Max T B Clabbers,Michael W Martynowycz,Johan Hattne et al.
Max T B Clabbers et al.
Microcrystal electron diffraction (MicroED) is a powerful technique utilizing electron cryo-microscopy (cryo-EM) for protein structure determination of crystalline samples too small for X-ray crystallography. Electrons interact with the ele...
Selective observation of semi-rigid non-core residues in dynamically complex mutant huntingtin protein fibrils [0.03%]
动态复杂突变亨廷顿蛋白纤维中半刚性非核心残基的选择性观察
Irina Matlahov,Jennifer C Boatz,Patrick C A van der Wel
Irina Matlahov
Many amyloid-forming proteins, which are normally intrinsically disordered, undergo a disorder-to-order transition to form fibrils with a rigid β-sheet core flanked by disordered domains. Solid-state NMR (ssNMR) and cryogenic electron micr...
Subtomogram averaging for biophysical analysis and supramolecular context [0.03%]
用于生物物理分析和超分子背景的子体素平均法
Lauren Ann Metskas,Rosalie Wilfong,Grant J Jensen
Lauren Ann Metskas
Recent advances in hardware, software and computing power have led to increasingly ambitious applications of cryo-electron tomography and subtomogram averaging. It is now possible to reveal both structures and biophysical relationships like...
Biological solid-state NMR: Integrative across different scientific disciplines [0.03%]
生物固態核磁共振：不同科學領域的整合
Marc Baldus
Marc Baldus
For almost five decades, solid-state NMR (ssNMR) has been used to study complex biomolecular systems. This article gives a view on how ssNMR methods and applications have evolved during this time period in a broader structural biology conte...
In-cell DNP NMR reveals multiple targeting effect of antimicrobial peptide [0.03%]
细胞内DNP-NMR揭示了抗菌肽的多重靶向效应
Frances Separovic,Vinzenz Hofferek,Anthony P Duff et al.
Frances Separovic et al.
Dynamic nuclear polarization NMR spectroscopy was used to investigate the effect of the antimicrobial peptide (AMP) maculatin 1.1 on E. coli cells. The enhanced 15N NMR signals from nucleic acids, proteins and lipids identified a number of ...
Nucleic acid-protein interfaces studied by MAS solid-state NMR spectroscopy [0.03%]
利用固态NMR研究核酸-蛋白质相互作用界面
Philipp Innig Aguion,Alexander Marchanka,Teresa Carlomagno
Philipp Innig Aguion
Solid-state NMR (ssNMR) has become a well-established technique to study large and insoluble protein assemblies. However, its application to nucleic acid-protein complexes has remained scarce, mainly due to the challenges presented by overl...
POKY software tools encapsulating assignment strategies for solution and solid-state protein NMR data [0.03%]
用于溶液和固态蛋白质NMR数据的POKY软件工具封装赋值策略
Ira Manthey,Marco Tonelli,Lawrence Clos Ii et al.
Ira Manthey et al.
NMR spectroscopy provides structural and functional information about biomolecules and their complexes. The complexity of these systems can make the NMR data difficult to interpret, particularly for newer users of NMR technology, who may ha...
The role of C-terminal helix in the conformational transition of an arginine binding protein [0.03%]
C-末端螺旋在精氨廀结合蛋白构象变化中的作用
Vinothini Santhakumar,Nahren Manuel Mascarenhas
Vinothini Santhakumar
The thermotoga maritima arginine binding protein (TmArgBP) is a periplasmic binding protein that has a short helix at the C-terminal end (CTH), which is swapped between the two chains. We apply a coarse-grained structure-based model (SBM) a...
SAA fibrils involved in AA amyloidosis are similar in bulk and by single particle reconstitution: A MAS solid-state NMR study [0.03%]
利用固体状态核磁共振研究的AA淀粉样变性中涉及的SAA纤维在总体和单粒子重构上相似
Arpita Sundaria,Falk Liberta,Dilan Savran et al.
Arpita Sundaria et al.
AA amyloidosis is one of the most prevalent forms of systemic amyloidosis and affects both humans and other vertebrates. In this study, we compare MAS solid-state NMR data with a recent cryo-EM study of fibrils involving full-length murine ...
Solid-state NMR analysis of unlabeled fungal cell walls from Aspergillus and Candida species [0.03%]
无标记曲霉和念珠菌细胞壁的固态NMR分析研究
Liyanage D Fernando,Malitha C Dickwella Widanage,S Chandra Shekar et al.
Liyanage D Fernando et al.
Fungal infections cause high mortality in immunocompromised individuals, which has emerged as a significant threat to human health. The efforts devoted to the development of antifungal agents targeting the cell wall polysaccharides have bee...