Protocol for ultrasound-guided injection into the murine portal vein to initiate liver metastasis [0.03%]
小鼠超声引导下门静脉注射造模指南
Nicole Henning,Fred Roberts,Virginia Spanoudaki
Nicole Henning
Here, we present a non-surgical protocol for modeling murine liver metastasis by disseminating injected cells or compounds into the murine portal vein. We describe steps for identifying the portal vein and other vital structures adjacent to...
Protocol for semi-automatic quantitative bioimaging analysis of synapse loss [0.03%]
半自动定量生物影像分析突触丢失的协议
Mariana A Barata,Cláudia Guimas Almeida
Mariana A Barata
Synapse loss correlates with cognitive decline in neurodegenerative diseases like late-onset Alzheimer's disease. We developed a semi-automated workflow to quantify synapse loss in primary mouse neurons. The protocol includes culturing neur...
Protocol for integrated ubiquitination analysis of in vitro E3 ligase-DUB regulation and in vivo ubiquitin chain linkage characterization [0.03%]
体外E3泛素连接酶-去泛素化酶调节及体内泛素链链接表征的整合泛素化分析协议
Daniela Mennerich,Carlos Vela-Rodriguez,Lari Lehtiö et al.
Daniela Mennerich et al.
Here, we present a protocol for the recombinant production of DTX3L and USP28, along with in vitro and in vivo procedures for examining their regulatory functions. We describe steps for reconstituting DTX3L-dependent ubiquitination in vitro...
Protocol for constructing multi-ancestry polygenic models using S4-Multi [0.03%]
基于S4-Multi的多祖先群体基因组多基因评分模型构建规范
Ping-Hung Lai,Jonathan P Tyrer,John Baierl et al.
Ping-Hung Lai et al.
Here, we present a protocol to construct multi-ancestry polygenic models using S4-Multi and generate polygenic risk scores. We describe steps for preparing reference genotype files, formatting genome-wide association stud (GWAS) summary sta...
Protocol for inducing deep vein thrombosis in C57BL/6J mice using the inferior vena cava stenosis model [0.03%]
C57BL/6J小鼠下腔静脉狭窄模型诱导深静脉血栓形成的方法学探索
Aman Damara,Santiago Canon,Magdalena L Bochenek et al.
Aman Damara et al.
Here, we present a protocol for inducing deep vein thrombosis (DVT) in C57BL/6J mice using the inferior vena cava (IVC) stenosis model. We describe steps for surgical preparation, IVC ligation, and non-invasive detection and confirmation of...
Lydia Teboul,Benjamin Davies
Lydia Teboul
This primer manuscript summarizes gene-editing strategies for limiting gene-editing activity with the aim of avoiding biallelic disruption. Mono-allelic editing of cells and embryos is advantageous when modeling dominant genetic disorders o...
Protocol for longitudinal two-photon calcium imaging and holographic optogenetic manipulation to investigate memory in mice [0.03%]
小鼠记忆研究的双光子钙成像和全息光遗传学操作纵向研究方案
Huaiqin Guo,He Yang,Feng Su et al.
Huaiqin Guo et al.
Here, we present a protocol for longitudinal two-photon calcium imaging and holographic optogenetic manipulation to investigate memory in mice. We describe chronic cranial window implantation, stereotaxic viral delivery, and head-fixed cont...
Protocol for identifying functional regulatory mutation blocks by integrating genome sequencing and transcriptome data [0.03%]
通过整合基因组测序和转录组数据识别功能调控变异区块的方案
Mingyi Yang,Gege Liu,Magnar Bjørås et al.
Mingyi Yang et al.
Identifying functional mutation blocks (FMBs) that contribute to genome-wide transcriptional regulation remains a challenge. BayesPI-BAR version 3 (bpb3) is a Python-based tool for predicting FMBs by integrating DNA sequence data with gene ...
Wenyu Han,Xiaofeng Li,Junyan Lin et al.
Wenyu Han et al.
Liquid-liquid phase separation (LLPS) of ARID3B is critical in nonsyndromic cleft lip/palate pathogenesis. Here, we present a protocol to visualize ARID3B LLPS. We describe steps for regenerating LLPS with purified protein in vitro and dete...
Protocol for isolating human extracellular vesicles from nephrotic urine and enriching autoimmunoglobulin-triggered extracellular vesicles [0.03%]
从大量蛋白尿中分离人外泌体并富集自身免疫球蛋白触发的外泌体的实验方法
Karen Lahme,Catherine Meyer-Schwesinger
Karen Lahme
This protocol describes the isolation of total urinary extracellular vesicles (EVs) from nephrotic patient urines and enrichment of AutoImmunoglobulin-Triggered Extracellular Vesicles (AIT-EVs). Procedures for urine collection, sample proce...