Prolongation of mitosis is associated with enhanced endogenous DNA damage in fission yeast [0.03%]
细胞内源性DNA损伤与裂殖酵母有丝分裂延长有关
Ishutesh Jain,Phong T Tran
Ishutesh Jain
Mitosis is usually shorter than other phases of the cell cycle and maintains a consistent duration despite variations in cell size and spindle size. This suggests the existence of a compensatory mechanism that ensures a short duration, poss...
A simulation test of the prediction that density-dependent dispersal promotes female-biased sex allocation in viscous populations [0.03%]
密度依赖扩散促进黏性种群中雌偏性性别分配的预测仿真测试
Chedhawat Chokechaipaisarn,Andy Gardner
Chedhawat Chokechaipaisarn
A classic result of sex-allocation theory is that the sex ratio is predicted to be invariant with respect to the rate of dispersal. However, a recent mathematical analysis has suggested that if individuals are able to adjust their probabili...
A missense mutation in the C. elegans src-2 tyrosine-protein kinase reduces brood size and enhances embryonic morphogenesis defects in src-1(RNAi) conditions [0.03%]
秀丽隐杆线虫src-2酪氨酸蛋白质激酶错义突变体的遗传学分析
Xiaofei Bai,Rebecca Green,Tao Cai et al.
Xiaofei Bai et al.
Goldenhar Syndrome is a rare congenital disorder characterized by hemifacial microsomia. Although select mutations have been mapped for this disorder, the genetic etiologies in the majority of cases remain unknown. A recent clinical report ...
Characterizing short germline-specific promoters with a range of expression levels in C. elegans [0.03%]
具有不同表达水平的短特化胚系启动子的特性研究——以秀丽隐杆线虫为例
Mohammed D Aljohani,Sonia El Mouridi,Christian Frøkjær-Jensen
Mohammed D Aljohani
A core tenet of synthetic biology is that well-characterized regulatory elements are essential for engineering biological systems. Here, we characterize the specificity and expression levels of 18 short (254 to 880 bp) candidate germline pr...
AraC-induced neuron-like differentiation of human NTERA2/D1 cells and quantification of endogenous pre-mir-106b and 19b levels [0.03%]
AraC诱导的人类NTERA2/D1细胞神经元样分化及内源性pre-mir-106b和19b水平的测定
Yuka Kaneko,Tomoko Takahashi
Yuka Kaneko
MicroRNAs (miRNAs) are approximately 22 nucleotide-long non-coding RNAs that are encoded in the genome. miRNAs form base pairs with target mRNAs in the RNA-induced silencing complex and repress their expression through a mechanism called RN...
Sensitive detection of lipopolysaccharides by monitoring of interleukin-10 secretion from human PBMCs [0.03%]
人PBMCs分泌IL-10监测灵敏测定脂多糖
Daniel Ivanusic,Joachim Denner
Daniel Ivanusic
Lipopolysaccharide (LPS) contaminations may falsify immunological experiments and are crucial for pharmaceutical products because they cause life-threatening immune reactions. Here, we present interleukin-10 (IL-10) as a reliable marker to ...
Replication stress by MMS stimulates DNA synthesis in post-replicative G2-phase in S. pombe [0.03%]
MMS诱发的复制压力可激活模式生物裂殖酵母G2期的DNA复制机制
Seong Min Kim,Susan L Forsburg
Seong Min Kim
DNA replication is generally limited to S-phase but replication stress can drive cells to undergo DNA synthesis outside of S-phase. Mitotic DNA synthesis pathway is known to be activated to deal with replication stress-induced chromosomal i...
Quantifying the Over-retraction (Ore) phenotype in C. elegans male tail tip morphogenesis [0.03%]
量化C. elegans雄性尾尖形态发生的过度回缩(Ore)表型
Julia Burnett,E Jane Albert Hubbard
Julia Burnett
Morphometrics, the quantitative analysis of biological structures, reduces subjectivity and increases reproducibility in characterizing morphological phenotypes. In C. elegans males, the rounded adult tail tip emerges from a stage-specific ...
Co-culture with normal and senescent AC16 cardiomyocytes modulates fibrotic response in primary human ventricular fibroblasts [0.03%]
正常和衰老的AC16心肌细胞共培养通过调节转化生长因子-β信号影响人 ventricular成纤维细胞纤维化反应
Veronica Hidalgo,Nikhitha Kastury,Cheyanne W Durham et al.
Veronica Hidalgo et al.
Transwell co-culture with human AC16 cardiomyocyte-like cells modifies the response of primary human ventricular fibroblasts to TGF-β stimulation. Fibrotic response markers including collagen I (COL1A1) and ɑ-smooth muscle actin (ACTA2) a...
Senescence in human AC16 cardiac cells is associated with thymidine kinase induction and histone loss [0.03%]
人AC16心肌细胞衰老与胸苷激酶诱导和组蛋白丢失有关
Nikhitha Kastury,Veronica Hidalgo,Boomathi Pandi et al.
Nikhitha Kastury et al.
AC16 cells are a transformed human cardiac cell line commonly used to study cardiomyocyte biology. We show that reduced proliferation and senescence markers can be robustly induced in AC16 cells cultured in low serum condition and treated w...