Protein ligation for the assembly and study of nonribosomal peptide synthetase megaenzymes [0.03%]
蛋白质连接用于非核糖体肽合成酶超大酶的组装与研究
Angelos Pistofidis,T Martin Schmeing
Angelos Pistofidis
Nonribosomal peptide synthetases (NRPSs) are biosynthetic enzymes found in bacteria and fungi, that synthesize a plethora of pharmaceutically relevant compounds. NRPSs consist of repeating sets of functional domains called modules, and each...
Reem Mousa,Dana Shkolnik,Yam Alalouf et al.
Reem Mousa et al.
Chemical protein synthesis has emerged as a powerful approach for producing ubiquitin (Ub) and ubiquitin-like modifiers (Ubls) in both their free and conjugated forms, particularly when recombinant or enzymatic strategies are challenging. B...
Bioinformatic, structural, and biochemical analysis leads to the discovery of novel isonitrilases and decodes their substrate selectivity [0.03%]
生物信息学、结构和生化分析发现新型异氰酸酯酶并解码其底物选择性
Tyler Hostetler,Tzu-Yu Chen,Wei-Chen Chang
Tyler Hostetler
Bacterial species, such as Mycobacterium tuberculosis, utilize isonitrile-containing peptides (INPs) for trace metal trafficking, e.g., copper or zinc. Despite their importance, very few INP structures have been characterized to date. Repor...
Development of selective nanomolar cyclic peptide ligands as GBA1 enzyme stabilisers [0.03%]
GBA1酶稳定剂的纳摩尔级环肽配体的开发
Rebecca E Katzy,Renier H P van Neer,Maria J Ferraz et al.
Rebecca E Katzy et al.
The stabilisation of recombinant glycosidases by exogenous ligands, known as pharmacological chaperones or enzyme stabilisers, has recently garnered great clinical interest. This strategy can prevent enzyme degradation in the blood, reducin...
Jun Miyazaki,Ryotaro Wagatsuma,Koji Okamoto
Jun Miyazaki
In solid tumours, cancer cells modify their metabolic processes to endure environments with nutrient and oxygen scarcity due to inadequate blood flow. A thorough understanding of this adaptive mechanism, which requires reliable microscopic ...
Development of a near-infrared fluorescent probe for the selective detection of severe hypoxia [0.03%]
用于检测重度缺氧的近红外荧光探针的开发
Takafumi Kasai,Kyohhei Fujita,Toru Komatsu et al.
Takafumi Kasai et al.
Severely hypoxic environments with oxygen concentrations around 1% are often found in serious diseases such as ischemia and cancer. However, existing near-infrared (NIR) fluorescent probes that can visualize hypoxia are also activated in mi...
Site-specific in vivo protein SUMOylation via translational incorporation of a proximity-reactive pyrrolysine analogue [0.03%]
利用近端反应性吡咯赖氨酸类似物的翻译掺入实现位点特异性体内蛋白质SUMO化修饰
Yuk Hei Chan,Marianne M Lee,Michael K Chan
Yuk Hei Chan
Here, we present a novel strategy that integrates genetic-code expansion and proximity-induced crosslinking to achieve site-specific in vivo SUMOylation. This approach involves incorporating the unnatural amino acid 2-chloroacetyl-Nε-lysin...
Biochemical characterization and discovery of inhibitors for Pf Sir2A: new tricks for an old enzyme [0.03%]
PfSir2A的生化表征及其抑制剂的发现：旧酶的新功能
Dickson Donu,Emily Boyle,Alyson Curry et al.
Dickson Donu et al.
The Sir2 enzyme from Plasmodium falciparum (PfSir2A) is essential for the antigenic variation of this parasite, and its inhibition is expected to have therapeutic effects for malaria. Selective PfSir2A inhibitors are not available yet, part...
RPRD1B's direct interaction with phosphorylated RNA polymerase II regulates polyadenylation of cell cycle genes and drives cancer progression [0.03%]
RPRD1B与磷酸化RNA聚合酶II的直接相互作用调节细胞周期基因多聚腺苷酸化并驱动癌症进展
Rosamaria Y Moreno,Svetlana B Panina,Y Jessie Zhang
Rosamaria Y Moreno
RNA polymerase II (Pol II) regulates eukaryotic gene expression through dynamic phosphorylation of its C-terminal domain (CTD). Phosphorylation at Ser2 and Thr4 on the CTD is crucial for RNA 3' end processing and facilitating the recruitmen...
Navigating the dichotomy of reactive oxygen, nitrogen, and sulfur species: detection strategies and therapeutic interventions [0.03%]
活性氧、氮和硫化物的双刃剑性质：检测策略与治疗干预措施
Prayasee Baruah,Dikshaa Padhi,Hariharan Moorthy et al.
Prayasee Baruah et al.
Reactive oxygen, nitrogen and sulfur species (RONSS) collectively encompasses a variety of energetically dynamic entities that emerge as inherent characteristics of aerobic life. This broad category includes reactive oxygen species (ROS), r...