Semi-enzymatic synthesis and application of 13C-isotopically labelled inositol-(1,4,5)-trisphosphate [0.03%]
半酶法合成及应用同位素标记的肌醇(1，4，5)三磷酸
Atharva Patharkar,Meike Amma,Jaime Isern et al.
Atharva Patharkar et al.
Inositol-(1,4,5)-trisphosphate (Ins(1,4,5)P3) is a crucial secondary messenger that controls calcium (Ca2+) levels inside cells, yet many questions regarding Ins(1,4,5)P3 metabolism are challenging to address with current methods. Here, a s...
P450 cyptide synthase MpoB catalyzes the cross-linking of the YPW motif on the precursor peptide [0.03%]
P450细胞色素合成酶MpoB催化前体肽上YPW基序交联反应的进行
Abujunaid Habib Khan,Jabal Rahmat Haedar,Vic Kiselov et al.
Abujunaid Habib Khan et al.
Cytochrome P450 enzymes in ribosomally synthesized and post-translationally modified peptides (RiPPs) catalyze C-C, C-N, or C-O cross-linking reactions in the biosynthesis of biaryl cyclophane natural products. Here, we manually identified ...
Regioselective rapid ene-type reaction (RRER) enables bioconjugation of histone serotonylation [0.03%]
区域选择性快速ene型反应(RRER)可实现组蛋白血清素化的生物连接
Jinghua Wu,Huapeng Li,Adam R Lovato et al.
Jinghua Wu et al.
Triazolinedione (TAD) derivatives have been commonly utilized as protection and labeling reagents for indole and phenol moieties via a reversible ene-type reaction. Previous studies showed that the TAD probes could selectively modify tyrosi...
Signals from the sea: the structural peculiarity of lipid A and weak immunostimulatory lipopolysaccharide from Rheinheimera japonica [0.03%]
来自海洋的信号：Rheinheimera japonica脂A的独特结构及其弱免疫刺激作用的脂多糖分子机制研究
Stefania De Chiara,Francesca Olmeo,Emanuela Andretta et al.
Stefania De Chiara et al.
Lipopolysaccharides (LPSs) isolated from marine bacteria represent a valuable resource for biomedical innovation. Here, we report the first structural elucidation of the lipid A moiety and a preliminary immunological assessment of the full ...
Chemoenzymatic synthesis of sialylated and fucosylated mucin analogs reveals glycan-dependent effects on protein conformation and degradation [0.03%]
化学酶法合成唾液酸化和岩藻糖基化的黏蛋白类似物揭示了糖链对蛋白质构象和降解的依赖关系
Amanda M Wood,Casia L Wardzala,Jessica R Kramer
Amanda M Wood
Mucin proteins are essential for life but are challenging to study due to their complex glycosylation patterns. Synthetic mimics have become vital tools for understanding and modulating the roles of mucins in human health and disease. These...
Random peptide mixtures of tryptophan and lysine suppress the aggregation of a cancer-related mutant of the Axin protein [0.03%]
含有色氨酸和赖氨酸的随机多肽混合物可抑制癌症相关变异型Axin蛋白的聚集
Tommaso Garfagnini,Zvi Hayouka,Assaf Friedler
Tommaso Garfagnini
Aggregation of dysfunctional proteins can lead to a variety of diseases including cancer. We have previously developed chaperone-derived peptides that inhibit aggregation of the cancer-related L106R mutant of Axin RGS. Here we show that sig...
Structure-guided engineering of a polyphosphate kinase 2 class III from an Erysipelotrichaceae bacterium to produce base-modified purine nucleotides [0.03%]
结构导向工程化 erysipelotrichaceae 细菌的多聚磷酸酶 2 类 III 以产生碱基修饰嘌呤核苷酸
Rachel M Mitton-Fry,René Rasche,Ann-Marie Lawrence-Dörner et al.
Rachel M Mitton-Fry et al.
Nucleobase-modified nucleoside-5'-triphosphates (NTPs) are important building blocks for the enzymatic synthesis of non-coding RNAs and mRNAs with improved properties. Chemical phosphorylation of base-modified nucleotides to NTPs remains ch...
The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples [0.03%]
一种快速定量粗样本结合动力学的SpyBLI无细胞流水线技术
Olga Predeina,Misha Atkinson,Oliver Wissett et al.
Olga Predeina et al.
Accurate measurements of binding kinetics, encompassing equilibrium dissociation constant (K D), association rate (k on), and dissociation rate (k off), are critical for the development and optimisation of high-affinity binding proteins. Ho...
Exploring the immunoproteasome's substrate preferences for improved hydrolysis and selectivity [0.03%]
探究免疫蛋白酶体底物偏好以改善其水解和选择性性能
Christine S Muli,Cody A Loy,Darci J Trader
Christine S Muli
The proteasome is an integral macromolecular machine responsible for regulated protein degradation, and its barrel-like core particle (CP) hydrolyzes protein substrates into peptide fragments. A proteasome isoform that is expressed under co...
A dual-functional substrate for quantitation of substrate levels and GCase activity in living cells [0.03%]
一种用于定量活细胞底物水平和GCase活性的双功能底物
Ben Tiet,Sha Zhu,Xi Chen et al.
Ben Tiet et al.
Loss of function mutations in the gene GBA1, which encodes the lysosomal glycoside hydrolase β-glucocerebrosidase (GCase) cause Gaucher's disease (GD). Moreover, one mutant allele of GBA1 is the most common genetic risk factor for the deve...