New insights into the structure and dynamics of the epigenetic modifications on DNA [0.03%]
DNA表观遗传修饰结构和动力学的新认识
Dineshbabu Takkella,Javier Cerezo,Lara Martinez-Fernandez et al.
Dineshbabu Takkella et al.
DNA methylation is a key epigenetic modification involved in genomic imprinting, X-chromosome inactivation, and repression of repetitive element transcription and transposition. Despite its biological significance, the impact of epigenetic ...
Chemically inducible antisense oligonucleotides for cell-specific gene silencing [0.03%]
化学诱导的反义寡核苷酸用于细胞特异性基因沉默
Zhen Xun,Yang Hai,Li-Juan Tang et al.
Zhen Xun et al.
Cell-specific control of the function of antisense oligonucleotides (ASOs) is highly desirable for precise gene therapy while minimizing adverse effects in normal cells. Herein, we report a novel class of chemically inducible ASOs (iASOs) t...
Selective labeling and visualization of viral and bacterial neuraminidases using ortho-quinone methide-based probes [0.03%]
选择性标记和可视化病毒和细菌神经氨酸酶的邻甲基醌衍生物探针方法
Erianna I Alvarado-Melendez,Simon T Ruessink,Karin Strijbis et al.
Erianna I Alvarado-Melendez et al.
Neuraminidases (NAs) are critical virulence factors in pathogens. In viruses such as influenza A, neuraminidase facilitates the release of virions, thereby enabling infection propagation. In pathogenic bacteria, NA activity has been linked ...
Alkyne-tagged ribitol-5-phosphate derivatives for metabolic labelling of alpha-dystroglycan [0.03%]
用于α- dystroglycan代谢标记的富马酸核糖醇-5-磷酸衍生物
Lloyd D Murphy,Saeed Akkad,Angelo Lopez et al.
Lloyd D Murphy et al.
The glycoprotein α-dystroglycan is essential in establishing cell-matrix interactions and is implicated in the pathology of muscular dystrophies. Novel tools are needed to study its rare and intriguing O-mannosyl glycans. This report descr...
Glyco-functionalization of ECM mimics, influence in morphology and cell behaviour [0.03%]
仿细胞外基质的糖基化功能修饰及其对细胞形态和行为的影响研究
Maddalena Bracchi,Francesco Nicotra,Laura Russo
Maddalena Bracchi
Current tissue engineering strategies primarily focus on replicating mechanical properties of the extracellular matrix (ECM); however, several studies have underscored the critical role of the ECM biochemical cues in developing functional t...
Thiazole peptidomimetics as chemical modulators of KRAS gene expression via G-quadruplex stabilization [0.03%]
噻唑肽模拟物通过G-四链体稳定化作用作为KRAS基因表达的化学调节剂
Debasmita Biswas,Ananta Gorai,Sandip Maiti et al.
Debasmita Biswas et al.
KRAS is one of the most frequently mutated oncogenes in human cancers and remains a challenging target for therapeutic intervention, often labeled "undruggable." We herein synthesized triazole-containing peptidomimetics TTh1 and TTh2, to ex...
Biocatalytic synthesis of phenyl benzoate esters using the amide ligase ClxA [0.03%]
ClxA酰胺酶催化苯甲酸苯基酯的生物合成
Alexander Ascham,Qingyun Tang,Ian J S Fairlamb et al.
Alexander Ascham et al.
The synthesis of ester bonds using lipases is one of the most frequently performed reactions in biocatalysis, yet examples of the enzymatic synthesis of phenyl benzoate esters are comparatively rare. In this report we show that the ligase C...
Experimental identification of preQ1-binding RNAs in the pathogenic bacterium Listeria monocytogenes [0.03%]
listeria monocytogenes致病菌中preq1结合rna的实验识别
Malou Hanisch,Laurin Flemmich,Christoph Mitteregger et al.
Malou Hanisch et al.
Riboswitches are widespread regulatory RNA modules in bacteria, with many different classes already identified and even more yet to be discovered. Traditionally, the identification of riboswitches has relied on bioinformatic analyses and ge...
Discovery of microbial glycoside hydrolases via enrichment and metaproteomics [0.03%]
通过富集和元蛋白质组学发现微生物糖苷水解酶
Jitske M van Ede,Suzanne van der Steen,Geert M van der Kraan et al.
Jitske M van Ede et al.
The immense microbial diversity on Earth represents a vast genomic resource, yet discovering novel enzymes from complex environments remains challenging. Here, we combine a microbial enrichment with metagenomics and metaproteomics to facili...
A Lyt at the end of the tunnel? Unraveling the complex interactions of the N-acetylglucosaminidase LytG in cell wall metabolism [0.03%]
隧道尽头的Lyt？解开N-乙酰葡萄糖胺酶LytG在细胞壁代谢中的复杂相互作用
Jazmeen Hernandez,Jett Duval,Taryn Rauff et al.
Jazmeen Hernandez et al.
The growth and division of the Gram-positive cell requires the coordinated action of enzymes involved in the synthesis and degradation of the heteropolymer peptidoglycan. Herein, we present the use of the diamide masarimycin, an inhibitor o...