Emily R Smith,Dua H Al-Smadi,Yitao Dai et al.
Emily R Smith et al.
Well-recognized for the ability to modulate spliceosome activity, the pladienolide family of polyketide natural products has been the recent subject of intense synthetic and bioactivity studies. However, our understanding of their biosynthe...
RaPID discovery of cell-permeable helical peptide inhibitors con-taining cyclic β-amino acids against SARS-CoV-2 main protease [0.03%]
含有环状β-氨基酸的细胞可渗透螺旋肽抑制剂对SARS-CoV-2主要蛋白酶的RaPID发现
Marina Kawai,Tika R Malla,H T Henry Chan et al.
Marina Kawai et al.
Structurally constrained cyclic β-amino acids are attractive building blocks for peptide drugs because they induce unique and stable conformations. Introduction of (1S,2S)-2-aminocyclopentanecarboxylic acid [(1S,2S)-2-ACPC] into peptides s...
Mechanistic insights into the ATP-mediated and species-dependent inhibition of TrpRS by chuangxinmycin [0.03%]
昌新霉素对色氨酸合成酶的物种特异性及ATP介导的抑制作用机制研究
Yichen Ren,Sili Wang,Wen Liu et al.
Yichen Ren et al.
Chuangxinmycin (CXM) is a promising antimicrobial compound targeting bacterial tryptophanyl-tRNA synthetase (TrpRS), an essential enzyme in protein synthesis. The detailed inhibitory mechanism of CXM, particularly in clinically relevant pat...
Laura Hayward,Matthias G J Baud
Laura Hayward
Cysteine sulfinic acid (CSA) is a stable post translational modification in nature. While long considered to be an irreversible by-product of accidental overoxidation of the cysteine sulfur, evidence in the last two decades has accumulated ...
Turn-on fluorescent glucose transport bioprobe enables wash-free real-time monitoring of glucose uptake activity in live cells and small organisms [0.03%]
开启型荧光葡萄糖转运生物探针能实时检测活细胞和小型模式动物中葡萄糖的摄入情况
Monica S Hensley,David Hutchings,Aldelrahman Ismail et al.
Monica S Hensley et al.
The direct link between sugar uptake and metabolic diseases highlights the iminent need for molecular tools to detect and evaluate alterations in sugar uptake efficiency as approaches to identify disease-relevant metabolic alterations. Howe...
Exploring sequence- and structure-based fitness landscapes to enhance thermal resistance and activity of endoglucanase II with minimal experimental effort [0.03%]
基于序列和结构的适应度景观探索，以最小的实验努力增强内切葡萄糖甘酶II的耐热性和活性
Atul Kumar,Alexander-Maurice Illig,Nicolas de la Vega Guerra et al.
Atul Kumar et al.
Enhancing the performance of cellulases at high temperatures is crucial for efficient biomass hydrolysis-a fundamental process in biorefineries. Traditional protein engineering methods, while effective, are time-consuming and labour-intensi...
Felicity J Frank,Rebecca A Lawson,Tom E McAllister
Felicity J Frank
Protein glycosylation is one of the most abundant and complex post-translational modifications, necessitating many different approaches to fully understand the biological effects. Investigation using synthetic glycopeptides is limited by th...
Design of a stapled peptide that binds to the Ebola virus matrix protein dimer interface [0.03%]
一种与埃博拉病毒基质蛋白二聚体界面结合的环肽的设计
Roopashi Saxena,Madison M Wright,Benjamin M Rathman et al.
Roopashi Saxena et al.
The Ebola virus (EBOV) is a filamentous lipid-enveloped RNA virus that can cause viral hemmorhagic fever and has a high fatility rate. EBOV encodes seven genes including the lipid-binding matrix protein, VP40, which lies beneath the lipid-e...
Co-delivery of antigen and adjuvant by site-specific conjugation to dendritic cell-targeted Fab fragments potentiates T cell responses [0.03%]
特定位点连接到树突状细胞靶向Fab片段的抗原和佐剂共递送可增强T细胞反应
Zacharias Wijfjes,Iván Ramos Tomillero,Camille M Le Gall et al.
Zacharias Wijfjes et al.
The aim of therapeutic cancer vaccines is to induce tumor-specific cellular immune responses. This requires tumor antigens to be efficiently processed and presented by antigen-presenting cells, in particular dendritic cells (DCs). In additi...
Monitoring SARS-CoV-2 Nsp13 helicase binding activity using expanded genetic code techniques [0.03%]
利用扩增遗传密码技术监测SARS-CoV-2 Nsp13解旋酶的结合活性
Eryn Lundrigan,Christine Hum,Nadine Ahmed et al.
Eryn Lundrigan et al.
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) non-structural protein 13 (Nsp13) helicase is a multi-functional protein that can unwind dsDNA and dsRNA in an NTP-dependent manner. Given that this viral helicase is essentia...