Rewards of divergence in sequences, 3-D structures and dynamics of yeast and human spliceosome SF3b complexes [0.03%]
酵母和人类剪接体SF3b复合物的序列、三维结构和动力学多样性及其意义
Arangasamy Yazhini,Sankaran Sandhya,Narayanaswamy Srinivasan
Arangasamy Yazhini
The evolution of homologous and functionally equivalent multiprotein assemblies is intriguing considering sequence divergence of constituent proteins. Here, we studied the implications of protein sequence divergence on the structure, dynami...
Jinan Wang,Lan Lan,Xiaoqing Wu et al.
Jinan Wang et al.
The Musashi RNA-binding proteins (RBPs) regulate translation of target mRNAs and maintenance of cell stemness and tumorigenesis. Musashi-1 (MSI1), long considered as an intestinal and neural stem cell marker, has been more recently found to...
Super-resolution confocal cryo-CLEM with cryo-FIB milling for in situ imaging of Deinococcus radiodurans [0.03%]
用于辐射耐受菌Deinococcus radiodurans就地成像的超分辨率共聚焦冷冻CLEM和冷冻FIB切削技术
Danielle L Sexton,Steffen Burgold,Andreas Schertel et al.
Danielle L Sexton et al.
Studying bacterial cell envelope architecture with electron microscopy is challenging due to the poor preservation of microbial ultrastructure with traditional methods. Here, we established and validated a super-resolution cryo-correlative ...
Allosteric regulation of binding specificity of HVEM for CD160 and BTLA ligands upon G89F mutation [0.03%]
G89F突变通过别构调节作用改变HVEM与CD160和BTLA配体的结合特异性
Rojan Shrestha,Sarah Garrett-Thomson,Weifeng Liu et al.
Rojan Shrestha et al.
Molecular interactions mediated by engagement of the Herpes virus entry mediator (HVEM) with members of TNF and Ig superfamily generate distinct signals in T cell activation pathways that modulate inflammatory and inhibitory responses. HVEM...
Replacement of heme by soluble guanylate cyclase (sGC) activators abolishes heme-nitric oxide/oxygen (H-NOX) domain structural plasticity [0.03%]
可溶性鸟苷酸环化酶(sGC)激活剂替代血红素抑制血红素-一氧化氮/氧气(H-NOX)结构域的结构可塑性
Aikaterini I Argyriou,Garyfallia I Makrynitsa,Georgios Dalkas et al.
Aikaterini I Argyriou et al.
The gasotransmitter nitric oxide (NO) is a critical endogenous regulator of homeostasis, in major part via the generation of cGMP (cyclic guanosine monophosphate) from GTP (guanosine triphosphate) by NO's main physiological receptor, the so...
The phosphatidylglycerol phosphate synthase PgsA utilizes a trifurcated amphipathic cavity for catalysis at the membrane-cytosol interface [0.03%]
磷脂甘油磷酸合成酶PgsA利用三分支两亲性空腔在膜-细胞质界面催化的机制
Bowei Yang,Hebang Yao,Dianfan Li et al.
Bowei Yang et al.
Phosphatidylglycerol is a crucial phospholipid found ubiquitously in biological membranes of prokaryotic and eukaryotic cells. The phosphatidylglycerol phosphate (PGP) synthase (PgsA), a membrane-embedded enzyme, catalyzes the primary react...
A rigorous framework for detecting SARS-CoV-2 spike protein mutational ensemble from genomic and structural features [0.03%]
从基因组和结构特征中检测SARS-CoV-2刺突蛋白突变集合的严格框架
Saman Fatihi,Surabhi Rathore,Ankit K Pathak et al.
Saman Fatihi et al.
The recent release of SARS-CoV-2 genomic data from several countries has provided clues into the potential antigenic drift of the coronavirus population. In particular, the genomic instability observed in the spike protein necessitates imme...
Role of actin cytoskeleton in the organization and function of ionotropic glutamate receptors [0.03%]
肌动蛋白细胞骨架在离子型谷氨酸受体的组织和功能中所起的作用
Priyanka Dutta,Pratibha Bharti,Janesh Kumar et al.
Priyanka Dutta et al.
Neural networks with precise connection are compulsory for learning and memory. Various cellular events occur during the genesis of dendritic spines to their maturation, synapse formation, stabilization of the synapse, and proper signal tra...
Structural insights into kinetoplastid coronin oligomerization domain and F-actin interaction [0.03%]
锥虫冠状素寡聚化结构域和F肌动蛋白相互作用的结构研究
Pankaj Singh Parihar,Aastha Singh,Sharanbasappa Shrimant Karade et al.
Pankaj Singh Parihar et al.
The two-domain actin associated protein coronin interacts with filamentous (F-) actin, facilitating diverse biological processes including cell proliferation, motility, phagocytosis, host-parasite interaction and cargo binding. The conserve...
A hierarchy of coupling free energies underlie the thermodynamic and functional architecture of protein structures [0.03%]
蛋白质结构的热力学和功能架构下的耦合自由能层次结构
Athi N Naganathan,Adithi Kannan
Athi N Naganathan
Protein sequences and structures evolve by satisfying varied physical and biochemical constraints. This multi-level selection is enabled not just by the patterning of amino acids on the sequence, but also via coupling between residues in th...