Contributions of β-lactamase substrate specificity and outer membrane permeability to the antibiotic sheltering of β-lactam-susceptible bacteria [0.03%]
β-内酰胺酶底物特异性和外膜通透性对β-内酰胺敏感菌抗生素避难的贡献
Montserrat Mora-Ochomogo,Mitchell A Jeffs,Josephine L Liu et al.
Montserrat Mora-Ochomogo et al.
The use of β-lactam antibiotics is threatened by antibiotic resistant bacteria that produce β-lactamases. These enzymes not only protect the bacteria that produce them but also shelter other bacteria in the same environment that would oth...
Peptide nucleic acids in parallel orientation form invasion complexes with double-stranded DNA [0.03%]
肽核酸平行结合形成入侵双链DNA复合物
Masanari Shibata,Hiroshi Sugimoto,Masaki Hibino et al.
Masanari Shibata et al.
Peptide nucleic acid (PNA) is a unique class of synthetic nucleic acids with a pseudo-peptide backbone, known for its high nucleic acid recognition capability and its ability to directly recognize double-stranded DNA (dsDNA) via the formati...
Reductively activated CPP-PROTAC nanocomplexes enhance target degradation via efficient cellular uptake [0.03%]
可还原激活的CPP- PROTAC纳米复合物通过高效细胞摄取增强目标降解
Maho Miyamoto,Kosuke Saito,Hidetomo Yokoo et al.
Maho Miyamoto et al.
We developed a nanoparticle based on a cell-penetrating peptide-PROTAC conjugate with a disulfide linker, MZ1-R9, and dextran sulfate, enhancing cellular uptake and BRD4 degradation. This delivery platform significantly improves PROTAC bioa...
Differential melting voltage by tandem-trapped ion mobility spectrometry: glycan structure influences glycoprotein stability [0.03%]
串联俘获离子淌度谱的差示熔化电压：糖结构影响糖蛋白稳定性
Mengqi Chai,Christian Bleiholder,Fanny C Liu
Mengqi Chai
Profiling the full spectrum of protein glycoforms is critical to understanding their functional roles. We developed the differential melting voltage approach using tandem-ion mobility/tandem-mass spectrometry and applied it to study Ribonuc...
Detection and characterisation of ligand-induced conformational changes in acetylcholine binding proteins using biosensors and X-ray crystallography [0.03%]
基于生物传感器和X射线晶体学的乙酰胆碱结合蛋白配体诱导构象变化的检测与表征
Edward A FitzGerald,Daniela Cederfelt,Daria Kovryzhenko et al.
Edward A FitzGerald et al.
Analysis of ligand-induced structural changes in proteins is challenging due to the lack of experimental methods suited for detection and characterisation of both ligand binding and induced structural changes. We have explored biosensors wi...
Characterization of nuclease stability and poly(A)-binding protein binding activity of chemically modified poly(A) tail for in vivo applications [0.03%]
化学修饰的聚腺苷酸(Poly(A))尾巴的核酸酶稳定性和多聚腺苷酸结合蛋白(poly(A)-bindingprotein，PABP)结合活性分析及其体内应用研究
Atsushi Hashimoto,Yuma Kunitomo,Ittoku Kikuchi et al.
Atsushi Hashimoto et al.
The poly(A) tail plays a crucial role in mRNA stability and translation efficiency. Chemical modification of the poly(A) tail is a promising approach for stabilizing mRNA against deadenylation. In this study, we investigated the effect of p...
Applications of click and click-to-release chemistry in biomaterials to advance skin regeneration [0.03%]
点击化学和“点击释放”化学在皮肤再生生物材料中的应用研究进展
Merel Gansevoort,Matthijs van de Waarsenburg,Thomas J Boltje et al.
Merel Gansevoort et al.
Achieving skin regeneration following destruction of the epidermis and dermis (e.g. full-thickness wounds) has remained an unachieved goal. The wound healing response is complex and consists of multiple overlapping phases which are tightly ...
Investigating the N-terminal linker histone H1 subtypes as substrates for JmjC lysine demethylases [0.03%]
探究N端连接组蛋白H1亚型作为JmjC赖氨酸脱甲基酶底物的功能
Vildan A Türkmen,Anthony Tumber,Eidarus Salah et al.
Vildan A Türkmen et al.
Members of the Jumonji C (JmjC) subfamily of non-heme Fe(ii) and 2-oxoglutarate (2OG) dependent N ε-lysine demethylases have established roles in catalysing demethylation of N ε-methylated lysine residues in core histones; their roles in ...
Copper-catalysed azide-alkyne cycloaddition on live M13 bacteriophage for expanding the molecular diversity of phage-displayed peptide libraries [0.03%]
用于扩大噬菌体肽展示文库分子多样性的活M13噬菌体上的铜催化叠氮炔基环加成反应
Olabode Dawodu,Cody A White,Caitlin Specht et al.
Olabode Dawodu et al.
Phage display is a powerful platform for ligand evolution, but conventional phage display libraries are confined to the twenty canonical amino acids, greatly limiting the chemical space that these libraries can be used to explore. Here we p...
Sheel C Dodani,Ariel Furst
Sheel C Dodani
As both chemical and biological engineering approaches continue to expand, the landscape of biomolecular technologies is rapidly evolving, affording new opportunities from basic science to real-world applications. This themed collection bri...
Editorial
RSC chemical biology. 2025 Aug 19. DOI:10.1039/d5cb90031j 2025