Identification of a potential internalization or endocytic compartment-targeting signal in the C-terminal tail of the tetraspanin protein TSP-12 [0.03%]
鉴定四跨膜蛋白TSP-12C端尾部潜在的内吞或囊泡靶向信号
Zhiyu Liu,Byron Williams,Jun Liu
Zhiyu Liu
Tetraspanins are unique yet highly conserved four-pass transmembrane proteins that play important roles in diverse biological processes. The C. elegans tetraspanin protein TSP-12 is localized both at the cell surface and in multiple intrace...
A cross-species rescue by mating method to interrogate gene essentiality across the Saccharomyces genus [0.03%]
一种跨物种拯救新方法：通过交配方式在酿酒酵母属中鉴定必需基因
Taylor K Wang,Abigail Keller,Omar Kunjo et al.
Taylor K Wang et al.
The field of budding yeast research has long been empowered by the vast array of genetic tools and community resources available. Rescue by mating is one useful tool that entails mating of meiotic spores directly post-germination. This mini...
Suppression of the fat storage defect of C. elegans dbl-1/BMP signaling mutants correlates with improved survival on a bacterial pathogen [0.03%]
抑制C. elegans双信号突变体的脂肪存储缺陷与细菌病原菌上的生存改善相关
Katerina K Yamamoto,Alan Cantos,Daeun Grace Chun et al.
Katerina K Yamamoto et al.
Innate immunity is a fundamental form of defense that is regulated by conserved signaling pathways. One such pathway is the DBL-1 /BMP (Bone Morphogenetic Protein) pathway, which is known to have a role in the immune response in the nematod...
A practical guide to sample preparation for immunoprecipitation coupled with mass spectrometry analysis of C. elegans proteins [0.03%]
用于果蝇蛋白质谱分析的免疫沉淀样品制备实用指南
Jian-Hua Wang,Meng-Qiu Dong
Jian-Hua Wang
The success of i mmuno p recipitation coupled with m ass s pectrometry (IPMS) analysis is tied to the amount of sample input and the expression level of the bait protein in the sample. Here, we selected six C. elegans proteins- VIT-2 , LMN-...
Simultaneous and Sequential Coexposures of Serratia marcescens in Caenorhabditis elegans [0.03%]
Serratia marcescens 的同时和顺序共暴露对线虫Caenorhabditis elegans的影响
Emily B Smith,Jennifer D Gresham,Andrew Huang et al.
Emily B Smith et al.
Coinfections are common in nature and include hosts simultaneously or sequentially exposed to different genotypes. We investigated the potential for simultaneous and sequential exposures to impact host fitness by exposing Caenorhabditis ele...
The Effectiveness of Manuka Honey in Treating Staphylococcus aureus in C. elegans [0.03%]
麦卢卡蜂蜜处理万古霉素耐药表皮葡萄球菌和金黄色葡萄球菌感染的线虫的效果研究
Riley Lesko,Olivia Long,Kelsey Murphy
Riley Lesko
Antibiotic resistance is a severe problem stemming from the overuse of antibiotics. Manuka honey, a unique honey from New Zealand, may serve as an alternative to traditional antibiotics for treating bacterial infections. A transgenic line o...
Expression of a phage RecET recombinase system improves detection of shorter DNA fragments by an Acinetobacter baylyi ADP-ISx antibiotic gene biosensor strain [0.03%]
一短DNA片段表达的类芽孢杆菌ADP-ISx抗生素生物传感器菌株的噬菌体RecET重组酶系统表达改善了检测效果
Nicholas Rhyan,Issac S Koshy,Ryan Dinh et al.
Nicholas Rhyan et al.
Naturally competent bacteria have been engineered into sequence-specific biosensors for environmental DNA, but low recombination rates limit detection of small DNA fragments. Phage recombinases are often used to increase the efficiency of i...
Intestinal and epidermal-specific analysis of ZIP-1 function and expression in Caenorhabditis elegans [0.03%]
利用秀丽隐杆线虫进行ZIP-1功能和表达的肠道及表皮特异性分析
James A Tirtorahardjo,Vladimir Lažetić,Emily R Troemel
James A Tirtorahardjo
In the nematode Caenorhabditis elegans , infection with intracellular intestinal pathogens, including microsporidia and the Orsay virus, activates a transcriptional immune response called the intracellular pathogen response (IPR). Upregulat...
Cyst stem cell lineage GAL4 transgenes are robustly expressed in hub cells of the Drosophila testis stem cell niche [0.03%]
类固醇干细胞系GAL4转基因在果蝇睾丸干细胞区室的hub细胞中有效表达
Jennifer Viveiros,Erika Matunis
Jennifer Viveiros
Manipulating gene expression in the different cell types of the Drosophila melanogaster testis stem cell niche is critical to understanding its biology. Here, we tested the specificity of commonly used somatic cyst stem cell (CySC) lineage ...
Genome Sequence of the Gordonia rubripertincta Phage Yucky (Cluster CT) [0.03%]
红斑链球菌噬菌体Yucky（CT组）基因组序列
Caitlin Carter,Paxton R Evitts,Cheyenne Helton et al.
Caitlin Carter et al.
Novel bacteriophage Yucky infects Gordonia rubripertincta , a bacterium known for its bioremediation potential. Phage particles have a siphovirus morphology and form 1.2 mm ± 0.5 mm-wide plaques (n=10). The genome of Yucky is 47,803 bp in ...