Cultured cells activate IRE1 during attachment and flattening after routine passaging [0.03%]
培养的细胞在附着和传代后的扁平化过程中激活IRE1
Paige Dillon,Lincoln Hollingshead,Julie Hollien
Paige Dillon
During endoplasmic reticulum (ER) stress, the ER membrane protein IRE1 initiates the regulated splicing of Xbp1 mRNA, leading to the production of a potent transcription factor that helps cells restore proteostasis. We report that Xbp1 is a...
Taraxacum officinale Seed Extract Inhibits HeLa Cell Migration at Sub-cytotoxic Concentrations [0.03%]
蒲公英叶提取物通过降低人宫颈癌HeLa细胞的迁移能力抑制肿瘤进展
Christina Hendrickson,Melville B Vaughan,John Nail et al.
Christina Hendrickson et al.
Dandelion seed extract exhibits potent anticancer activity in HeLa cells. HeLa viability decreased with increasing doses of DSE from 0 to 0.8 mg/mL, while HDF viability was not affected under similar conditions. DSE reduced HeLa cells' migr...
Quantification of eef1a1l1 transcript in early zebrafish development [0.03%]
斑马鱼早期发育过程中eef1a1l1转录本的定量分析
Gloria D Ligunas,Stefan C Materna
Gloria D Ligunas
Eukaryotic elongation factor 1-alpha is among the most highly expressed genes in vertebrates. Because its transcript levels are less sensitive to perturbations than those of other housekeeping genes, the zebrafish ortholog eef1a1l1 is widel...
Exploring Proteomic Differences in PBMCs for Sex-Specific Insights into Alzheimer's Disease [0.03%]
探索阿尔茨海默病性别差异的PBMC蛋白组学研究
Takako Ishida-Takaku,Colin Combs
Takako Ishida-Takaku
Peripheral blood mononuclear cells (PBMCs) offer a minimally invasive window into systemic biology and immune dysregulation in Alzheimer's disease (AD). We performed quantitative proteomic profiling of PBMCs from male and female AD patients...
Neuropeptides Are Involved in Elicited Reversal Speed Plasticity in C. elegans During Mechanosensory Habituation [0.03%]
神经肽参与了秀丽隐杆线虫在机械感觉习惯化过程中诱发的逆转速度可塑性
Audrey Siu,Nikolas Kokan,Catharine H Rankin
Audrey Siu
Caenorhabditis elegans respond to mechanosensory taps with brief reversal responses. In past research, speed of the response was averaged over the entire reversal for each tap to analyze reversal speed habituation; however, with this measur...
Reversible Chemogenetic Fluorescence Labeling with pFAST in C. elegans [0.03%]
利用pFAST在秀丽隐杆线虫中实现可逆化学遗传荧光标记
Zixu Wang,Dominique Langevin,Vivian Chen et al.
Zixu Wang et al.
The promiscuous fluorescence-activating and absorption-shifting tag (pFAST) enables reversible chemogenetic labeling with multiple fluorogens. We generated a single-copy tandem pFAST (td-pFAST) transgenic Caenorhabditis elegans strain expre...
Mengyuan Yi,Hannah Sonnenberg,Vimala Anthonydhason et al.
Mengyuan Yi et al.
Precise detection of endogenous protein expression is essential for understanding gene function in vivo . We generated a Drosophila knock-in line inserting a 3×Ollas tag at the C-terminus of the endogenous biniou ( bin ) locus, enabling sp...
Isolation and genomic analysis of secondary metabolism in cave Actinomycetota from biofilms and Ceuthophilus [0.03%]
从生物膜和Ceuthophilus中分离洞穴放线菌并进行二级代谢物的基因组分析
Ilyana O Bachmann,Brian O Bachmann
Ilyana O Bachmann
We report the isolation and genomic sequencing of nine Actinomycetota obtained from both cave biofilms and trogloxenic Ceuthophilus (cave crickets). Strains were isolated from samples via actinomycetota-selective media and sequenced using n...
Structural Basis for how Sialoglycan-binding Viridans Streptococci Accommodate Ligands that Exceed the Characterized Binding Site [0.03%]
化糖结合的革兰氏阳性菌适应超出已知结合位点的配体的结构基础
KeAndreya M Morrison,Kole Martin,Hai Yu et al.
KeAndreya M Morrison et al.
During endocardial infections, viridans group streptococci use proteins containing siglec-like binding regions to engage sialic acid-capped O- GalNAc glycans on platelet glycoprotein GPIbα. Much past work used isolated di-, tri-, or tetras...
Deletion of Rspo1 or Rspo3 in the mesenchyme does not affect Wolffian duct maintenance or morphogenesis [0.03%]
基质中删除Rspo1或Rspo3不影响 wolffian 系统的维持或形态发生
Shuai Jia,Jillian Wilbourne,Allyssa Fogarty et al.
Shuai Jia et al.
Secreted proteins, R-Spondin 1 (RSPO1) and R-Spondin 3 (RSPO3), potentiate WNT/β-catenin signaling that play critical roles in reproductive organ development. However, the functional significance of RSPO1 and RSPO3 in Wolffian duct develop...