Bypassing the requirement for aminoacyl-tRNA by a cyclodipeptide synthase enzyme [0.03%]
一种cyclodipeptide合成酶通过旁路氨基酰基tRNA要求来发挥作用
Christopher J Harding,Emmajay Sutherland,Jane G Hanna et al.
Christopher J Harding et al.
Cyclodipeptide synthases (CDPSs) produce a variety of cyclic dipeptide products by utilising two aminoacylated tRNA substrates. We sought to investigate the minimal requirements for substrate usage in this class of enzymes as the relationsh...
The pursuit of mechanism of action: uncovering drug complexity in TB drug discovery [0.03%]
作用机制的研究：结核病药物发现中的药物复杂性探究
Tianao Yuan,Joshua M Werman,Nicole S Sampson
Tianao Yuan
Whole cell-based phenotypic screens have become the primary mode of hit generation in tuberculosis (TB) drug discovery during the last two decades. Different drug screening models have been developed to mirror the complexity of TB disease i...
Modified nucleoside triphosphates in bacterial research for in vitro and live-cell applications [0.03%]
用于体外和活细胞应用的细菌研究中的改性核苷三磷酸
Adeline Espinasse,Hannah K Lembke,Angela A Cao et al.
Adeline Espinasse et al.
Modified nucleoside triphosphates (NTPs) are invaluable tools to probe bacterial enzymatic mechanisms, develop novel genetic material, and engineer drugs and proteins with new functionalities. Although the impact of nucleobase alterations h...
A live-cell assay for the detection of pre-microRNA-protein interactions [0.03%]
一种用于检测前体microRNA-蛋白相互作用的活细胞测定法
Sydney L Rosenblum,Daniel A Lorenz,Amanda L Garner
Sydney L Rosenblum
Recent efforts in genome-wide sequencing and proteomics have revealed the fundamental roles that RNA-binding proteins (RBPs) play in the life cycle and function of coding and non-coding RNAs. While these methodologies provide a systems-leve...
Long-range PEG Stapling: Macrocyclization for Increased Protein Conformational Stability and Resistance to Proteolysis [0.03%]
长程PEG接枝环化增强蛋白质构象稳定性和抗蛋白酶降解性
Qiang Xiao,Dallin S Ashton,Zachary B Jones et al.
Qiang Xiao et al.
We previously showed that long-range stapling of two Asn-linked O-allyl PEG oligomers via olefin metathesis substantially increases the conformational stability of the WW domain through an entropic effect. The impact of stapling was more fa...
Natural product scaffolds as inspiration for the design and synthesis of 20S human proteasome inhibitors [0.03%]
天然产物结构作为设计和合成20S人体蛋白酶抑制剂的启发来源
Grace E Hubbell,Jetze J Tepe
Grace E Hubbell
The 20S proteasome is a valuable target for the treatment of a number of diseases including cancer, neurodegenerative disease, and parasitic infection. In an effort to discover novel inhibitors of the 20S proteasome, many reseaarchers have ...
Dynamic visualization of type II peptidyl carrier protein recognition in pyoluteorin biosynthesis [0.03%]
角黄素生物合成中动态可视化II型肽载体蛋白识别过程
Joshua C Corpuz,Larissa M Podust,Tony D Davis et al.
Joshua C Corpuz et al.
Using a covalent chemical probe and X-ray crystallography coupled to nuclear magnetic resonance data, we elucidated the dynamic molecular basis of protein recognition between the carrier protein and adenylation domain in pyoluteorin biosynt...
Biological Evaluation of Native Streptococcal Competence Stimulating Peptides Reveal Potential Crosstalk Between Streptococcus mitis and Streptococcus pneumoniae and a New Scaffold for the Development of S. pneumoniae Quorum Sensing Modulators [0.03%]
天然链球菌刺激肽的生物评价揭示了口腔链球菌和肺炎链球菌之间可能存在交流，及用于开发肺炎链球菌群体感应调节剂的新框架
Tahmina Ahmed Milly,Yftah Tal-Gan
Tahmina Ahmed Milly
Streptococcus pneumoniae, an opportunistic human pathogen, acquires genes from its neighboring species of the mitis group of streptococci that confer antibiotic resistances and allow it to produce diverse virulence factors. Most species of ...
Unravelling the role of key amino acid residues of the parainfluenza fusion peptide in membrane fusion [0.03%]
解开融合肽上几个关键氨基酸残基在副流感病毒膜融合过程中的作用
Parainfluenza viruses enter host cells by fusing their envelope with the cell membrane. In this process mediated by the fusion glycoprotein, the fusion peptide plays an essential role in membrane binding and triggering fusion. Previously, w...