Optimization of negative stage bias potential for faster imaging in large-scale electron microscopy [0.03%]
大尺度电子显微镜成像快速化的负偏压优化方法研究
Ryan Lane,Yoram Vos,Anouk H G Wolters et al.
Ryan Lane et al.
Large-scale electron microscopy (EM) allows analysis of both tissues and macromolecules in a semi-automated manner, but acquisition rate forms a bottleneck. We reasoned that a negative bias potential may be used to enhance signal collection...
Structural flexibility of the tetanus neurotoxin revealed by crystallographic and solution scattering analyses [0.03%]
基于晶体学和溶液散射分析的破伤风神经毒素结构灵活性的研究
Chun-Ming Zhang,Yoshihiro Imoto,Takaaki Hikima et al.
Chun-Ming Zhang et al.
Although the tetanus neurotoxin (TeNT) delivers its protease domain (LC) across the synaptic vesicle lumen into the cytosol via its receptor binding domain (HC) and translocation domain (HN), the molecular mechanism coordinating this membra...
Structural basis of enzyme activity regulation by the propeptide of l-lysine α-oxidase precursor from Trichoderma viride [0.03%]
绿霉L-赖氨酸α氧化酶前体信号肽对其酶活调节的结构基础研究
Masaki Kitagawa,Nanako Ito,Yuya Matsumoto et al.
Masaki Kitagawa et al.
Harmuful proteins are usually synthesized as inactive precursors and are activated by proteolytic processing. l-Amino acid oxidase (LAAO) is a flavoenzyme that catalyzes the oxidative deamination of l-amino acid to produce a 2-oxo acid with...
Contrasting DNA-binding behaviour by ISL1 and LHX3 underpins differential gene targeting in neuronal cell specification [0.03%]
ISL1和LHX3不同的DNA结合行为决定了神经元细胞特化过程中的基因靶向差异
Ngaio C Smith,Lorna E Wilkinson-White,Ann H Y Kwan et al.
Ngaio C Smith et al.
The roles of ISL1 and LHX3 in the development of spinal motor neurons have been well established. Whereas LHX3 triggers differentiation into interneurons, the additional expression of ISL1 in developing neuronal cells is sufficient to redir...
Structure and intermolecular interactions in spheroidal high-density lipoprotein subpopulations [0.03%]
球形高密度脂蛋白亚群的结构与分子间相互作用
Chris J Malajczuk,Neha S Gandhi,Ricardo L Mancera
Chris J Malajczuk
Human serum high-density lipoproteins (HDLs) are a population of small, dense protein-lipid aggregates that are crucial for intravascular lipid trafficking and are protective against cardiovascular disease. The spheroidal HDL subfraction ca...
A guided approach for subtomogram averaging of challenging macromolecular assemblies [0.03%]
挑战性的大型分子装配的亚颗粒平均的指导方法
Benjamin Basanta,Saikat Chowdhury,Gabriel C Lander et al.
Benjamin Basanta et al.
Joseph Caesar,Cyril F Reboul,Chiara Machello et al.
Joseph Caesar et al.
We here introduce the third major release of the SIMPLE (Single-particle IMage Processing Linux Engine) open-source software package for analysis of cryogenic transmission electron microscopy (cryo-EM) movies of single-particles (Single-Par...
FMN riboswitch aptamer symmetry facilitates conformational switching through mutually exclusive coaxial stacking configurations [0.03%]
对映的fmn核糖开关适配体对称性通过互斥的同轴堆积构型促进结构转换
Haley M Wilt,Ping Yu,Kemin Tan et al.
Haley M Wilt et al.
Knowledge of both apo and holo states of riboswitches aid in elucidating the various mechanisms of ligand-induced conformational "switching" that underpin their gene-regulating capabilities. Previous structural studies on the flavin mononuc...
Structures and dynamics of the novel S1/S2 protease cleavage site loop of the SARS-CoV-2 spike glycoprotein [0.03%]
新型冠状病毒刺突糖蛋白新型S1/S2蛋白酶切割位点环的结构和动力学研究
Thomas Lemmin,David Kalbermatter,Daniel Harder et al.
Thomas Lemmin et al.
At the end of 2019, a new highly virulent coronavirus known under the name SARS-CoV-2 emerged as a human pathogen. One key feature of SARS-CoV-2 is the presence of an enigmatic insertion in the spike glycoprotein gene representing a novel m...
C O S Sorzano,F de Isidro-Gómez,E Fernández-Giménez et al.
C O S Sorzano et al.
Electron tomography is a technique to obtain three-dimensional structural information of samples. However, the technique is limited by shifts occurring during acquisition that need to be corrected before the reconstruction process. In 2009,...