Juliane F Ripka,Albert Perez-Riba,Piyush K Chaturbedy et al.
Juliane F Ripka et al.
Alpha-helical repeat proteins such as consensus-designed tetratricopeptide repeats (CTPRs) are exceptionally stable molecules that are able to tolerate destabilizing sequence alterations and are therefore becoming increasingly valued as a m...
Structural and binding characterization of the LacdiNAc-specific adhesin (LabA; HopD) exodomain from Helicobacter pylori [0.03%]
幽门螺杆菌半乳糖二酰胺-N-乙酰氨基葡萄糖神经氨酸配基（LabA；HopD）外结构域的结构和结合特性分析
Vasiliki Paraskevopoulou,Marianne Schimpl,Ross C Overman et al.
Vasiliki Paraskevopoulou et al.
Helicobacter pylori (H. pylori) uses several outer membrane proteins for adhering to its host's gastric mucosa, an important step in establishing and preserving colonization. Several adhesins (SabA, BabA, HopQ) have been characterized in te...
Crystal structure of the atypically adhesive SpaB basal pilus subunit: Mechanistic insights about its incorporation in lactobacillar SpaCBA pili [0.03%]
非典型粘附蛋白SpaB芽孢外膜素的晶体结构：其在乳杆菌系SpaCBA芽孢外膜素中的整合机制的见解
Abhin Kumar Megta,Shivendra Pratap,Abhiruchi Kant et al.
Abhin Kumar Megta et al.
To successfully colonize a host or environment, certain genera and species of Gram-positive bacteria have evolved to utilize the so-called sortase-dependent pilus, a long multi-subunit and non-flagellar surface adhesin. One example of this ...
A two-stage computational approach to predict novel ligands for a chemosensory receptor [0.03%]
预测化学感觉受体的新配体的两阶段计算方法
Amara Jabeen,Ramya Vijayram,Shoba Ranganathan
Amara Jabeen
Olfactory receptor (OR) 1A2 is the member of largest superfamily of G protein-coupled receptors (GPCRs). OR1A2 is an ectopically expressed receptor with only 13 known ligands, implicated in reducing hepatocellular carcinoma progression, wit...
Shotgun EM of mycobacterial protein complexes during stationary phase stress [0.03%]
静息状态下分枝杆菌蛋白复合物的EM质谱分析
Angela M Kirykowicz,Jeremy D Woodward
Angela M Kirykowicz
There is little structural information about the protein complexes conferring resistance in Mycobacterium tuberculosis (Mtb) to anti-microbial oxygen and nitrogen radicals in the phagolysosome. Here, we expose the model Mycobacterium, Mycob...
Wei-Ven Tee,Enrico Guarnera,Igor N Berezovsky
Wei-Ven Tee
Studies of protein allostery increasingly reveal an involvement of the back and forth order-disorder transitions in this mechanism of protein activity regulation. Here, we investigate the allosteric mechanisms mediated by structural disorde...
Structure dictates the mechanism of ligand recognition in the histidine and maltose binding proteins [0.03%]
结构决定组氨酸和麦芽糖结合蛋白配体识别机制
Lakshmi P Jayanthi,Nahren Manuel Mascarenhas,Shachi Gosavi
Lakshmi P Jayanthi
Two mechanisms, induced fit (IF) and conformational selection (CS), have been proposed to explain ligand recognition coupled conformational changes. The histidine binding protein (HisJ) adopts the CS mechanism, in which a pre-equilibrium is...
Current limits of structural biology: The transient interaction between cytochrome c 6 and photosystem I [0.03%]
结构生物学的当前局限性——细胞色素c6与光系统I之间瞬态相互作用的例子
A Kölsch,C Radon,M Golub et al.
A Kölsch et al.
Trimeric photosystem I from the cyanobacterium Thermosynechococcus elongatus (TePSI) is an intrinsic membrane protein, which converts solar energy into electrical energy by oxidizing the soluble redox mediator cytochrome c 6 (Cyt c 6 ) and ...
Methods, development and applications of small-angle X-ray scattering to characterize biological macromolecules in solution [0.03%]
溶液中生物大分子小角X射线散射表征方法、技术及应用研究进展
Stefano Da Vela,Dmitri I Svergun
Stefano Da Vela
Applications of small-angle X-ray scattering (SAXS) in structural biology are reviewed. A brief introduction of the SAXS basics is followed by the presentation of the structural features of biological macromolecules in solution that can be ...
The variation among sites of protein structure divergence is shaped by mutation and scaled by selection [0.03%]
变异和选择影响蛋白质结构分歧在位点间的差异性分布
María Laura Marcos,Julian Echave
María Laura Marcos
Protein structures do not evolve uniformly, but the degree of structure divergence varies among sites. The resulting site-dependent structure divergence patterns emerge from a process that involves mutation and selection, which may both, in...