Cryo-EM structure of substrate-free E. coli Lon protease provides insights into the dynamics of Lon machinery [0.03%]
无底物时的Lon蛋白酶的低温EM结构为研究Lon蛋白酶的动力学提供了线索
Istvan Botos,George T Lountos,Weimin Wu et al.
Istvan Botos et al.
Energy-dependent Lon proteases play a key role in cellular regulation by degrading short-lived regulatory proteins and misfolded proteins in the cell. The structure of the catalytically inactive S679A mutant of Escherichia coli LonA proteas...
Thermodynamics and folding landscapes of large proteins from a statistical mechanical model [0.03%]
大型蛋白质的统计力学模型热力学和折叠势能景观
Soundhararajan Gopi,Akashnathan Aranganathan,Athi N Naganathan
Soundhararajan Gopi
Statistical mechanical models that afford an intermediate resolution between macroscopic chemical models and all-atom simulations have been successful in capturing folding behaviors of many small single-domain proteins. However, the applica...
The crystal structure of the β subunit of luteinizing hormone and a model for the intact hormone [0.03%]
卵泡刺激素β亚基的晶体结构及完整激素模型
Steven B Larson,Alexander McPherson
Steven B Larson
The β subunit of bovine luteinizing hormone (LH) was crystallized and its structure solved to 3.15 Å resolution by molecular replacement using human chorionic gonadotropin (hCG) β subunit as search model. The asymmetric unit contains t...
Joshua Temple,Therese N Tripler,Qi Shen et al.
Joshua Temple et al.
From cellular deposition of the HIV-1 capsid to integration of the viral genome, the capsid constitutes a primary target of a variety of host proteins that work to either promote or inhibit HIV-1 infection. Successful progression of HIV-1 i...
Integrative structural biology studies of HIV-1 reverse transcriptase binding to a high-affinity DNA aptamer [0.03%]
HIV-1反转录酶与高亲和力DNA适配子结合的集成结构生物学研究
Steve Tuske,Jie Zheng,Erik D Olson et al.
Steve Tuske et al.
The high-resolution crystal structure of HIV-1 reverse transcriptase (RT) bound to a 38-mer DNA hairpin aptamer with low pM affinity was previously described. The high-affinity binding aptamer contained 2'-O-methyl modifications and a seven...
One-step sequence and structure-guided optimization of HIV-1 envelope gp140 [0.03%]
一步法HIV-1包膜gp140序列和结构导向优化
Sameer Kumar Malladi,David Schreiber,Ishika Pramanick et al.
Sameer Kumar Malladi et al.
Stabilization of the metastable envelope glycoprotein (Env) of HIV-1 is hypothesized to improve induction of broadly neutralizing antibodies. We improved the expression yield and stability of the HIV-1 envelope glycoprotein BG505SOSIP.664 g...
Juliet O Obi,Mulu Y Lubula,Gabriel Cornilescu et al.
Juliet O Obi et al.
Bromodomain-containing proteins are often part of chromatin-modifying complexes, and their activity can lead to altered expression of genes that drive cancer, inflammation and neurological disorders in humans. Bromodomain-PHD finger protein...
Dual targeting of 3CLpro and PLpro of SARS-CoV-2: A novel structure-based design approach to treat COVID-19 [0.03%]
针对SARS-CoV-2的3CLpro和PLpro的双重靶向：一种新颖的基于结构的设计方法以治疗COVID-19
Sajjan Rajpoot,Manikandan Alagumuthu,Mirza S Baig
Sajjan Rajpoot
With the rapid growth of the COVID-19 (coronavirus disease 2019) pandemic across the globe, therapeutic attention must be directed to fight the novel severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). However, developing new anti...
Zhou Yin,Harikanth Venkannagari,Haley Lynch et al.
Zhou Yin et al.
ΔFosB is a highly stable transcription factor that accumulates in specific brain regions upon chronic exposure to drugs of abuse, stress, or seizures, and mediates lasting behavioral responses. ΔFosB reportedly heterodimerizes with JunD f...