Protocol to study the contribution of biomechanics in osteoarthritis development using a cartilage-on-chip model [0.03%]
基于软骨芯片模型研究生物力学在骨关节炎发生发展中作用的实验方案
Elodie Faure,Veronique Chobaz,Diego de Haro et al.
Elodie Faure et al.
Here, we present a protocol to study biomechanics and calcification in osteoarthritis using a cartilage-on-chip model. We describe steps for culturing human chondrocytes in 3D hydrogels and subjecting them to physiological loading. We then ...
Protocol for single-use Ambu aScope-based minimally invasive bronchoalveolar lavage collection in pigs [0.03%]
基于Ambu aScope的一次性经支气管镜肺泡灌洗小猪模型的操作规范
Piyush Baindara,Dinata Roy,Addison K Byrne et al.
Piyush Baindara et al.
Here, we present a protocol for collecting bronchoalveolar lavage (BAL) samples from live pigs using a single-use Ambu aScope. We describe the steps for animal preparation, BAL collection, and sample handling, including downstream analysis,...
Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei [0.03%]
用于识别志贺菌核苷酸第二信使信号网络的方案
Binbin Cui,Xiayu Chen,Mingfang Wang et al.
Binbin Cui et al.
Here, we present a protocol to detect the signaling network composed of cyclic AMP (cAMP) and cyclic di-GMP (c-di-GMP) signaling systems in Shigella sonnei. Technical assays include microscale thermophoresis (MST), quantitative reverse-tran...
Protocol for rapid tRNA enrichment and chemiluminescent northern blot detection of tRNA and tRNA-derived fragments [0.03%]
tRNA富集和tRNA及其衍生片段的化学发光Northern印迹检测 protocol
Pavlina Gregorova,Minna-Maria K Heinonen,Milla M Laarne et al.
Pavlina Gregorova et al.
Transfer RNA (tRNA), its post-transcriptional modifications, and tRNA-derived fragments (tRFs) play essential roles in cellular processes and gene regulation. Here, we present a fast and efficient tRNA enrichment protocol using silica spin ...
Protocol for non-invasive tumor monitoring and diagnosis based on interpretable deep learning [0.03%]
基于可解释的深度学习的非侵入性肿瘤监测和诊断协议
Zhenbo Yuan,Yuli Yan,Youpeng Yang et al.
Zhenbo Yuan et al.
Tumor-specific DNA methylation profiling in plasma cell-free DNA (cfDNA) offers a promising approach for non-invasive tumor detection. Here, we present a protocol that uses Oncoder, an interpretable deep-learning-based framework, to monitor...
Protocol for simultaneous profiling of N- and O-glycans on glycoproteins using a one-pot format [0.03%]
基于单管反应体系的同时分析糖蛋白N-和O-连接型聚糖的方法学协议
Kevan T Knizner,Darren T Gass,Yasuyuki Matsumoto et al.
Kevan T Knizner et al.
Here, we present a protocol to simultaneously release and profile N- and O-glycans from glycoproteins in a one-pot format. We describe steps for protein reduction, alkylation, N-glycan release, Proteinase K digestion, HILIC enrichment, N-gl...
Protocol for non-invasive delivery of CRISPR RNPs via virus-like particles for mouse model generation [0.03%]
用于小鼠模型构建的通过病毒样颗粒非侵入性递送CRISPR RNPs的方案
Da Eun Yoon,Jiyun Yang,Tae Yeong Jeong et al.
Da Eun Yoon et al.
CRISPR-virus-like particle (VLP)-induced targeted mutagenesis (CRISPR-VIM) enables genome editing in mouse embryos through non-invasive delivery of CRISPR ribonucleoproteins (RNPs) via VLPs, eliminating the need for physical manipulation an...
Protocol for the quantitative imaging and segmentation of spatial patterns in Hibiscus trionum petals [0.03%]
Hibiscus trionum花瓣空间图案定量成像和分割的协议
Lucie Riglet,Edwige Moyroud
Lucie Riglet
Most developmental processes underpinning the diversity of floral forms remain unexplored due to limitations in current imaging approaches. Here, we present a protocol to spatially analyze tissue-wide cell growth dynamics through confocal i...
Protocol for identifying properties of ERBB receptor antagonists using the barcoded ERBBprofiler assay [0.03%]
用于识别ERBB受体拮抗剂特性的条形码ERBB检测方法协议
Lukša Popović,Moritz J Rossner,Michael C Wehr
Lukša Popović
Published Erratum
STAR protocols. 2026 Feb 3;7(1):104372. DOI:10.1016/j.xpro.2026.104372 2026
A high-parameter spectral cytometry protocol for immune subset and functional characterization of cryopreserved human blood with 49 biomarkers [0.03%]
一种高参数谱域细胞计数术：使用49种生物标志物对冷冻的人类血液的免疫亚型和功能进行表征的方法
Austin P Wright,Marissa Lindaman,Elaine Huang et al.
Austin P Wright et al.
Comprehensive immune profiling of human blood is a critical approach for evaluating immune responses following therapeutic intervention in clinical trials. Here, we present a protocol to assess leukocyte lineages, activation status, and sel...