Self-cleaving ribozymes: substrate specificity and synthetic biology applications [0.03%]
自我剪切核酶：底物特异性和合成生物学应用
Huan Peng,Brandon Latifi,Sabine Müller et al.
Huan Peng et al.
Various self-cleaving ribozymes appearing in nature catalyze the sequence-specific intramolecular cleavage of RNA and can be engineered to catalyze cleavage of appropriate substrates in an intermolecular fashion, thus acting as true catalys...
Lin-Lin Zhou,Hongjiao Xu,Yue Huang et al.
Lin-Lin Zhou et al.
N 6-Methyladenosine (m6A) is the most prevalent internal modification on mRNA and represents a new layer of gene expression in eukaryotes. The field of m6A-encoded epitranscriptomics was rejuvenated with the discovery of fat mass and obesit...
Faster, better, and cheaper: harnessing microfluidics and mass spectrometry for biotechnology [0.03%]
更快，更好，更便宜：利用微流控和质谱技术为生物技术保驾护行
Noel S Ha,Markus de Raad,La Zhen Han et al.
Noel S Ha et al.
High-throughput screening technologies are widely used for elucidating biological activities. These typically require trade-offs in assay specificity and sensitivity to achieve higher throughput. Microfluidic approaches enable rapid manipul...
19F NMR viewed through two different lenses: ligand-observed and protein-observed 19F NMR applications for fragment-based drug discovery [0.03%]
通过两种不同的透镜看待19F核磁共振：基于片段的药物发现中观察到的配体和蛋白质的19F核磁共振应用
Caroline R Buchholz,William C K Pomerantz
Caroline R Buchholz
19F NMR has emerged as a powerful tool in drug discovery, particularly in fragment-based screens. The favorable magnetic resonance properties of the fluorine-19 nucleus, the general absence of fluorine in biological settings, and its ready ...
Correction: Rational design of a stapled JAZ9 peptide inhibiting protein-protein interaction of a plant transcription factor [0.03%]
关于植物转录因子蛋白质-蛋白质相互作用抑制的环肽的设计的更正通知
Kaho Suzuki,Yousuke Takaoka,Minoru Ueda
Kaho Suzuki
[This corrects the article DOI: 10.1039/D0CB00204F.]. This journal is © The Royal Society of Chemistry.
Published Erratum
RSC chemical biology. 2021 Jan 26;2(2):669. DOI:10.1039/d1cb90002a 2021
Correction: Antibacterial activity of a dual peptide targeting the Escherichia coli sliding clamp and the ribosome [0.03%]
关于抗细菌活性的一项更正：双肽作用于大肠杆菌滑动夹钳和核糖体
Christophe André,Florian Veillard,Philippe Wolff et al.
Christophe André et al.
[This corrects the article DOI: 10.1039/D0CB00060D.]. This journal is © The Royal Society of Chemistry.
Published Erratum
RSC chemical biology. 2021 Jul 6;2(4):1296. DOI:10.1039/d1cb90020j 2021
Wenchao Lu,Milka Kostic,Tinghu Zhang et al.
Wenchao Lu et al.
[This corrects the article DOI: 10.1039/D0CB00222D.]. This journal is © The Royal Society of Chemistry.
Published Erratum
RSC chemical biology. 2021 Feb 22;2(2):670-671. DOI:10.1039/d1cb90008k 2021
Correction: Antibody recruiting molecules (ARMs): synthetic immunotherapeutics to fight cancer [0.03%]
Correction: 抗体募集分子（ARM）：对抗癌症的合成免疫治疗药物
Silvia Achilli,Nathalie Berthet,Olivier Renaudet
Silvia Achilli
[This corrects the article DOI: 10.1039/D1CB00007A.]. This journal is © The Royal Society of Chemistry.
Published Erratum
RSC chemical biology. 2021 Jul 22;2(4):1297-1300. DOI:10.1039/d1cb90021h 2021
Orthogonal coiled coils enable rapid covalent labelling of two distinct membrane proteins with peptide nucleic acid barcodes [0.03%]
正交盘绕螺旋使具有肽核酸条形码的两种不同的膜蛋白迅速发生共价标记
Georgina C Gavins,Katharina Gröger,Marc Reimann et al.
Georgina C Gavins et al.
Templated chemistry offers the prospect of addressing specificity challenges occurring in bioconjugation reactions. Here, we show two peptide-templated amide-bond forming reactions that enable the concurrent labelling of two different membr...
Phosphinate esters as novel warheads for activity-based probes targeting serine proteases [0.03%]
用于靶向丝氨酸蛋白酶的活性基于探针的新型弹头磷inic酸酯
Jan Pascal Kahler,Steven H L Verhelst
Jan Pascal Kahler
Activity-based protein profiling enables the specific detection of the active fraction of an enzyme and is of particular use for the profiling of proteases. The technique relies on a mechanism-based reaction between small molecule activity-...