TISSUE CLEARING [0.03%]
组织透明化技术
Douglas S Richardson,Webster Guan,Katsuhiko Matsumoto et al.
Douglas S Richardson et al.
Tissue clearing of gross anatomical samples was first described over a century ago and has only recently found widespread use in the field of microscopy. This renaissance has been driven by the application of modern knowledge of optical phy...
Carlos J Bustamante,Yann R Chemla,Shixin Liu et al.
Carlos J Bustamante et al.
Optical tweezers have become the method of choice in single-molecule manipulation studies. In this Primer, we first review the physical principles of optical tweezers and the characteristics that make them a powerful tool to investigate sin...
Bioorthogonal chemistry [0.03%]
生物正交化学
Samuel L Scinto,Didier A Bilodeau,Robert Hincapie et al.
Samuel L Scinto et al.
Bioorthogonal chemistry represents a class of high-yielding chemical reactions that proceed rapidly and selectively in biological environments without side reactions towards endogenous functional groups. Rooted in the principles of physical...
Subcellular proteomics [0.03%]
亚细胞蛋白质组学
Josie A Christopher,Charlotte Stadler,Claire E Martin et al.
Josie A Christopher et al.
The eukaryotic cell is compartmentalized into subcellular niches, including membrane-bound and membrane-less organelles. Proteins localize to these niches to fulfil their function, enabling discreet biological processes to occur in synchron...
Bernd Reif,Sharon E Ashbrook,Lyndon Emsley et al.
Bernd Reif et al.
Solid-state nuclear magnetic resonance (NMR) spectroscopy is an atomic-level method used to determine the chemical structure, three-dimensional structure, and dynamics of solids and semi-solids. This Primer summarizes the basic principles o...