Fluorescence lifetime predicts performance of voltage sensitive fluorophores in cardiomyocytes and neurons [0.03%]
荧光寿命预测电压敏感性荧光染料在心肌细胞和神经元中的性能
Steven C Boggess,Julia R Lazzari-Dean,Benjamin K Raliski et al.
Steven C Boggess et al.
Voltage imaging with fluorescent indicators offers a powerful complement to traditional electrode or Ca2+-imaging approaches for monitoring electrical activity. Small molecule fluorescent indicators present the unique opportunity for exquis...
The two domains of human galectin-8 bind sialyl- and fucose-containing oligosaccharides in an independent manner. A 3D view by using NMR [0.03%]
人galectin-8的两个功能区独立地结合唾液酸和岩藻糖寡糖:NMR三维观察结果
Marcos Gómez-Redondo,Sandra Delgado,Reyes Núñez-Franco et al.
Marcos Gómez-Redondo et al.
The interaction of human galectin-8 and its two separate N-terminal and C-terminal carbohydrate recognition domains (CRD) to their natural ligands has been analysed using a synergistic combination of experimental NMR and ITC methods, and mo...
A bicyclic S-adenosylmethionine regeneration system applicable with different nucleosides or nucleotides as cofactor building blocks [0.03%]
一种适用于不同核苷或碱基作为辅助因子构建块的环形S-腺苷甲硫氨酸再生系统
Désirée Popadić,Dipali Mhaindarkar,Mike H N Dang Thai et al.
Désirée Popadić et al.
The ubiquitous cofactor S-adenosyl-l-methionine (SAM) is part of numerous biochemical reactions in metabolism, epigenetics, and cancer development. As methylation usually improves physiochemical properties of compounds relevant for pharmace...
Synthesis and immunological effects of C14-linked 4,5-epoxymorphinan analogues as novel heroin vaccine haptens [0.03%]
一类新型海洛因疫苗半抗原的合成及其免疫学效应研究
Eugene S Gutman,Thomas C Irvin,J Brian Morgan et al.
Eugene S Gutman et al.
Active immunization is being explored as a potential therapeutic to combat accidental overdose and to mitigate the abuse potential of opioids. Hapten design is one of the crucial factors that determines the efficacy of a candidate vaccine t...
Investigation of the biological functions of heparan sulfate using a chemoenzymatic synthetic approach [0.03%]
采用化学酶法合成方法研究硫酸肝素的生物学功能
Zhangjie Wang,Katelyn Arnold,Vijay Manohar Dhurandhare et al.
Zhangjie Wang et al.
Heparan sulfate (HS) is a highly sulfated polysaccharide playing essential physiological and pathophysiological roles in the animal kingdom. Heparin, a highly sulfated form of HS, is a widely used anticoagulant drug. Isolated from biologica...
In vivo modulation of ubiquitin chains by N-methylated non-proteinogenic cyclic peptides [0.03%]
用于调节泛素链的N-甲基非蛋白质环形肽在体内的修饰作用
Joseph M Rogers,Mickal Nawatha,Betsegaw Lemma et al.
Joseph M Rogers et al.
Cancer and other disease states can change the landscape of proteins post-translationally tagged with ubiquitin (Ub) chains. Molecules capable of modulating Ub chains are potential therapeutic agents, but their discovery represents a signif...
Development and biological applications of sulfur-triazole exchange (SuTEx) chemistry [0.03%]
用于生物正交反应的硫(芳基)三唑交换(SuTEx)化学及其应用
Adam L Borne,Jeffrey W Brulet,Kun Yuan et al.
Adam L Borne et al.
Sulfur electrophiles constitute an important class of covalent small molecules that have found widespread applications in synthetic chemistry and chemical biology. Various electrophilic scaffolds, including sulfonyl fluorides and arylfluoro...
Light-induced modulation of DNA recognition by the Rad4/XPC damage sensor protein [0.03%]
光诱导的Rad4 / XPC损伤传感器蛋白对DNA识别的调节作用
Amirrasoul Tavakoli,Debamita Paul,Hong Mu et al.
Amirrasoul Tavakoli et al.
Biomolecular structural changes upon binding/unbinding are key to their functions. However, characterization of such dynamical processes is difficult as it requires ways to rapidly and specifically trigger the assembly/disassembly as well a...
Bypassing the requirement for aminoacyl-tRNA by a cyclodipeptide synthase enzyme [0.03%]
一种cyclodipeptide合成酶通过旁路氨基酰基tRNA要求来发挥作用
Christopher J Harding,Emmajay Sutherland,Jane G Hanna et al.
Christopher J Harding et al.
Cyclodipeptide synthases (CDPSs) produce a variety of cyclic dipeptide products by utilising two aminoacylated tRNA substrates. We sought to investigate the minimal requirements for substrate usage in this class of enzymes as the relationsh...
The pursuit of mechanism of action: uncovering drug complexity in TB drug discovery [0.03%]
作用机制的研究：结核病药物发现中的药物复杂性探究
Tianao Yuan,Joshua M Werman,Nicole S Sampson
Tianao Yuan
Whole cell-based phenotypic screens have become the primary mode of hit generation in tuberculosis (TB) drug discovery during the last two decades. Different drug screening models have been developed to mirror the complexity of TB disease i...