53bp1 mutation enhances brca1 and bard1 embryonic lethality in C. elegans [0.03%]
53BP1突变增强秀丽隐杆线虫BRCA1和BARD1的胚胎致死性
Sara Hariri,Qianyan Li,JoAnne Engebrecht
Sara Hariri
In mice, mutation of brca1 results in embryonic lethality, which is partially suppressed by 53bp1 mutation. In contrast, mutation of the C. elegans BRCA1 ortholog, brc-1 , or its binding partner, brd-1 , lead to only mild embryonic lethalit...
SHE9 deletion mutants display fitness defects during diauxic shift in Saccharomyces cerevisiae [0.03%]
∆SHE9突变株在Saccharomyces cerevisiae中经历二次利用期时表现出适应性缺陷
Shane J Kowaleski,Christina S Hurmis,Carvin N Coleman et al.
Shane J Kowaleski et al.
Saccharomyces cerevisiae protein She9 is localized to the inner mitochondrial membrane and is required for normal mitochondrial morphology. While deletion mutants of SHE9 ( she9Δ ) are viable and display large ring-like mitochondrial struc...
Complete sequences of conjugal helper plasmids pRK2013 and pEVS104 [0.03%]
完整的接合辅助质粒pRK2013和pEVS104的序列信息
Ashley Hall,Timothy Donohue,Jason Peters
Ashley Hall
We present the complete sequences of two commonly used conjugal helper plasmids: pRK2013 and pEVS104. These sequences will enable engineering of custom helper plasmids, for example, with different antibiotic markers or origins of replicatio...
Prolongation of mitosis is associated with enhanced endogenous DNA damage in fission yeast [0.03%]
细胞内源性DNA损伤与裂殖酵母有丝分裂延长有关
Ishutesh Jain,Phong T Tran
Ishutesh Jain
Mitosis is usually shorter than other phases of the cell cycle and maintains a consistent duration despite variations in cell size and spindle size. This suggests the existence of a compensatory mechanism that ensures a short duration, poss...
A simulation test of the prediction that density-dependent dispersal promotes female-biased sex allocation in viscous populations [0.03%]
密度依赖扩散促进黏性种群中雌偏性性别分配的预测仿真测试
Chedhawat Chokechaipaisarn,Andy Gardner
Chedhawat Chokechaipaisarn
A classic result of sex-allocation theory is that the sex ratio is predicted to be invariant with respect to the rate of dispersal. However, a recent mathematical analysis has suggested that if individuals are able to adjust their probabili...
A missense mutation in the C. elegans src-2 tyrosine-protein kinase reduces brood size and enhances embryonic morphogenesis defects in src-1(RNAi) conditions [0.03%]
秀丽隐杆线虫src-2酪氨酸蛋白质激酶错义突变体的遗传学分析
Xiaofei Bai,Rebecca Green,Tao Cai et al.
Xiaofei Bai et al.
Goldenhar Syndrome is a rare congenital disorder characterized by hemifacial microsomia. Although select mutations have been mapped for this disorder, the genetic etiologies in the majority of cases remain unknown. A recent clinical report ...
Characterizing short germline-specific promoters with a range of expression levels in C. elegans [0.03%]
具有不同表达水平的短特化胚系启动子的特性研究——以秀丽隐杆线虫为例
Mohammed D Aljohani,Sonia El Mouridi,Christian Frøkjær-Jensen
Mohammed D Aljohani
A core tenet of synthetic biology is that well-characterized regulatory elements are essential for engineering biological systems. Here, we characterize the specificity and expression levels of 18 short (254 to 880 bp) candidate germline pr...
AraC-induced neuron-like differentiation of human NTERA2/D1 cells and quantification of endogenous pre-mir-106b and 19b levels [0.03%]
AraC诱导的人类NTERA2/D1细胞神经元样分化及内源性pre-mir-106b和19b水平的测定
Yuka Kaneko,Tomoko Takahashi
Yuka Kaneko
MicroRNAs (miRNAs) are approximately 22 nucleotide-long non-coding RNAs that are encoded in the genome. miRNAs form base pairs with target mRNAs in the RNA-induced silencing complex and repress their expression through a mechanism called RN...
Sensitive detection of lipopolysaccharides by monitoring of interleukin-10 secretion from human PBMCs [0.03%]
人PBMCs分泌IL-10监测灵敏测定脂多糖
Daniel Ivanusic,Joachim Denner
Daniel Ivanusic
Lipopolysaccharide (LPS) contaminations may falsify immunological experiments and are crucial for pharmaceutical products because they cause life-threatening immune reactions. Here, we present interleukin-10 (IL-10) as a reliable marker to ...
Replication stress by MMS stimulates DNA synthesis in post-replicative G2-phase in S. pombe [0.03%]
MMS诱发的复制压力可激活模式生物裂殖酵母G2期的DNA复制机制
Seong Min Kim,Susan L Forsburg
Seong Min Kim
DNA replication is generally limited to S-phase but replication stress can drive cells to undergo DNA synthesis outside of S-phase. Mitotic DNA synthesis pathway is known to be activated to deal with replication stress-induced chromosomal i...