chenglong Ge,Zigang Qiao et al.
Fan Zhang et al.
Here, we present a protocol for small interfering RNA (siRNA)-mediated U1 small nuclear RNA (snRNA) knockdown using fluorinated α-helical polypeptide in macrophages and mouse lungs, providing a dependable approach to silence U1 snRNA in vi...