har-1/CHCHD10 mutations induce neurodegeneration and mitochondrial fragmentation in Caenorhabditis elegans [0.03%]
har-1/CHCHD10突变通过线虫神经元模型诱导神经退行性疾病和线粒体碎裂效应
Audrey Labarre,Ericka Guitard,Gilles Tossing et al.
Audrey Labarre et al.
CHCHD10 encodes a mitochondrial protein that plays a role in cristae morphology and oxidative phosphorylation, with mutations associated with neurodegenerative diseases, including the spectrum of amyotrophic lateral sclerosis and frontotemp...
Suppression of har-1/CHCHD10 phenotypes for ALS-FTD therapy discovery [0.03%]
利用抑制har-1/CHCHD10表型发现ALS-FTD疗法
Audrey Labarre,Ericka Guitard,Gilles Tossing et al.
Audrey Labarre et al.
Mutations in CHCHD10 are linked to a variety of neurodegenerative diseases, including amyotrophic lateral sclerosis and frontotemporal dementia (ALS-FTD). The Caenorhabditis elegans orthologue of CHCHD10 is har-1 , and we investigated wheth...
A comparative analysis of distinct assays for the investigation of Caenorhabditis elegans behavior during organophosphate exposure [0.03%]
有机磷暴露下用不同的检测方法对线虫行为学进行分析的一个比较研究
Johanna Haszczyn,Vincent O&#x;Connor,Lindy Holden-Dye et al.
Johanna Haszczyn et al.
C. elegans motility is a convenient paradigm to describe the behavioral outcome of genetic- and drug-induced changes in neural circuits. Motility may be parameterized by scoring movement on solid medium or in liquid. In addition, body wall ...
Collaborative research efforts benefit both Primarily Undergraduate Institution faculty and students and a biotechnology company in reproducibility project [0.03%]
合作研究项目使文理学院师生和生物科技公司都受益于可重复性项目
Kristen C Johnson,Lori Hensley,Christin Pruett et al.
Kristen C Johnson et al.
Undergraduate students often have limited access to industry-focused research opportunities. To address this, faculty and students from 10 primarily undergraduate institutions collaborated with Sampling Human, a biotechnology company, to te...
Associative learning by C. elegans is variable when butanone is paired with starvation [0.03%]
四氢萘与饥饿感结合可使秀丽隐杆线虫的学习过程产生变化
Samiha Tasnim,Amber Liu,Antony Jose
Samiha Tasnim
The nematode C. elegans has been reported to show a reduction in its preference for the odorant butanone after prior exposure to butanone coupled with starvation. Here we report unexplained variability in such associative learning. Pre-expo...
Investigating the effects of liquid handling robot pipetting speed on yeast growth and gene expression using growth assays and RNA-seq [0.03%]
采用生长测定和RNA测序研究液体处理机器人移液速度对酵母生长和基因表达的影响
Shodai Taguchi,Ryosuke Matsuzawa,Yasuyuki Suda et al.
Shodai Taguchi et al.
Assessing the impact of experimental parameters like pipetting speed is essential in life science research but challenging in manual experiments. Recent advancements in laboratory automation enable precise control and systematic evaluation ...
Genomic Complexity of ccdc40 in Xenopus : Implications for CRISPR Targeting and Disease Modeling [0.03%]
非洲爪蟾ccdc40基因组复杂性研究及其在CRISPR靶向和疾病建模中的意义
Takuya Nakayama,Saurabh Kulkarni
Takuya Nakayama
Mutations in CCDC40 cause primary ciliary dyskinesia in humans. To evaluate the pathogenicity of variants in CCDC40 , we examined the genomic structure of this gene in Xenopus tropicalis , a diploid frog suitable as a model for genetic stud...
Microparticle Bombardment as a Method for Transgenesis in Auanema and Tokorhabditis [0.03%]
秀丽隐杆线虫和近缘种中的转基因新技术：显微注射微粒轰击法
Tatsuya Yamashita,Andre Pires-daSilva,Shun Oomura et al.
Tatsuya Yamashita et al.
Functional gene analysis tools in Caenorhabditis elegans are often ineffective in other nematodes due to differences in gonadal morphology and transgene silencing. Here, we established a method to generate stable transgenic lines in the nem...
Insertion of mNeonGreen into the variable domain of DRP-1 permits visualization of functional endogenous protein [0.03%]
将mNeonGreen插入DRP-1可变域使其能够在不干扰其功能的情况下被观察到
Eric J Lambie,Barbara Conradt
Eric J Lambie
We used CRISPR-Cas9 editing of the genomic drp-1 locus in C. elegans to test whether the mitochondrial fission function of DRP-1 was retained following insertion of mNeonGreen into the variable domain. We found that DRP-1 activity remains l...
UCK2-dependent conversion of cytidine to CTP is required for CTP uptake by Chlamydia trachomatis [0.03%]
UCK2介导的胞苷向CTP转化对沙眼衣原体摄取CTP是必需的
Laure Blanchet,Agathe Subtil
Laure Blanchet
Chlamydia trachomatis , an obligate intracellular bacterium, develops into a vacuolar compartment called the inclusion. The bacteria import nucleoside triphosphates (NTPs) present in the inclusion lumen. It remains unclear whether nucleosid...