Identifying cysteine residues susceptible to oxidation by photoactivatable atomic oxygen precursors using a proteome-wide analysis [0.03%]
采用基于蛋白质组的方法鉴定可被光激活原子氧前体氧化的半胱氨酸残基
Ankita Isor,Benjamin V Chartier,Masahiro Abo et al.
Ankita Isor et al.
The reactivity profile of atomic oxygen [O(3P)] in the condensed phase has shown a preference for the thiol group of cysteines. In this work, water-soluble O(3P)-precursors were synthesized by adding aromatic burdens and water-soluble sulph...
Hyaluronan (HA)-inspired glycopolymers as molecular tools for studying HA functions [0.03%]
以透明质酸（HA）为灵感的糖基聚合物作为研究HA功能的分子工具
Dominic W P Collis,Gokhan Yilmaz,Yichen Yuan et al.
Dominic W P Collis et al.
Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and prolif...
Metathramycin, a new bioactive aureolic acid discovered by heterologous expression of a metagenome derived biosynthetic pathway [0.03%]
由异源表达获得的新 aureolic 酸类天然产物代谢硫霉素
Luke J Stevenson,Joe Bracegirdle,Liwei Liu et al.
Luke J Stevenson et al.
Bacterial natural products have been a rich source of bioactive compounds for drug development, and advances in DNA sequencing, informatics and molecular biology have opened new avenues for their discovery. Here, we describe the isolation o...
Revision in the first steps of the biosynthesis of the red antibiotic prodigiosin: use of a synthetic thioester to validate a new intermediate [0.03%]
红抗生素角黄素生物合成第一步的修正：使用合成硫酯验证新的中间体
Maxime Couturier,Hiral D Bhalara,Rita E Monson et al.
Maxime Couturier et al.
A biosynthetic pathway for the red-antibiotic, prodigiosin, was proposed over a decade ago but not all the suggested intermediates could be detected experimentally. Here we show that a thioester that was not originally included in the pathw...
Site-specific modification and segmental isotope labelling of HMGN1 reveals long-range conformational perturbations caused by posttranslational modifications [0.03%]
HMGN1的位点特异性修饰及片段同位素标记揭示了翻译后修饰引起的长程构象变化
Gerhard Niederacher,Debra Urwin,Yasmin Dijkwel et al.
Gerhard Niederacher et al.
Interactions between histones, which package DNA in eukaryotes, and nuclear proteins such as the high mobility group nucleosome-binding protein HMGN1 are important for regulating access to DNA. HMGN1 is a highly charged and intrinsically di...
Short oligoalanine helical peptides for supramolecular nanopore assembly and protein cytosolic delivery [0.03%]
用于超分子纳米孔组装和蛋白质细胞质递送的短聚丙氨酸螺旋肽
Marta Pazo,Giulia Salluce,Irene Lostalé-Seijo et al.
Marta Pazo et al.
In this work we report a rational design strategy for the identification of new peptide prototypes for the non-disruptive supramolecular permeation of membranes and the transport of different macromolecular giant cargos. The approach target...
Rational design of a stapled JAZ9 peptide inhibiting protein-protein interaction of a plant transcription factor [0.03%]
合理设计抑制植物转录因子蛋白质相互作用的环肽JAZ9
Kaho Suzuki,Yousuke Takaoka,Minoru Ueda
Kaho Suzuki
We herein describe the development of a stapled peptide inhibitor for a jasmonate-related transcription factor. The designed peptide selectively inhibited MYCs, master-regulators of jasmonate signaling, and selectively suppressed MYC-mediat...
Metal transport mechanism of the cation diffusion facilitator (CDF) protein family - a structural perspective on human CDF (ZnT)-related diseases [0.03%]
锌转运蛋白ZnT的结构及其在金属离子转运障碍相关疾病中的作用研究
Shiran Barber-Zucker,Arie Moran,Raz Zarivach
Shiran Barber-Zucker
Divalent d-block metal cations (DDMCs) participate in many cellular functions; however, their accumulation in cells can be cytotoxic. The cation diffusion facilitator (CDF) family is a ubiquitous family of transmembrane DDMC exporters that ...
Dongsheng Bai,Jinying Peng,Chengqi Yi
Dongsheng Bai
Single-cell profiling methods are developed to dissect heterogeneity of cell populations. Recently, multiple enzymatic or chemical treatments have been integrated into single-cell multi-omics profiling methods with high compatibility. These...
Crosslinker-modified nucleic acid probes for improved target identification and biomarker detection [0.03%]
交联剂修饰的核酸探针可改善目标识别与生物标志物检测
Joke Elskens,Annemieke Madder
Joke Elskens
Understanding the intricate interaction pattern of nucleic acids with other molecules is essential to gain further insight in biological processes and disease mechanisms. To this end, a multitude of hybridization-based assays have been desi...