Production and cryo-electron microscopy structure of an internally tagged SARS-CoV-2 spike ecto-domain construct [0.03%]
内部标记的SARS-CoV-2刺突胞外域结构的生产及冷冻电镜结构分析
Suruchi Singh,Yi Liu,Meghan Burke et al.
Suruchi Singh et al.
The SARS-CoV-2 spike protein is synthesized in the endoplasmic reticulum of host cells, from where it undergoes export to the Golgi and the plasma membrane or retrieval from the Golgi to the endoplasmic reticulum. Elucidating the fundamenta...
Non-uniform Fourier transform based image classification in single-particle Cryo-EM [0.03%]
基于非均匀傅里叶变换的单粒子冷冻电镜图像分类
ZiJian Bai,Jian Huang
ZiJian Bai
In the single-particle Cryo-EM projection image classification, it is a common practice to apply the Fourier transform to the images and extract rotation-invariant features in the frequency domain. However, this process involves interpolati...
Protein identification using Cryo-EM and artificial intelligence guides improved sample purification [0.03%]
使用冷冻电镜和人工智能识别蛋白质可指导改进样品纯化过程
Kenneth D Carr,Dane Evan D Zambrano,Connor Weidle et al.
Kenneth D Carr et al.
Protein purification is essential in protein biochemistry, structural biology, and protein design, enabling the determination of protein structures, the study of biological mechanisms, and the characterization of both natural and de novo de...
SidF, a dual substrate N5-acetyl-N5-hydroxy-L-ornithine transacetylase involved in Aspergillus fumigatus siderophore biosynthesis [0.03%]
参与烟曲霉铁载体生物合成的双底物N5-乙酰-N5-羟基-L-鸟氨酸转乙酰酶SidF
Thanalai Poonsiri,Jan Stransky,Nicola Demitri et al.
Thanalai Poonsiri et al.
Siderophore-mediated iron acquisition is essential for the virulence of Aspergillus fumigatus, a fungus causing life-threatening aspergillosis. Drugs targeting the siderophore biosynthetic pathway could help improve disease management. The ...
Natalie Reznikov
Natalie Reznikov
Corrigendum to "Minimizing ice contamination during specimen preparation for cryo-soft X-ray tomography and cryo-electron tomography" [J. Struct. Biol.: X 10(2024) 100113] [0.03%]
关于“减少冷冻软X射线层析成像和冷冻电子层析成像样品制备过程中冰污染”的勘误[J. Struct. Biol.: X 10(2024) 100113]
Chia-Chun Hsieh,Zi-Jing Lin,Lee-Jene Lai
Chia-Chun Hsieh
[This corrects the article DOI: 10.1016/j.yjsbx.2024.100113.]. © 2024 The Author(s).
Published Erratum
Journal of structural biology: X. 2024 Oct 30:10:100115. DOI:10.1016/j.yjsbx.2024.100115 2024
Structural analysis of the stable form of fibroblast growth factor 2 - FGF2-STAB [0.03%]
FGF2-STAB的结构分析
Gabin de La Bourdonnaye,Martin Marek,Tereza Ghazalova et al.
Gabin de La Bourdonnaye et al.
Fibroblast growth factor 2 (FGF2) is a signaling protein that plays a significant role in tissue development and repair. FGF2 binds to fibroblast growth factor receptors (FGFRs) alongside its co-factor heparin, which protects FGF2 from degr...
Assessment of submicron bone tissue composition in plastic-embedded samples using optical photothermal infrared (O-PTIR) spectral imaging and machine learning [0.03%]
基于光学光热红外(O-PTIR)光谱成像和机器学习评估塑料包埋样品中亚微米骨组织成分
Isha Dev,Sofia Mehmood,Nancy Pleshko et al.
Isha Dev et al.
Understanding the composition of bone tissue at the submicron level is crucial to elucidate factors contributing to bone disease and fragility. Here, we introduce a novel approach utilizing optical photothermal infrared (O-PTIR) spectroscop...
Minimizing ice contamination during specimen preparation for cryo-soft X-ray tomography and cryo-electron tomography [0.03%]
用于cryo-软X射线断层扫描和cryo-电子断层扫描的样品制备以减少冰冻污染
Chia-Chun Hsieh,Zi-Jing Lin,Lee-Jene Lai
Chia-Chun Hsieh
Cryo-soft X-ray tomography (cryo-SXT) is a newly developed technique for imaging 3D whole cells in nearly native states. Cryo-SXT users require the preparation of numerous cryo-sample grids to use the allocated beamtime to study cellular ph...
Conformational variability in the D2 loop of Plasmodium Apical Membrane antigen 1 [0.03%]
恶性疟原虫(ap)-膜蛋白1(d2环)的构象变动性研究
Frederick A Saul,Brigitte Vulliez-Le Normand,Alexander Boes et al.
Frederick A Saul et al.
Apical Membrane Antigen 1 (AMA1) plays a vital role in the invasion of the host erythrocyte by the malaria parasite, Plasmodium. It is thus an important target for vaccine and anti-malaria therapeutic strategies that block the invasion proc...