Persistent gaps and errors in reference databases impede ecologically meaningful taxonomy assignments in 18S rRNA studies: a case study of terrestrial and marine nematodes [0.03%]
参考数据库中的持续差距和错误阻碍了18S rRNA研究中生态意义的分类赋值：陆地和海洋线虫的情况分析
Alejandro De Santiago,Tiago José Pereira,Timothy John Ferrero et al.
Alejandro De Santiago et al.
In metabarcoding studies, Linnaean taxonomy assignments of Operational Taxonomic Units (OTUs) or Amplicon Sequence Variants (ASVs) underpin many downstream bioinformatics analyses and ecological interpretations of environmental DNA (eDNA) d...
Eric D Stein,Christopher L Jerde,Elizabeth Andruszkiewicz Allan et al.
Eric D Stein et al.
The economic and methodological efficiencies of environmental DNA (eDNA) based survey approaches provide an unprecedented opportunity to assess and monitor aquatic environments. However, instances of inadequate communication from the scient...
rCRUX: A Rapid and Versatile Tool for Generating Metabarcoding Reference libraries in R [0.03%]
rCRUX：一种在R中生成 metabarcoding参考库的快速通用工具
Emily E Curd,Luna Gal,Ramon Gallego et al.
Emily E Curd et al.
The sequencing revolution requires accurate taxonomic classification of DNA sequences. Key to making accurate taxonomic assignments are curated, comprehensive reference barcode databases. However, the generation and curation of such databas...
John A Darling,Christopher L Jerde,Adam J Sepulveda
John A Darling
Misunderstandings regarding the term "false positive" present a significant hurdle to broad adoption of eDNA monitoring methods. Here, we identify three challenges to clear communication of false-positive error between scientists, managers,...