Bioinformatic and biochemical analysis uncovers novel activity in the 2-ER subfamily of OYEs [0.03%]
生物信息学和生化分析揭示了OYE的2-ER亚家族的新活性
Tamra C Blue-Lahom,Stacey K Jones,Katherine M Davis
Tamra C Blue-Lahom
Members of the old yellow enzyme (OYE) family utilize a flavin mononucleotide cofactor to catalyze the asymmetric reduction of activated alkenes. The 2-enoate reductase (2-ER) subfamily are of particular industrial relevance as they can red...
Raman signatures of type A and B influenza viruses: molecular origin of the " catch and kill" inactivation mechanism mediated by micrometric silicon nitride powder [0.03%]
甲型和乙型流感病毒的拉曼标记：“捕杀”灭活机制的分子起源及微米级硅氮化物粉末介导方式
Giuseppe Pezzotti,Yoshiki Yasukochi,Eriko Ohgitani et al.
Giuseppe Pezzotti et al.
A multiomic study of the structural characteristics of type A and B influenza viruses by means of highly spectrally resolved Raman spectroscopy is presented. Three virus strains, A H1N1, A H3N2, and B98, were selected because of their known...
Novel photocrosslinking chemical probes utilized for high-resolution spatial transcriptomics [0.03%]
新型光交联化学探针用于高分辨率空间转录组学研究
Leslie Spitalny,Natalie Falco,Whitney England et al.
Leslie Spitalny et al.
The architecture of cells and the tissue they form within multicellular organisms are highly complex and dynamic. Cells optimize their function within tissue microenvironments by expressing specific subsets of RNAs. Advances in cell tagging...
Siderophore-based targeted antibody recruitment for promoting immune responses towards Gram-negative pathogens [0.03%]
基于铁载体的靶向抗体募集促进针对革兰氏阴性病原体的免疫反应
Seungwoo Kim,Ho-Sung Park,Do Young Kim et al.
Seungwoo Kim et al.
Antibody-recruiting molecules (ARMs) have emerged as a promising strategy for enhancing immune responses against pathogens and cancer cells. In this study, we developed a novel class of antibacterial ARMs utilizing siderophores, small iron-...
Raman active diyne-girder conformationally constrained p53 stapled peptides bind to MDM2 for visualisation without fluorophores [0.03%]
用于无荧光标记的MDM2可视化具有构象限制的p53 Stapled肽结合到MDM2中的二炔衍生物骨架肽中RAMAN活性站点
Danielle C Morgan,Laura McDougall,Astrid Knuhtsen et al.
Danielle C Morgan et al.
Peptide stapling is an effective strategy to stabilise α-helical peptides, enhancing their bioactive conformation and improving physiochemical properties. In this study, we apply our novel diyne-girder stapling approach to the MDM2/MDMX α...
Using environment-sensitive tetramethylated thiophene-BODIPY fluorophores in DNA probes for studying effector-induced conformational changes of protein-DNA complexes [0.03%]
利用环境敏感的四甲基噻吩-BODIPY荧光染料研究效应子诱导的蛋白-DNA复合物构象变化
Markéta Šoltysová,Pedro Güixens-Gallardo,Irena Sieglová et al.
Markéta Šoltysová et al.
The LutR protein represses the transcription of genes encoding enzymes for the utilization of l-lactate in Bacillus subtilis through binding to a specific DNA region. In this study, we employed oligonucleotide probes modified by viscosity-s...
Xuefei Jing,Joel P Mackay,Toby Passioura
Xuefei Jing
Targeted protein degraders, in the form of proteolysis targeting chimaeras (PROTACs) and molecular glues, leverage the ubiquitin-proteasome system to catalytically degrade specific target proteins of interest. Because such molecules can be ...
Uncovering the substrate of olefin synthase loading domains in cyanobacteria Picosynechococcus sp. strain PCC 7002 [0.03%]
解密集胞氰细菌Picosynechococcus sp.菌株PCC 7002中烯烃合酶装载结构域的底物
Audrey E Yñigez-Gutierrez,Erin Conley,Michael G Thomas et al.
Audrey E Yñigez-Gutierrez et al.
Cyanobacteria are widespread, photosynthetic, gram-negative bacteria that generate numerous bioactive secondary metabolites via complex biosynthetic enzymatic machinery. The model cyanobacterium Picosynechococcus sp. strain PCC 7002, hereaf...
A one-step route for the conversion of Cd waste into CdS quantum dots by Acidithiobacillus sp. via unique biosynthesis pathways [0.03%]
硫化细菌通过独特生物合成途径将Cd废物转化为CdS量子点的一锅法路径
Xiao-Ju Li,Tian-Qi Wang,Lu Qi et al.
Xiao-Ju Li et al.
Microorganisms serve as biological factories for the synthesis of nanomaterials such as CdS quantum dots. Based on the uniqueness of Acidithiobacillus sp., a one-step route was explored to directly convert cadmium waste into CdS QDs using t...
The molecular features of non-peptidic nucleophilic substrates and acceptor proteins determine the efficiency of sortagging [0.03%]
非肽类亲核底物和受体蛋白的分子特征决定了索塔改性的效率
Tetiana Bondarchuk,Elena Zhuravel,Oleh Shyshlyk et al.
Tetiana Bondarchuk et al.
Sortase A-mediated ligation (SML) or "sortagging" has become a popular technology to selectively introduce structurally diverse protein modifications. Despite the great progress in the optimization of the reaction conditions and design of m...