A modular genetic toolbox for precise gene regulation and multi-color imaging in streptococci [0.03%]
用于链球菌精确基因调控和多色成像的模块化遗传工具箱
Johann Mignolet,Albane Schmid,Johan Staub et al.
Johann Mignolet et al.
Fluorescent labeling is a powerful tool in microbiology allowing live cell imaging and providing insights into dynamic cellular processes, quantification of gene expression, and protein subcellular localization. Although multicolor imaging ...
Proteome characterization of single gene deletion mutants lacking zinc finger µ-proteins in Haloferax volcanii [0.03%]
无锌指µ蛋白的单基因缺失突变体的蛋白质组特征分析研究——以Haloferax volcanii为例
Pedro H C Franco,Deniz Üresin,Andreas Borst et al.
Pedro H C Franco et al.
Microproteins (≤70 amino acids) have important and often essential roles in all kingdoms of life, influencing cell motility, regulation of membrane transport and as transcription factors. In the halophilic archaeon and model system Halofer...
Anna Möslinger,Stefanie Allert,Slavena Vylkova et al.
Anna Möslinger et al.
Modern medical advances have contributed to a growing population of immunocompromised individuals, increasing susceptibility to human fungal pathogens. The leading species for invasive fungal diseases are Candida albicans and Aspergillus fu...
Caspofungin enhances antifungal immunity in human blood through transcriptional reprogramming [0.03%]
卡泊芬净通过转录重构增强人类血液的抗真菌免疫力
Aia Shehata,Sascha Schäuble,Ronny Martin et al.
Aia Shehata et al.
Candida albicans is a leading cause of invasive candidiasis, a life-threatening infection with high mortality despite antifungal therapy. Echinocandins are first-line agents due to their fungicidal activity and low toxicity profile. Beyond ...
Impact of SliP4 deletion on the high-light acclimation in Synechocystis sp. PCC 6803 [0.03%]
SliP4缺失对集胞藻6803株系高光适应的影响
Luna Alvarenga-Lucius,Sandra Maaß,Viktoria Reimann et al.
Luna Alvarenga-Lucius et al.
SliP4 is a small, 37 amino acids protein that is strongly induced when the cyanobacterium Synechocystis sp. PCC 6803 is exposed to high-light (HL) conditions. Deletion mutants manifest a light-sensitive phenotype due to impaired cyclic elec...
Less missing values-evaluation of proteomics workflows for the quantification of (small) proteins [0.03%]
更少的缺失值：用于量化（小型）蛋白质的工作流程的评估
Jürgen Bartel,Vaikhari Kale,Dennis Joshua Pyper et al.
Jürgen Bartel et al.
Quantitative information on protein abundance is crucial to understand biological processes and is therefore frequently gathered in proteomic studies. However, the quality of a quantitative proteomic dataset is greatly affected by the numbe...
Enhanced ADP-heptose-dependent NF-κB activation by Helicobacter pylori CagA through cortactin-Src-dependent tyrosine phosphorylation of IKKβ [0.03%]
幽门螺旋杆菌CagA通过cortactin-Src依赖的IKKβ酪氨酸磷酸化增强ADP-heptose依赖性NF-κB活化作用
Irshad Sharafutdinov,Nicole Tegtmeyer,Barbara Friedrich et al.
Irshad Sharafutdinov et al.
Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) represents a family of important transcription factors in innate immunity. We have previously reported that the gastric pathogen Helicobacter pylori needs the actin-bin...
Comprehensive analysis of CRISPR array repeat mutations reveals subtype-specific patterns and links to spacer dynamics [0.03%]
对CRISPR数组重复序列突变的综合分析揭示了亚型特异性的模式及其与spacer动态变化之间的联系
Alexander Mitrofanov,Chase L Beisel,Franz Baumdicker et al.
Alexander Mitrofanov et al.
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and their associated CRISPR-associated protein (Cas) systems are adaptive immune mechanisms in bacteria and archaea that protect against invading genetic elements by integra...
Manon Ferry,Connor Sharp,Isabelle J Schalk et al.
Manon Ferry et al.
Salmonella enterica, a prominent enteric pathogen, employs sophisticated iron acquisition mechanisms to overcome host-imposed iron limitation, notably through the production and uptake of siderophores-small, high-affinity iron-chelating com...
CRISPR-Cas targeting in Haloferax volcanii promotes within-species gene exchange by triggering homologous recombination [0.03%]
基于CRISPR-Cas的基因编辑可引发极端嗜盐古菌基因内交换的新机制研究
Deepak Kumar Choudhary,Israela Turgeman-Grott,Shachar Robinzon et al.
Deepak Kumar Choudhary et al.
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (CRISPR-associated genes) systems provide adaptive immunity in bacteria and archaea against mobile genetic elements, but the role they play in gene exchange and speciati...