Understanding insulin-like peptide 5 (INSL5) and relaxin family peptide receptor 4 (RXFP4): structure, signalling, and function [0.03%]
理解胰岛素样肽5（INSL5）和relaxin家族肽受体4（RXFP4）：结构、信号转导及功能
Hongkang Wu,Huahao Wang,Isabelle Riches et al.
Hongkang Wu et al.
Insulin-like peptide 5 (INSL5) is a member of the relaxin/insulin peptide family, predominantly produced by enteroendocrine L cells in the distal gut. Although initially characterised based on its structural similarity to insulin and relaxi...
A two-step bioconjugation of S. aureus lipoteichoic acid (LTA) affords fluorescent probes that illuminate the interaction between Gram-positive glycolipids and mammalian cell membranes [0.03%]
金黄色葡萄球菌脂磷壁酸的两步生物偶联反应得到能发光的探针并用于揭示革兰氏阳性细菌脂类和哺乳动物细胞膜之间的相互作用
Jake Henry,Joe Nabarro,Harriet Chidwick et al.
Jake Henry et al.
Lipoteichoic acid (LTA), a major constituent of Gram-positive bacteria cell wall, is an amphiphilic glycolipid and well-established stimulator of immune cells through activation of Toll-Like Receptor 2 (TLR2) complexes. LTA binding to TLR2 ...
The position of hydrophobic residues impacts cellular uptake and intracellular localization of cell penetrating peptides [0.03%]
疏水残基的位置影响细胞穿透肽的细胞摄取和胞内定位
Adeline Schmitt,Helma Wennemers
Adeline Schmitt
Cell-penetrating peptides (CPPs) are widely used to deliver cargo into mammalian cells, yet efficient cellular uptake and endosomal escape remain key challenges. In this study, we evaluated how hydrophobic (4S)-4-cyclohexylproline (ChPro) r...
Scarless one-tube genome assembly via computationally optimized uracil-DNA glycosylase reactions [0.03%]
通过计算优化的尿嘧啶-dna-glycosylase反应实现无痕单管基因组组装
Rachel M Carson,Patrick M Needham,Pedro J Fernandez Mendoza et al.
Rachel M Carson et al.
Synthetic biology enables the creation of systems such as bacteriophage (phage)-based biosensors, leveraging the innate specificity and efficiency of phages to rapidly identify pathogens. However, the current genome assembly and editing met...
Real-time monitoring of glycocarrier formation unravels cryptic details in glycosyl transfer [0.03%]
实时监测糖载体的形成揭露了糖基转移中的隐秘细节
Ryan D Packer,Angelo Gallo,Alexander D Cameron et al.
Ryan D Packer et al.
Glycosyl transfer constitutes a key step in the functionalisation of bioactive molecules, including proteins and natural products. In vitro studies to dissect glycosyltransferase catalysis are often challenging due to poor enzyme solubility...
Cooperative membrane association as a mechanistic origin of synergistic antimicrobial peptide activity [0.03%]
协同性膜定位作用是抗菌肽活性的协同性的机制起源
Angela Medvedeva,Anatoly B Kolomeisky
Angela Medvedeva
Antimicrobial peptides (AMPs) are central components of the innate immunity system that can be found in almost all living organisms. They are promising alternatives to conventional antibiotics due to their broad-spectrum activity and reduce...
Synthesis and characterization of a novel phosphatidylinositol 5-phosphate (PI(5)P) photoaffinity probe [0.03%]
一种新型磷脂酰肌醇5-磷酸（PI(5)P）光亲和探针的合成与表征
Glen Brodie,Ahmed Sayed,Sarah Kreuz et al.
Glen Brodie et al.
Phosphatidylinositol 5-phosphate (PI(5)P) plays a crucial role in cellular signaling, cell proliferation, the DNA damage repair response, and gene transcription. However, the underlying mechanism of PI(5)P function in these cellular pathway...
Tipping the balance: synthesis and evaluation of centrinone-based degraders of polo-like kinase 4 [0.03%]
tipping the balance：基于centrinone的极光激酶4降解剂的合成与评估
Andrej Kovacevic,Aleksandar Salim,Crisálida Borges et al.
Andrej Kovacevic et al.
Polo-like kinase 4 (PLK4) is a serine/threonine-protein kinase that plays a pivotal role in centriole biogenesis and, as such, represents a master regulator of centriole duplication. Due to its importance in cancer development and progressi...
Let the peptides shine: SOX (Sulfonamido-OXine)-labelled peptides for direct kinase and phosphatase monitoring [0.03%]
让肽类发光：SOX（Sulfonamido-OXine）标记的肽直接监测激酶和磷酸酶
Lydia E Papagora,Stephen A Cochrane
Lydia E Papagora
Protein kinases and their cognate phosphatases are central regulators of cellular signalling, acting through the opposing processes of phosphorylation and dephosphorylation. Defects in kinase/phosphatase signalling are implicated in many di...
Aneesah Jabar,Mikayla Pinto,Terence E Hébert
Aneesah Jabar
5-HT2A receptors have been shown to play critical roles in regulating neuronal signalling networks and brain plasticity. They have become important targets for treatment of resistant forms of mental health disorders such as depression, anxi...