Long-read plasmid sequencing strategy for evaluating intrinsic instability of tandem gene arrays [0.03%]
评估串联基因阵列固有不稳定的长读质粒测序策略
Hiroaki Takesue,Satoshi Okada,Takashi Ito
Hiroaki Takesue
Tandem gene arrays are inherently unstable, particularly in recombination-prone organisms such as the budding yeast Saccharomyces cerevisiae . However, understanding the nature of this instability-how frequently and to what extent the targe...
The Schizosaccharomyces pombe nucleolar protein Nsk1 modulates rDNA silencing during interphase [0.03%]
模式生物裂殖酵母核糖体DNA异染色质化调控机制研究进展
Jun-Song Chen,Sarah M Hanna,Alaina H Willet et al.
Jun-Song Chen et al.
Schizosaccharomyces pombe Nsk1 acts at kinetochores during mitosis to prevent error-prone chromosome segregation and it is phosphoregulated by Cdk1 . The Clp1 / Cdc14 protein phosphatase, to which Nsk1 binds, reverses Cdk1-mediated phosphor...
Temporal induction of the homeodomain transcription factor Nkx2-1 is sufficient to respecify foregut and hindgut endoderm to a pulmonary fate in Xenopus laevis [0.03%]
非洲爪蟾中Nkx2-1同源域转录因子的时空调控足以将前肠和后肠内胚层细胞重编程为肺组织
Brian A Hyatt,Erin Lundberg,Rachael Eye et al.
Brian A Hyatt et al.
The ability of transcription factors (TFs) to regulate cell fate decisions is paramount in developmental, homeostatic, and pathogenic contexts. The homeodomain TF NKX2-1 is an essential and evolutionarily conserved master regulator of pulmo...
Pep12 is important for proteasome microautophagy under low glucose conditions [0.03%]
Pep12在低葡萄糖条件下对蛋白酶体微自噬作用很重要
Kyle VanderVen,Conner Butcher,Remi&#x; Fokine et al.
Kyle VanderVen et al.
Autophagic degradation of proteasomes is a highly conserved mechanism for regulating proteasome homeostasis in eukaryotes. Here we show that Pep12, a t-SNARE protein, is important for intralumenal vesicle formation in the vacuole and protea...
Skeletal muscle TFEB overexpression does not increase neurogenesis markers in the young female hippocampus [0.03%]
青年女性海马区骨骼肌TFEB过表达不会增加神经发生标志物
Mia Hakian,Ian Matthews,Constanza J Cortes
Mia Hakian
Adult hippocampal neurogenesis (AHN), the process in which new neurons are formed in the dentate gyrus of the hippocampus, declines with age and is highly responsive to voluntary wheel running in mice. This exercise-activated increase in AH...
Megan E Lawson,Kelsey Gammage,Calvin Dexel et al.
Megan E Lawson et al.
Gene model for the ortholog of Regulator of cullins 1a ( Roc1a ) in the May 2011 (Broad dper_caf1/DperCAF1) Genome Assembly (GenBank Accession: GCA_000005195.1 ) of Drosophila persimilis . This ortholog was characterized as part of a develo...
Hyperactivation of mTORC1 by an endogenous raga-1 gain-of-function mutation does not reduce lifespan in C. elegans [0.03%]
内源性RAGA-1增效突变通过过度激活mTORC1不对秀丽隐杆线虫寿命产生负面影响
Tatiana M Moreno,Michelle E Brown,Caroline Kumsta
Tatiana M Moreno
Inhibition of mTORC1, a conserved nutrient-sensing complex, extends lifespan across model organisms, but the effects of mTORC1 hyperactivation are less understood. RagA, a GTPase essential for mTORC1 activation, can be locked in its active ...
A small-scale bacterial-based liquid Culture Method for Steinernema hermaphroditum [0.03%]
一种用于Steinernema hermaphroditum的小型细菌基液体培养方法
Nathan Y Rodak,Chieh-Hsiang Tan,Paul W Sternberg
Nathan Y Rodak
Entomopathogenic nematodes (EPN) infect and kill their insect host with the help of symbiotic bacteria. The only known hermaphroditic (androdiecious) EPN, the clade IV Steinernema hermaphroditum , offers opportunities for exploring both par...
The underappreciated, underrecognized problem of fourth chromosome trisomy in Drosophila melanogaster stocks and a simple, general method for building diplo-4 stocks from triplo-4 stocks [0.03%]
果蝇品系中第四染色体三体型被低估和未被认识的问题及其从三倍体品系构建二倍体品系的简单通用方法
Kevin R Cook
Kevin R Cook
Trisomy of the small, fourth chromosome is common in Drosophila melanogaster stocks. Even though the presence of an extra chromosome can confound the interpretation of experimental crosses, many Drosophila geneticists are unaware of this po...
Characterization of temperature-sensitive alleles of the septation initiation network protein Mob1 in Schizosaccharomyces pombe [0.03%]
模式生物胎盘丝状真菌温度敏感型Septation Initiation Network (SEI)信号蛋白Mob1突变体的表征研究
Sarah M Hanna,Alaina H Willet,Kathleen L Gould
Sarah M Hanna
Schizosaccharomyces pombe Mob1 is the regulatory subunit of the protein kinase Sid2 . The Sid2- Mob1 complex is the most downstream acting component of the septation initiation network (SIN). In the absence of functional Mob1 , cells fail c...