George S M Hanson,Faidra Batsaki,Teagan L Myerscough et al.
George S M Hanson et al.
Proline cis/trans isomerism plays an important role in protein folding and mediating protein-protein interactions in short linear interacting motifs within intrinsically disordered protein regions. The slow exchange rate between cis and tra...
Sources of mismeasurement of RNA knockdown by DNAzymes and XNAzymes [0.03%]
DNAzyme和XNAzyme介导的RNA敲低测量误差来源分析
Maria J Donde,Alicia Montulet,Alexander I Taylor
Maria J Donde
RNA-cleaving oligonucleotide catalysts composed of DNA and/or nucleic acid analogues (DNAzymes, modified DNAzymes and XNAzymes) are promising agents for specific knockdown of disease-associated RNAs. However, we and others have identified d...
Deciphering protein long-chain S-acylation using mass spectrometry proteomics strategies [0.03%]
利用质谱蛋白质组学策略解读蛋白质长链S-酰化密码
Anneroos E Nederstigt,Samiksha Sardana,Marc P Baggelaar
Anneroos E Nederstigt
Protein long-chain S-acylation, the reversible attachment of fatty acids such as palmitate to cysteine residues via thioester bonds, is a widespread post-translational modification that plays a crucial role in regulating protein localizatio...
Fitness landscapes and thermodynamic approaches to development of nucleic acids enzymes: from classical methods to AI integration [0.03%]
适应度景观和核酸酶发育的热力学方法：从经典方法到AI集成
Shuntaro Takahashi,Michiaki Hamada,Hisae Tateishi-Karimata et al.
Shuntaro Takahashi et al.
Nucleic acids (NA), namely DNA and RNA, dynamically fold and unfold to perform their functions in cells. Functional NAs include NA enzymes, such as ribozymes and DNAzymes. Their folding and target binding are governed by interactions betwee...
Photoresponsive molecular tweezers modulate Taspase 1 activity [0.03%]
光响应分子夹可通过调节Taspase 1活性来控制蛋白质加工
Antonio L Figueroa Bietti,Alisa-Maite A Kauth,Katrin Hommel et al.
Antonio L Figueroa Bietti et al.
Light serves as an exceptional stimulus for the precise spatiotemporal regulation of protein activity and protein-protein interactions. Here, we introduce a light-responsive supramolecular ligand system designed to modulate Taspase 1, a pro...
Plasma membrane labelling efficiency, internalization and partitioning of functionalized fluorescent lipids as a function of lipid structure [0.03%]
荧光素标记的脂肪结构与细胞膜标记效率、内吞作用及分区的关系研究
Erdinc Sezgin
Erdinc Sezgin
Labeling the plasma membrane for advanced imaging remains a significant challenge. For time-lapse live cell imaging, probe internalization and photobleaching are major limitations affecting most membrane-specific dyes. In fixed or permeabil...
The bacterial stress response polymerase DinB tolerates sugar modifications and preferentially incorporates arabinosyl nucleotides [0.03%]
细菌胁迫反应聚合酶DinB能够耐受糖基化修饰并优先掺入阿拉伯糖核苷酸
Christina M Hurley,Jeffrey M Kubiak,Michael B Cory et al.
Christina M Hurley et al.
The bacterial DNA damage (SOS) response promotes DNA repair, DNA damage tolerance, and survival in the setting of genotoxic stress, including stress induced by antibiotics. In E. coli, translesion DNA synthesis can be fulfilled by Y-family ...
Discovery of an exquisitely selective WDR5 chemical probe accelerated by a high-quality DEL-ML Hit [0.03%]
高质量的DNA编码文库命中物导向的WDR5极异性小分子化学探针的发现
Lasse Hoffmann,Christopher Lenz,Frederic Farges et al.
Lasse Hoffmann et al.
Herein we present the rapid development of LH168, a potent and highly selective chemical probe for WDR5, streamlined by utilizing a DEL-ML (DNA encoded library-machine learning) hit as the chemical starting point. LH168 was comprehensively ...
Contributions of β-lactamase substrate specificity and outer membrane permeability to the antibiotic sheltering of β-lactam-susceptible bacteria [0.03%]
β-内酰胺酶底物特异性和外膜通透性对β-内酰胺敏感菌抗生素避难的贡献
Montserrat Mora-Ochomogo,Mitchell A Jeffs,Josephine L Liu et al.
Montserrat Mora-Ochomogo et al.
The use of β-lactam antibiotics is threatened by antibiotic resistant bacteria that produce β-lactamases. These enzymes not only protect the bacteria that produce them but also shelter other bacteria in the same environment that would oth...
Peptide nucleic acids in parallel orientation form invasion complexes with double-stranded DNA [0.03%]
肽核酸平行结合形成入侵双链DNA复合物
Masanari Shibata,Hiroshi Sugimoto,Masaki Hibino et al.
Masanari Shibata et al.
Peptide nucleic acid (PNA) is a unique class of synthetic nucleic acids with a pseudo-peptide backbone, known for its high nucleic acid recognition capability and its ability to directly recognize double-stranded DNA (dsDNA) via the formati...