Metagenomic CRISPR Array Analysis Tool: a novel graph-based approach to finding CRISPR arrays in metagenomic datasets [0.03%]
基于图的从宏基因组数据集中寻找CRISPR列的新方法及其工具软件
Fikrat Talibli,Björn Voß
Fikrat Talibli
Clustered Regularly Interspersed Short Palindromic Repeats and CRISPR-associated genes (CRISPR-Cas) is a bacterial immune system also famous for its use in genome editing. The diversity of known systems could be significantly increased by m...
Cas9-independent tracrRNA cytotoxicity in Lacticaseibacillus paracasei [0.03%]
乳酸副癢菌中独立于Cas9的tracrRNA细胞毒性作用
Adini Q Arifah,Justin M Vento,Isabella Kurrer et al.
Adini Q Arifah et al.
CRISPR-Cas9 systems are widely used for bacterial genome editing, yet their heterologous expression has been associated with cytotoxicity. The Cas9 nuclease from Streptococcus pyogenes (SpyCas9) has been one common source, with reports of c...
CRISPR-Cas induced self-targeting identifies key players in archaeal microhomology-mediated end joining [0.03%]
基于CRISPR-Cas的自身靶向鉴定古菌微同源介导的非同源末端连接修复中关键蛋白
Anna-Lena Sailer,Julia Wörtz,Victoria Smith et al.
Anna-Lena Sailer et al.
DNA repair processes are the foundation for genome integrity and survival, especially in extreme environments where DNA damage occurs more frequently and where archaea are found. Nevertheless, first-hand experimental information on repair p...
Microbes are life, the biological core of One Health and Health in All Policies [0.03%]
微生物是生命，“同一个健康”和“卫生事务人人有责”的生物学核心
Kenneth Timmis,Fernando Baquero,James K Timmis et al.
Kenneth Timmis et al.
Editorial
microLife. 2025 Jun 28:6:uqaf012. DOI:10.1093/femsml/uqaf012 2025
Fernando Clavijo-Coppens,Deborah M Crepin,Héloïse Croizet et al.
Fernando Clavijo-Coppens et al.
This meeting report summarizes the scientific activities of the ninth annual conference of Phages.fr, organized by the French Phages network. This year, the conference took place from 12 to 14 November 2024, in Sète, in the south of France...
Lena Mitousis,Ewa Musiol-Kroll,Wolfgang Wohlleben
Lena Mitousis
Actinomycetes are important producers of valuable natural products that are applied in medicine or industry. The enzymes necessary for the synthesis of those compounds are encoded in biosynthetic gene clusters (BGCs) in the genome. However,...
Heterodimerization of staphylococcal phage φ2638A endolysin isoforms and their functional role in bacterial lysis [0.03%]
金黄色葡萄球菌噬菌体φ2638A 溶菌酶异二聚化及其在细菌裂解中的功能作用
Léa V Zinsli,Anna M Sobieraj,Jiemin Du et al.
Léa V Zinsli et al.
Bacteriophage endolysins targeting Gram-positive bacteria typically feature a modular architecture of one or more enzymatically active domains (EADs) and cell wall binding domains (CBDs). Several endolysins also feature internal translation...
Metabolic pathway analysis of the methylcitrate cycle in bacteria and fungi identifies methylcitrate synthase as an antiinfective drug target [0.03%]
甲基柠檬酸循环的代谢途径分析：细菌和真菌中的甲基柠檬酸合成酶是抗菌药物靶点
Lukas Korn,Matthias Brock,Stefan Schuster
Lukas Korn
The tricarboxylic acid (TCA) cycle is well known as a crucial pathway in central metabolism in many organisms. A less known analogous pathway is the methylcitrate cycle (MCC). It is present in various fungi such as Aspergillus species and b...
Respiratory syncytial virus: health burden, disease prevention, and treatment-recent progress and lessons learned [0.03%]
呼吸道合胞病毒：健康负担、疾病预防和治疗——近期进展与教训
Harald Brüssow
Harald Brüssow
Respiratory syncytial virus (RSV), a negative-sense single-stranded RNA virus of the Pneumoviridae family, represents the most important pathogen of lower respiratory tract infections in young infants causing yearly epidemics. RSV is also a...
Provirus deletion from Haloferax volcanii affects motility, stress resistance, and CRISPR RNA expression [0.03%]
霍沃火山球菌中的前病毒缺失影响运动性、胁迫抗性和CRISPR RNA表达
Nadia Di Cianni,Simon Bolsinger,Jutta Brendel et al.
Nadia Di Cianni et al.
Haloferax volcanii harbours four putative proviruses: Halfvol1, Halfvol2, Halfvol3, and Halfvol4. In this study, we successfully deleted all four provirus genomes, demonstrating, that they are not essential. Transcriptome comparison between...