Application of the YebF secretion pathway in Escherichia coli for rapid, on-plate screening of PETase libraries for improved activity [0.03%]
利用耶巴克氏菌分泌途径在大肠杆菌中快速筛选PET酶文库以提高其活性
Jaeick Lee,Maame Yaa Yamoa,Celina L Bradley et al.
Jaeick Lee et al.
Plastic waste such as polyethylene terephthalate (PET) is a major environmental burden, and enzymes capable of degrading PET are emerging as biocatalytic tools for sustainable recycling. Progress in improving PET hydrolases (or PETases) has...
Recent progress in cGAS-STING agonist design and mechanisms of cancer immune modulation [0.03%]
cGAS-STING激动剂设计的最新进展及其在癌症免疫调节中的机制研究
Liao Wang,Muhammad Nafees,He Fei et al.
Liao Wang et al.
The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signalling pathway is a crucial modulator of innate immunity and an important target for next-generation cancer immunotherapy. Numerous cGAS-STING agonists have been ...
ortho-Substituents govern aryl aldehyde reactivity: toward lysine-targeted, tunable inhibitors of glucose-6-phosphate dehydrogenase [0.03%]
间位取代基调控苯甲醛反应活性：用于靶向赖氨酸的调变葡萄糖酸-6-磷酸脱氢酶抑制剂的设计与发展
Bronwyn E Rowland,Kelsey D Roy,Adil Alkaş et al.
Bronwyn E Rowland et al.
Certain drugs achieve highly potent and long-lasting enzyme inhibition by forming covalent bonds with nucleophilic amino acid functionalities. Aldehyde-containing inhibitors are able to react with lysine ε-amino functionalities to form rev...
Synthesis of thermostable artificial nicotinamide cofactors: carba-NAD+ and carba-NADP [0.03%]
耐热人工烟酰胺辅因子的合成：碳酰NAD+和碳酰NADP+
Zinnia Dsouza,Jan-Simon Jeshua Friedrichs,Manuel Döring et al.
Zinnia Dsouza et al.
Carbocyclic nicotinamide cofactors are attractive NAD(P)+ analogues that retain native redox activity while offering substantially enhanced chemical and thermal stability. Their broader use, however, has been limited by demanding multistep ...
Development of a spectrophotometric assay for high-throughput screening and mechanistic characterization of glucose-1-phosphate thymidylyltransferase inhibitors [0.03%]
葡萄糖-1-磷酸胸苷基转移酶抑制剂的高通量筛选及作用机制的分光光度测定法研究
Bronwyn E Rowland,Jesse C Fuller,Chigozie L Okolie et al.
Bronwyn E Rowland et al.
The sugar β-l-rhamnose is often a conserved component of bacterial polysaccharide capsules, an extracellular structure featured in many drug-resistant, pathogenic bacteria. The β-l-rhamnose biosynthetic enzymes (RmlA-D) have therefore bec...
Stability modification of therapeutic aptamers: from biostability bottlenecks to nuclease-resistant construct design [0.03%]
治疗性适配体的稳定性改进：从生物稳定性的瓶颈到抗核酸酶结构的设计
Zhuoheng Pan,Yufei Pan,Huarui Zhang et al.
Zhuoheng Pan et al.
Nucleic acid aptamers are synthetic, single-stranded oligonucleotides that bind targets with high affinity and specificity, enabling precise and programmable functional modulation. However, low biostability remains a key druggability bottle...
Native MS and ligand observed NMR uncovers subtle SLiM binding variations that mediate HSP90-Hop PPI modulation [0.03%]
天然MS和配体观察NMR揭示了细微的SLiM结合变化，这些变化介导HSP90-Hop PPI调节
Tara K Davids,Daniel A Kusza,Shannon K Misplon et al.
Tara K Davids et al.
We have previously demonstrated that a non-natural tetrazole containing peptide (2), which mimics the MEEVD Short Linear Motif (SLiM) found at the Heat Shock Protein 90 (HSP90) C-terminus, disrupts the transient protein-protein interaction ...
De novo acyl carrier proteins display structure-independent modification and sequence novelty [0.03%]
从头合成的乙酰载体蛋白显示结构独立修饰和序列新颖性
Michael A Herrera,Grace K King,Zoe Ozols et al.
Michael A Herrera et al.
Acyl carrier proteins (ACPs) are dynamic, structurally conserved α-helical proteins central to many primary and secondary metabolic processes. Whilst prior engineering efforts have focused on strategic mutagenesis and "helix swaps", much o...
Strategies to overcome hepatic clearance of endogenous proteins - molecular and formulation approaches [0.03%]
克服肝脏清除内源性蛋白质的策略-分子和制剂方法
Kirstin Meiners,Tobias Stepic,Lorenz Meinel et al.
Kirstin Meiners et al.
The medical use of biologics is often limited by rapid elimination from circulation. This review explores formulation strategies for enhancing the pharmacokinetic profiles of therapeutic proteins with hepatic clearance as a major route of e...
RNA-binding landscape of amiloride: large-scale profiling and structural basis of U-U mismatch recognition [0.03%]
氨基锂的RNA结合图谱：UU错配识别的大规模鉴定及其结构基础
Kosuke Tsuzuki,Kazumitsu Onizuka,Momo Okada et al.
Kosuke Tsuzuki et al.
Amiloride possesses a characteristic chemical scaffold capable of recognizing uracil (U) through three complementary hydrogen bonds; however, its binding selectivity toward naturally occurring RNA structural motifs has remained uncharacteri...