Structure of SARS-CoV-2 MTase nsp14 with the inhibitor STM957 reveals inhibition mechanism that is shared with a poxviral MTase VP39 [0.03%]
SARS-CoV-2 MTase nsp14与抑制剂STM957的复合物结构揭示了一种与痘病毒MTase VP39共有的抑制机制
Eva Zilecka,Martin Klima,Milan Stefek et al.
Eva Zilecka et al.
Nsp14 is an RNA methyltransferase (MTase) encoded by all coronaviruses. In fact, many viral families, including DNA viruses, encode MTases that catalyze the methylation of the RNA precap structure, resulting in fully capped viral RNA. This ...
Do selectivity filter carbonyls in K+ channels flip away from the pore? Two-dimensional infrared spectroscopy study [0.03%]
钾通道的选择性过滤器羰基是面向孔道内部还是背离孔道？二维红外光谱研究
Nikhil Maroli,Matthew J Ryan,Martin T Zanni et al.
Nikhil Maroli et al.
Molecular dynamics simulations revealed that the carbonyls of the Val residue in the conserved selectivity filter sequence TVGTG of potassium ion channels can flip away from the pore to form hydrogen bonds with the network of water molecule...
Yasmeen N Ruma,Guanhong Bu,Johan Hattne et al.
Yasmeen N Ruma et al.
Clostripain secreted from Clostridium histolyticum is the founding member of the C11 family of Clan CD cysteine peptidases, which is an important group of peptidases secreted by numerous bacteria. Clostripain is an arginine-specific endopep...
Accurate size-based protein localization from cryo-ET tomograms [0.03%]
基于尺寸的蛋白质定位的准确性来自cryo-ET断层扫描图像
Weisheng Jin,Ye Zhou,Alberto Bartesaghi
Weisheng Jin
Cryo-electron tomography (cryo-ET) combined with sub-tomogram averaging (STA) allows the determination of protein structures imaged within the native context of the cell at near-atomic resolution. Particle picking is an essential step in th...
Nucleic acid-binding KH domain proteins influence a spectrum of biological pathways including as part of membrane-localized complexes [0.03%]
核苷酸结合的KH结构域蛋白会影响一系列生物学途径，包括作为膜定位复合物的一部分参与其中
Md Kamrul Hasan,L Jeannine Brady
Md Kamrul Hasan
K-Homology domain (KH domain) proteins bind single-stranded nucleic acids, influence protein-protein interactions of proteins that harbor them, and are found in all kingdoms of life. In concert with other functional protein domains KH domai...
Solution structure, dynamics and tetrahedral assembly of Anti-TRAP, a homo-trimeric triskelion-shaped regulator of tryptophan biosynthesis in Bacillus subtilis [0.03%]
枯草芽孢杆菌中反式作用调节蛋白Anti-TRAP的溶液结构、动态和四面体组装机制研究（英文）
Craig A McElroy,Elihu C Ihms,Deepak Kumar Yadav et al.
Craig A McElroy et al.
Cellular production of tryptophan is metabolically expensive and tightly regulated. The small Bacillus subtilis zinc binding Anti-TRAP protein (AT), which is the product of the yczA/rtpA gene, is upregulated in response to accumulating leve...
Magic-angle spinning NMR structure of Opa60 in lipid bilayers [0.03%]
magic-angle spinning NMR 脂质双层中的 Opa60 结构
Marcel C Forster,Kumar Tekwani Movellan,Eszter E Najbauer et al.
Marcel C Forster et al.
Here we report the structure of Opa60 in lipid bilayers using proton-detected magic-angle spinning nuclear magnetic resonance (MAS NMR). Preparations including near-native oligosaccharide lipids reveal a consistent picture of a stable trans...
Cody Gillman,Guanhong Bu,Emma Danelius et al.
Cody Gillman et al.
Microcrystal electron diffraction (MicroED) has emerged as a powerful technique for unraveling molecular structures from microcrystals too small for X-ray diffraction. However, a significant hurdle arises with plate-like crystals that consi...
Optimizing NMR fragment-based drug screening for membrane protein targets [0.03%]
基于片段的药物筛选技术在膜蛋白靶点中的优化应用
Geoffrey C Li,Manuel A Castro,Thilini Ukwaththage et al.
Geoffrey C Li et al.
NMR spectroscopy has played a pivotal role in fragment-based drug discovery by coupling detection of weak ligand-target binding with structural mapping of the binding site. Fragment-based screening by NMR has been successfully applied to ma...
Coordination of bilayer properties by an inward-rectifier K+ channel is a cooperative process driven by protein-lipid interaction [0.03%]
由蛋白质-脂质相互作用驱动的内向整流钾通道协调双层特性的协同过程
Evan J van Aalst,Maryam Yekefallah,Roy A M van Beekveld et al.
Evan J van Aalst et al.
Physical properties of biological membranes directly or indirectly govern biological processes. Yet, the interplay between membrane and integral membrane proteins is difficult to assess due to reciprocal effects between membrane proteins, i...