Structural basis of SIRT2 pre-catalysis NAD+ binding dynamics and mechanism [0.03%]
SIRT2 催化前NAD+结合动力学和机制的结构基础
Nan Zhang,Kah Chee Pow,Lanfang Chen et al.
Nan Zhang et al.
Sirtuins are an evolutionarily conserved family of NAD+-dependent deacylases whose catalytic mechanism remains under active investigation. While previous studies have captured sirtuin reaction intermediates using thioacetyl-lysine analogs, ...
Biosynthesis of sydonol reveals a new bisabolene cyclase and an unusual P450 aromatase [0.03%]
_sydonol的生物合成揭示了一种新的倍半蒈烯环化酶和一种不寻常的P450芳构化酶
Peiyu Lu,Ling Liu
Peiyu Lu
In this work, the biosynthetic gene cluster and assembly line of sydonol were discovered and identified. As a result, and importantly, two unusual chemical conversions catalyzed by a new bisabolene cyclase and an unusual P450 aromatase were...
George S M Hanson,Faidra Batsaki,Teagan L Myerscough et al.
George S M Hanson et al.
Proline cis/trans isomerism plays an important role in protein folding and mediating protein-protein interactions in short linear interacting motifs within intrinsically disordered protein regions. The slow exchange rate between cis and tra...
Sources of mismeasurement of RNA knockdown by DNAzymes and XNAzymes [0.03%]
DNAzyme和XNAzyme介导的RNA敲低测量误差来源分析
Maria J Donde,Alicia Montulet,Alexander I Taylor
Maria J Donde
RNA-cleaving oligonucleotide catalysts composed of DNA and/or nucleic acid analogues (DNAzymes, modified DNAzymes and XNAzymes) are promising agents for specific knockdown of disease-associated RNAs. However, we and others have identified d...
Deciphering protein long-chain S-acylation using mass spectrometry proteomics strategies [0.03%]
利用质谱蛋白质组学策略解读蛋白质长链S-酰化密码
Anneroos E Nederstigt,Samiksha Sardana,Marc P Baggelaar
Anneroos E Nederstigt
Protein long-chain S-acylation, the reversible attachment of fatty acids such as palmitate to cysteine residues via thioester bonds, is a widespread post-translational modification that plays a crucial role in regulating protein localizatio...
Fitness landscapes and thermodynamic approaches to development of nucleic acids enzymes: from classical methods to AI integration [0.03%]
适应度景观和核酸酶发育的热力学方法：从经典方法到AI集成
Shuntaro Takahashi,Michiaki Hamada,Hisae Tateishi-Karimata et al.
Shuntaro Takahashi et al.
Nucleic acids (NA), namely DNA and RNA, dynamically fold and unfold to perform their functions in cells. Functional NAs include NA enzymes, such as ribozymes and DNAzymes. Their folding and target binding are governed by interactions betwee...
Photoresponsive molecular tweezers modulate Taspase 1 activity [0.03%]
光响应分子夹可通过调节Taspase 1活性来控制蛋白质加工
Antonio L Figueroa Bietti,Alisa-Maite A Kauth,Katrin Hommel et al.
Antonio L Figueroa Bietti et al.
Light serves as an exceptional stimulus for the precise spatiotemporal regulation of protein activity and protein-protein interactions. Here, we introduce a light-responsive supramolecular ligand system designed to modulate Taspase 1, a pro...
Plasma membrane labelling efficiency, internalization and partitioning of functionalized fluorescent lipids as a function of lipid structure [0.03%]
荧光素标记的脂肪结构与细胞膜标记效率、内吞作用及分区的关系研究
Erdinc Sezgin
Erdinc Sezgin
Labeling the plasma membrane for advanced imaging remains a significant challenge. For time-lapse live cell imaging, probe internalization and photobleaching are major limitations affecting most membrane-specific dyes. In fixed or permeabil...
The bacterial stress response polymerase DinB tolerates sugar modifications and preferentially incorporates arabinosyl nucleotides [0.03%]
细菌胁迫反应聚合酶DinB能够耐受糖基化修饰并优先掺入阿拉伯糖核苷酸
Christina M Hurley,Jeffrey M Kubiak,Michael B Cory et al.
Christina M Hurley et al.
The bacterial DNA damage (SOS) response promotes DNA repair, DNA damage tolerance, and survival in the setting of genotoxic stress, including stress induced by antibiotics. In E. coli, translesion DNA synthesis can be fulfilled by Y-family ...
Discovery of an exquisitely selective WDR5 chemical probe accelerated by a high-quality DEL-ML Hit [0.03%]
高质量的DNA编码文库命中物导向的WDR5极异性小分子化学探针的发现
Lasse Hoffmann,Christopher Lenz,Frederic Farges et al.
Lasse Hoffmann et al.
Herein we present the rapid development of LH168, a potent and highly selective chemical probe for WDR5, streamlined by utilizing a DEL-ML (DNA encoded library-machine learning) hit as the chemical starting point. LH168 was comprehensively ...