Putting on molecular weight: Enabling cryo-EM structure determination of sub-100-kDa proteins [0.03%]
提高分子量：使小于100kDa蛋白质的冷冻电镜结构测定成为可能
Koen Wentinck,Christos Gogou,Dimphna H Meijer
Koen Wentinck
Significant advances in the past decade have enabled high-resolution structure determination of a vast variety of proteins by cryogenic electron microscopy single particle analysis. Despite improved sample preparation, next-generation imagi...
Binding properties of the anti-TB drugs bedaquiline and TBAJ-876 to a mycobacterial F-ATP synthase [0.03%]
贝达喹啉和TBAJ-876与结核分枝杆菌F-ATP合酶的结合性质研究
Alexander Krah,Gerhard Grüber,Peter J Bond
Alexander Krah
Tuberculosis (TB), the deadly disease caused by Mycobacterium tuberculosis (Mtb), kills more people worldwide than any other bacterial infectious disease. There has been a recent resurgence of TB drug discovery activities, resulting in the ...
Regulation of the interactions between human eIF5 and eIF1A by the CK2 kinase [0.03%]
CK2激酶对人类eIF5和eIF1A相互作用的调节作用
Nathan Gamble,Eleanor Elise Paul,Bibin Anand et al.
Nathan Gamble et al.
Translation initiation in eukaryotes relies on a complex network of interactions that are continuously reorganized throughout the process. As more information becomes available about the structure of the ribosomal preinitiation complex (PIC...
Comparative computational and experimental analyses of some natural small molecules to restore transcriptional activation function of p53 in cancer cells harbouring wild type and p53Ser46 mutant [0.03%]
计算和实验分析一些天然小分子在携带野生型和P53Ser46突变的癌细胞中恢复p53转录激活功能的比较研究
Seyad Shefrin,Anissa Nofita Sari,Vipul Kumar et al.
Seyad Shefrin et al.
Genetic mutations in p53 are frequently associated with many types of cancers that affect its stability and activity through multiple ways. The Ser46 residue present in the transactivation domain2 (TAD2) domain of p53 undergoes phosphorylat...
Diego S Vazquez,Pamela L Toledo,Alejo R Gianotti et al.
Diego S Vazquez et al.
Protein conformation and cell compartmentalization are fundamental concepts and subjects of vast scientific endeavors. In the last two decades, we have witnessed exciting advances that unveiled the conjunction of these concepts. An avalanch...
HGDiscovery: An online tool providing functional and phenotypic information on novel variants of homogentisate 1,2- dioxigenase [0.03%]
HGDiscovery：一个针对同雄激素1,2-双氧酶新变异体功能和表型信息的在线工具
Malancha Karmakar,Vittoria Cicaloni,Carlos H M Rodrigues et al.
Malancha Karmakar et al.
Alkaptonuria (AKU), a rare genetic disorder, is characterized by the accumulation of homogentisic acid (HGA) in the body. Affected individuals lack functional levels of an enzyme required to breakdown HGA. Mutations in the homogentisate 1,2...
Engineering enhanced thermostability into the Geobacillus pallidus nitrile hydratase [0.03%]
工程化增强热稳定性到地质杆菌苍白亚种腈水合酶中
Jennifer C Van Wyk,B Trevor Sewell,Michael J Danson et al.
Jennifer C Van Wyk et al.
Nitrile hydratases (NHases) are important biocatalysts for the enzymatic conversion of nitriles to industrially-important amides such as acrylamide and nicotinamide. Although thermostability in this enzyme class is generally low, there is n...
Biochemical and structural analysis of a cytosolic sulfotransferase of the malaria vector Anopheles gambiae overexpressed in the reproductive tissues [0.03%]
生化和结构分析疟疾媒介按蚊细胞质硫酸转移酶及其在生殖组织中的过表达
Arianna Esposito Verza,Riccardo Miggiano,Fabrizio Lombardo et al.
Arianna Esposito Verza et al.
The temporary or permanent chemical modification of biomolecules is a crucial aspect in the physiology of all living species. However, while some modules are well characterised also in insects, others did not receive the same attention. Thi...
Metabolically-incorporated deuterium in myelin localized by neutron diffraction and identified by mass spectrometry [0.03%]
利用中子衍射定位并用质谱仪鉴定的髓鞘中的代谢掺入氘原子
Anne Baumann,Andrew R Denninger,Marek Domin et al.
Anne Baumann et al.
Myelin is a natural and dynamic multilamellar membrane structure that continues to be of significant biological and neurological interest, especially with respect to its biosynthesis and assembly during its normal formation, maintenance, an...
Izaak N Beck,David M Picton,Tim R Blower
Izaak N Beck
Bacteria are constantly challenged by bacteriophage (phage) infection and have developed multitudinous and varied resistance mechanisms. Bacteriophage Exclusion (BREX) systems protect from phage infection by generating methylation patterns ...