Reductively activated CPP-PROTAC nanocomplexes enhance target degradation via efficient cellular uptake [0.03%]
可还原激活的CPP- PROTAC纳米复合物通过高效细胞摄取增强目标降解
Maho Miyamoto,Kosuke Saito,Hidetomo Yokoo et al.
Maho Miyamoto et al.
We developed a nanoparticle based on a cell-penetrating peptide-PROTAC conjugate with a disulfide linker, MZ1-R9, and dextran sulfate, enhancing cellular uptake and BRD4 degradation. This delivery platform significantly improves PROTAC bioa...
Differential melting voltage by tandem-trapped ion mobility spectrometry: glycan structure influences glycoprotein stability [0.03%]
串联俘获离子淌度谱的差示熔化电压：糖结构影响糖蛋白稳定性
Mengqi Chai,Christian Bleiholder,Fanny C Liu
Mengqi Chai
Profiling the full spectrum of protein glycoforms is critical to understanding their functional roles. We developed the differential melting voltage approach using tandem-ion mobility/tandem-mass spectrometry and applied it to study Ribonuc...
Detection and characterisation of ligand-induced conformational changes in acetylcholine binding proteins using biosensors and X-ray crystallography [0.03%]
基于生物传感器和X射线晶体学的乙酰胆碱结合蛋白配体诱导构象变化的检测与表征
Edward A FitzGerald,Daniela Cederfelt,Daria Kovryzhenko et al.
Edward A FitzGerald et al.
Analysis of ligand-induced structural changes in proteins is challenging due to the lack of experimental methods suited for detection and characterisation of both ligand binding and induced structural changes. We have explored biosensors wi...
Characterization of nuclease stability and poly(A)-binding protein binding activity of chemically modified poly(A) tail for in vivo applications [0.03%]
化学修饰的聚腺苷酸(Poly(A))尾巴的核酸酶稳定性和多聚腺苷酸结合蛋白(poly(A)-bindingprotein，PABP)结合活性分析及其体内应用研究
Atsushi Hashimoto,Yuma Kunitomo,Ittoku Kikuchi et al.
Atsushi Hashimoto et al.
The poly(A) tail plays a crucial role in mRNA stability and translation efficiency. Chemical modification of the poly(A) tail is a promising approach for stabilizing mRNA against deadenylation. In this study, we investigated the effect of p...
Applications of click and click-to-release chemistry in biomaterials to advance skin regeneration [0.03%]
点击化学和“点击释放”化学在皮肤再生生物材料中的应用研究进展
Merel Gansevoort,Matthijs van de Waarsenburg,Thomas J Boltje et al.
Merel Gansevoort et al.
Achieving skin regeneration following destruction of the epidermis and dermis (e.g. full-thickness wounds) has remained an unachieved goal. The wound healing response is complex and consists of multiple overlapping phases which are tightly ...
Investigating the N-terminal linker histone H1 subtypes as substrates for JmjC lysine demethylases [0.03%]
探究N端连接组蛋白H1亚型作为JmjC赖氨酸脱甲基酶底物的功能
Vildan A Türkmen,Anthony Tumber,Eidarus Salah et al.
Vildan A Türkmen et al.
Members of the Jumonji C (JmjC) subfamily of non-heme Fe(ii) and 2-oxoglutarate (2OG) dependent N ε-lysine demethylases have established roles in catalysing demethylation of N ε-methylated lysine residues in core histones; their roles in ...
Copper-catalysed azide-alkyne cycloaddition on live M13 bacteriophage for expanding the molecular diversity of phage-displayed peptide libraries [0.03%]
用于扩大噬菌体肽展示文库分子多样性的活M13噬菌体上的铜催化叠氮炔基环加成反应
Olabode Dawodu,Cody A White,Caitlin Specht et al.
Olabode Dawodu et al.
Phage display is a powerful platform for ligand evolution, but conventional phage display libraries are confined to the twenty canonical amino acids, greatly limiting the chemical space that these libraries can be used to explore. Here we p...
Sheel C Dodani,Ariel Furst
Sheel C Dodani
As both chemical and biological engineering approaches continue to expand, the landscape of biomolecular technologies is rapidly evolving, affording new opportunities from basic science to real-world applications. This themed collection bri...
Editorial
RSC chemical biology. 2025 Aug 19. DOI:10.1039/d5cb90031j 2025
Michelle Marie B Helmeke,Rhianna L Haynie-Cion,Matthew R Pratt
Michelle Marie B Helmeke
Metabolic oligosaccharide engineering (MOE) is a transformative technology, enabling the chemical labeling and subsequent analysis of glycans. Central to this method are monosaccharide analogs, termed metabolic chemical reporters (MCRs), th...
Development of a silicon phthalocyanine analogue for near-infrared photoimmunotherapy and its application to HTLV-1-infected leukemic cells [0.03%]
一种近红外光免疫治疗的硅酞菁类似物及其在HTLV-1感染白血病细胞中的应用
Yoshikazu Fuse,Eita Sasaki,Masaharu Tamaki et al.
Yoshikazu Fuse et al.
Near-infrared photoimmunotherapy (NIR-PIT) employing an antibody labeled with a silicon phthalocyanine dye, IR700, was approved as a minimally invasive treatment for unresectable recurrent head and neck cancer in Japan in 2020. However, fur...