Illuminating microtubule functions with small molecules: past, present and future of fluorescent tubulin-binding probes [0.03%]
揭秘微管功能的荧光结合小分子探针：过去、现在和将来
R París-Ogáyar,D Lucena-Agell,K-H Altmann et al.
R París-Ogáyar et al.
Developing tools for the real-time visualization of tubulin, microtubules, and their dynamics is essential for studying key cellular processes such as cell division, migration, and differentiation. In this context, fluorescent small-molecul...
Bifunctional glycolipids targeting TLR4·MD-2 and short pentraxins [0.03%]
兼具TLR4·MD-2和短五聚蛋白受体靶点的双功能甘油糖脂分子
Daniele Zucchetta,Lena Nuschy,Simon Gumpelmair et al.
Daniele Zucchetta et al.
Innate immune detection of pathogen- and danger-associated molecular patterns (PAMPs/DAMPs) centres on pattern-recognition receptors, with the TLR4/MD-2 complex being uniquely sensitive to trace levels of lipopolysaccharide (LPS) as well as...
Fluorescent cationic fluorinated oxazoliniums for cysteine bioconjugation via an SNAr reaction [0.03%]
通过SNAr反应用于半胱氨酸生物连接的荧光阳离子含氟噁唑𬭩化合物
Karen Ka-Yan Kung,Yosephine Tania Limanto,Ajcharapan Tantipanjaporn et al.
Karen Ka-Yan Kung et al.
Using cationic fluorinated oxazolinium compounds, fluorescent cysteine-selective SNAr bioconjugation proceeds under mild conditions, resulting in fluorescent-labelled peptides and proteins with moderate to excellent conversions of up to 99%...
Integration of palladium-catalyzed C-N coupling into self-encoded libraries for accelerated hit discovery [0.03%]
钯催化C-N偶联反应在自编码组合库中的应用及加速药物活性化合物发现的方法研究
Edith van der Nol,Zhenshuo Luo,Qing Qing Gao et al.
Edith van der Nol et al.
Affinity screenings with encoded libraries are transformative tools for rapid hit discovery from vast compound collections. Yet the adaptation of established chemical reactions to DNA-encoded libraries (DELs) remains challenging due to DNA-...
Comparative analysis of alkyne- and desthiobiotinylated photoaffinity probes for chemotranscriptomic profiling [0.03%]
用于化学转录组学谱分析的经炔基和脱硫生物素衍生光亲和探针的比较研究
Daphne A L van den Homberg,Georgia Poulladofonou,Aurelia Dekens et al.
Daphne A L van den Homberg et al.
Understanding small molecule-RNA interactions is a crucial part in drug development and fundamental biology. Chemotranscriptomic profiling is emerging as a powerful platform to interrogate interactions of small molecules with entire transcr...
Kota Nomura,Keiji Numata
Kota Nomura
Post-translational modifications (PTMs) endow silk proteins with chemical diversity that governs their higher-order assembly, hydration, and covalent connectivity. This review highlights the principal PTMs that define silk protein function,...
Chemical modulation of the unfolded protein response reveals an antiviral role for the PERK pathway in human coronavirus 229E infection [0.03%]
化学调节未折叠蛋白反应发现PERK通路在人类冠状病毒229E感染中具有抗病毒作用
Isabella E Pellizzari-Delano,Trinity H Tooley,Debanjana Mondal et al.
Isabella E Pellizzari-Delano et al.
Broad spectrum antivirals are critical to respond rapidly to the threat posed by newly emerging RNA viruses. One potential candidate is the natural compound thapsigargin (Tg). Tg potently induces endoplasmic reticulum (ER) stress and activa...
Development of a transcription factor-based biosensor strain for reporting α-terpineol production via the alcohol-dependent hemiterpene pathway in Escherichia coli [0.03%]
开发一种基于转录因子的生物传感器菌株用于通过依赖于醇类的半萜途径在大肠杆菌中报告α-萜品醇的生产
Catherine A Odhiambo,Isaac A Ali,Gavin J Williams
Catherine A Odhiambo
Terpenes constitute a vast and industrially important class of natural products. Yet, microbial production of many high-value terpenoids remains limited by the difficulty of rationally engineering their biosynthetic pathways and the lack of...
Structure and characterisation of CMP-Kdn synthetase from the haptophyte microalgae Prymnesium parvum [0.03%]
一种触丝藻 Prymnesium parvum 的CMP-Kdo 合酶的结构与表征
Claire Morley,Alexandra J Munro-Clark,Ben A Wagstaff et al.
Claire Morley et al.
Sialic acids - 9-carbon ulosonic acids - are implicated in many cell-cell and host-pathogen interactions due to their prevalent location at the non-reducing end of glycoconjugates. Sialic acids have recently been observed in microalgae, inc...
Discovery of natural products that modulate signaling in patient-derived cells [0.03%]
发现可调节患者源细胞信号传导的天然物质
Joseph A Balsamo,Hannah L Thirman,Kathryn E Penton et al.
Joseph A Balsamo et al.
Natural products derived from biosynthetically apt microorganisms represent an important source for chemotherapeutic agents founded first via non-specific, cytotoxicity-guided isolation, then investigated for translational potential. Analyz...