Mickaël Lelek,Melina T Gyparaki,Gerti Beliu et al.
Mickaël Lelek et al.
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at th...
Timothy M Wannier,Peter N Ciaccia,Andrew D Ellington et al.
Timothy M Wannier et al.
Recombination-mediated genetic engineering, also known as recombineering, is the genomic incorporation of homologous single-stranded or double-stranded DNA into bacterial genomes. Recombineering and its derivative methods have radically imp...
Bárbara B Mendes,João Conniot,Aviram Avital et al.
Bárbara B Mendes et al.
There is growing need for a safe, efficient, specific and non-pathogenic means for delivery of gene therapy materials. Nanomaterials for nucleic acid delivery offer an unprecedented opportunity to overcome these drawbacks; owing to their tu...
Fabrizio Torricelli,Demetra Z Adrahtas,Zhenan Bao et al.
Fabrizio Torricelli et al.
Electrolyte-gated transistors (EGTs), capable of transducing biological and biochemical inputs into amplified electronic signals and stably operating in aqueous environments, have emerged as fundamental building blocks in bioelectronics. In...
Karen M Hampson,Raphaël Turcotte,Donald T Miller et al.
Karen M Hampson et al.
Adaptive optics (AO) is a technique that corrects for optical aberrations. It was originally proposed to correct for the blurring effect of atmospheric turbulence on images in ground-based telescopes and was instrumental in the work that re...
TISSUE CLEARING [0.03%]
组织透明化技术
Douglas S Richardson,Webster Guan,Katsuhiko Matsumoto et al.
Douglas S Richardson et al.
Tissue clearing of gross anatomical samples was first described over a century ago and has only recently found widespread use in the field of microscopy. This renaissance has been driven by the application of modern knowledge of optical phy...
Carlos J Bustamante,Yann R Chemla,Shixin Liu et al.
Carlos J Bustamante et al.
Optical tweezers have become the method of choice in single-molecule manipulation studies. In this Primer, we first review the physical principles of optical tweezers and the characteristics that make them a powerful tool to investigate sin...
Bioorthogonal chemistry [0.03%]
生物正交化学
Samuel L Scinto,Didier A Bilodeau,Robert Hincapie et al.
Samuel L Scinto et al.
Bioorthogonal chemistry represents a class of high-yielding chemical reactions that proceed rapidly and selectively in biological environments without side reactions towards endogenous functional groups. Rooted in the principles of physical...
Subcellular proteomics [0.03%]
亚细胞蛋白质组学
Josie A Christopher,Charlotte Stadler,Claire E Martin et al.
Josie A Christopher et al.
The eukaryotic cell is compartmentalized into subcellular niches, including membrane-bound and membrane-less organelles. Proteins localize to these niches to fulfil their function, enabling discreet biological processes to occur in synchron...
Bernd Reif,Sharon E Ashbrook,Lyndon Emsley et al.
Bernd Reif et al.
Solid-state nuclear magnetic resonance (NMR) spectroscopy is an atomic-level method used to determine the chemical structure, three-dimensional structure, and dynamics of solids and semi-solids. This Primer summarizes the basic principles o...