Development of reusable screen-printed ion-selective electrodes with calibration-free operation [0.03%]
一种可重复使用的丝网印刷离子选择性电极的开发及其无需标定的操作特性
Pavel Sengupta,Chloe Ramsperger,Dipankar Koley
Pavel Sengupta
The reusable solid-state calibration-free screen-printed ion-selective electrodes (SP-ISEs) have been developed with a carbon paste and PEDOT: PEDOT-S (S=sulfonated) back contact. The calibration-free Na+ and Ca2+ SP-ISEs showed a near Nern...
Harsha Amarasekara,Riley Lehman,Paniz Rezvan Sangsari et al.
Harsha Amarasekara et al.
Nucleic acid detection is commonly used to diagnose pathogenic and genetic diseases. Diagnostics based on polymerase chain reaction (PCR) are routinely used to amplify and signal the presence of a target nucleic acid, but PCR-based methods ...
FRET-based reporter assesses lysosomal DNA-degradation ability in live cells [0.03%]
基于FRET的报告基因检测活细胞中溶酶体DNA降解能力
Jared Morse,Ka Ho Leung
Jared Morse
Lysosomes are multifunctional organelles that serve as the cell's central hub for metabolic signaling. Lysosomal malfunction disrupts intracellular homeostasis, leading to adverse health effects. Therefore, assessing lysosomal function is v...
Yu Chang,Can Wang,Ke Du
Yu Chang
High resolution 3D printing emerges as an alternative to microfabrication due to its fine resolution along with one-step manufacturing. Thus, it is broadly used in many fields, such as biological and chemical applications. We introduce such...
Non-enzymatic glucose detection via ordered 2D arrays of nickel and nickel chitosan nanowires [0.03%]
有序镍和镍壳聚糖纳米线二维阵列用于非酶葡萄糖检测
Vicente Gonzales,Chris Rightsell,Alejandro Morales Betancourt et al.
Vicente Gonzales et al.
Glucose sensors play a vital role in the everyday healthcare needs of diabetic patients. However, glucose sensor cost and reliability, particularly regarding their standard functionalization with expensive and environmentally sensitive enzy...
Resistive Pulse Sensing of Pre-Nucleation Activities during Single-Entity Lysozyme Crystallization on Single Nanopipettes [0.03%]
单纳米pipette上单实体溶菌酶结晶过程中预成核活性的阻抗脉冲检测法研究
Yusuff Balogun,Ruoyu Yang,Gangli Wang
Yusuff Balogun
The formation of cluster aggregates in a (super)saturated solution prior to protein nucleation is crucial to overcoming the thermodynamic energy barrier which enables further growth of single crystals. This process is important for single c...
Prussian blue nanofilm-sensitized plasmonic electrochemical microscopy for spatially resolved detection of the localized delivery of hydrogen peroxide [0.03%]
用于检测局部递送氢过氧化物的空间分辨的普鲁士蓝纳米膜感光等离子体电化学显微镜
Adaly Garcia,Christina Dhoj,Samuel Groysman et al.
Adaly Garcia et al.
Hydrogen peroxide (H2O2) sensing has been widely investigated using various electrochemical methods, yet the challenge of finding an imaging technique capable of real-time, spatially resolved detection remains. Addressing this, we introduce...
Rapid and portable quantification of HIV RNA via a smartphone-enabled digital CRISPR device and deep learning [0.03%]
基于智能手机的CRISPR设备和深度学习实现HIV RNA的快速定量检测
Hoan T Ngo,Patarajarin Akarapipad,Pei-Wei Lee et al.
Hoan T Ngo et al.
For the 29.8 million people in the world living with HIV/AIDS and receiving antiretroviral therapy, it is crucial to monitor their HIV viral loads. To this end, rapid and portable diagnostic tools that can quantify HIV RNA are critically ne...
Patterning amyloid-β aggregation under the effect of acetylcholinesterase using a biological nanopore - an in vitro study [0.03%]
乙酰胆碱酯酶影响下用生物纳米孔调控淀粉样蛋白-β聚集的体外研究
Nandhini Subramanian,Brittany Watson,Chen-Zhong Li et al.
Nandhini Subramanian et al.
Aggregation of amyloid-β peptide (Aβ) is hypothesized to be the primary cause of Alzheimer's disease (AD) progression. Aβ aggregation has been widely studied using conventional sensing tools like emission fluorescence, electron microscop...
A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer [0.03%]
用于SARS-COVID19的自我组装裂分适体多重检测及基于DNA的孔雀绿适体的小型化
Martin R O&#x;Steen,Dmitry M Kolpashchikov
Martin R O&#x;Steen
Multiplex assays often rely on expensive sensors incorporating covalently linked fluorescent dyes. Herein, we developed a self-assembling aptamer-based multiplex assay. This multiplex approach utilizes a previously established split aptamer...