Heterotopic mineral deposits in intact rat Achilles tendons are characterized by a unique fiber-like structure [0.03%]
完整的鼠跟腱异位矿化沉积物具有独特的纤维状结构
Maria Pierantoni,Malin Hammerman,Isabella Silva Barreto et al.
Maria Pierantoni et al.
Heterotopic mineralization entails pathological mineral formation inside soft tissues. In human tendons mineralization is often associated with tendinopathies, tendon weakness and pain. In Achilles tendons, mineralization is considered to o...
BioAFMviewer software for simulation atomic force microscopy of molecular structures and conformational dynamics [0.03%]
用于模拟分子结构和构象动力学的原子力显微镜的BioAFMviewer软件
Romain Amyot,Noriyuki Kodera,Holger Flechsig
Romain Amyot
Atomic force microscopy (AFM) and high-speed scanning have significantly advanced real time observation of biomolecular dynamics, with applications ranging from single molecules to the cellular level. To facilitate the interpretation of res...
Measuring the effects of ice thickness on resolution in single particle cryo-EM [0.03%]
测定冰厚对单颗粒冷冻电镜分辨率的影响
Kasahun Neselu,Bing Wang,William J Rice et al.
Kasahun Neselu et al.
Ice thickness is a critical parameter in single particle cryo-EM - too thin ice can break during imaging or exclude the sample of interest, while ice that is too thick contributes to more inelastic scattering that precludes obtaining high r...
Subtomogram analysis: The sum of a tomogram's particles reveals molecular structure in situ [0.03%]
子全素分析：一个全素图中颗粒的总和揭示了分子结构所在的位置
Friedrich Förster
Friedrich Förster
Cryo-electron tomography is uniquely suited to provide insights into the molecular architecture of cells and tissue in the native state. While frozen hydrated specimens tolerate sufficient electron doses to distinguish different types of pa...
The structure of a Bacteroides thetaiotaomicron carbohydrate-binding module provides new insight into the recognition of complex pectic polysaccharides by the human microbiome [0.03%]
通过人类微生物群了解复杂果胶多糖识别的新见解及肠道细菌碳水化合物结合模块的结构
Filipa Trovão,Viviana G Correia,Frederico M Lourenço et al.
Filipa Trovão et al.
The Bacteroides thetaiotaomicron has developed a consortium of enzymes capable of overcoming steric constraints and degrading, in a sequential manner, the complex rhamnogalacturonan II (RG-II) polysaccharide. BT0996 protein acts in the init...
The Ewald sphere/focus gradient does not limit the resolution of cryoEM reconstructions [0.03%]
低温电子显微镜重建的分辨率不受Ewald球/焦点梯度的限制
J Bernard Heymann
J Bernard Heymann
In our quest to solve biomolecular structures to higher resolutions in cryoEM, care must be taken to deal with all aspects of image formation in the electron microscope. One of these is the Ewald sphere/focus gradient that derives from the ...
Makoto Tokoro Schreiber,Alan Maigné,Marco Beleggia et al.
Makoto Tokoro Schreiber et al.
It is well known that insulating samples can accumulate electric charges from exposure to an electron beam. How the accumulation of charge affects imaging parameters and sample stability in transmission electron microscopy is poorly underst...
Assignment of aromatic side-chain spins and characterization of their distance restraints at fast MAS [0.03%]
芳香族测链自旋的指定及在快速MAS下对它们距离限制的特征化
Sahil Ahlawat,Subbarao Mohana Venkata Mopidevi,Pravin P Taware et al.
Sahil Ahlawat et al.
The assignment of aromatic side-chain spins has always been more challenging than assigning backbone and aliphatic spins. Selective labeling combined with mutagenesis has been the approach for assigning aromatic spins. This manuscript repor...
Characterizing the resolution and throughput of the Apollo direct electron detector [0.03%]
阿波罗直接电子检测器的分辨率和吞吐量的特征分析
Ruizhi Peng,Xiaofeng Fu,Joshua H Mendez et al.
Ruizhi Peng et al.
Advances in electron detection have been essential to the success of high-resolution cryo-EM structure determination. A new generation of direct electron detector called the Apollo, has been developed by Direct Electron. The Apollo uses a n...
Aromatic ring flips in differently packed ubiquitin protein crystals from MAS NMR and MD [0.03%]
来自固态NMR和MD的结构不同韵 ubiquitin 蛋白晶体中的芳香基团翻转
Diego F Gauto,Olga O Lebedenko,Lea Marie Becker et al.
Diego F Gauto et al.
Probing the dynamics of aromatic side chains provides important insights into the behavior of a protein because flips of aromatic rings in a protein's hydrophobic core report on breathing motion involving a large part of the protein. Inhere...