Samirul Bashir,Debnath Pal,Ozaira Qadri et al.
Samirul Bashir et al.
IRE1 belongs to a type I transmembrane protein family harboring two functional domains, cytoplasmic domain with kinase and RNAse catalytic activity, and the luminal domain, which is involved in the sensing of unfolded proteins. IRE1 molecul...
The ATPase activity of the phosphatidylethanolamine flippase TAT-5 inhibits extracellular vesicle budding from the plasma membrane [0.03%]
磷脂乙醇胺翻转酶TAT-5的ATP酶活性可抑制从质膜出芽的细胞外囊泡的产生
Lauren R Pitts,Julia Frondoni,Alexander T Nguyen et al.
Lauren R Pitts et al.
Cells release extracellular vesicles (EVs) from their surface, but the mechanisms that govern EV release by plasma membrane budding are poorly understood. The lipid flippase TAT-5 inhibits EV release from the plasma membrane in C. elegans ,...
SAF-A/hnRNP U binds polyphosphoinositides via a lysine rich polybasic motif located in the SAP domain [0.03%]
SAF-A/hnRNP U通过SAP结构域中的丰富赖氨酸多碱性基序结合多磷酸肌醇类物质
Amanda J Edson,Rhîan G Jacobsen,Aurélia E Lewis
Amanda J Edson
Polyphosphoinositides (PPIn) play essential functions as lipid signalling molecules and many of their functions have been elucidated in the cytoplasm. However, PPIn are also intranuclear where they contribute to chromatin remodelling, trans...
JKK-1(3E), a JKK-1 mutant with predicted phosphomimetic amino acid substitutions [0.03%]
假定的模拟磷酸化氨基酸替代突变体JKK-1(3E)
Inka Busack,Henrik Bringmann
Inka Busack
Phosphomimetic substitutions have been instrumental in understanding the role of phosphorylation in protein function. Here we describe the design and construction of a predicted phosphomimetic allele of the JUN kinase kinase gene jkk-1 in C...
Endogenous gpdh-1 transcriptional reporters as new tools for the study of the osmotic stress response [0.03%]
渗透胁迫反应研究的新工具——内源性GPDH-1转录报告基因
María Victoria Veroli,Todd Lamitina
María Victoria Veroli
In vivo monitoring of gpdh-1 gene expression using standard transcriptional reporters is a powerful and commonly used tool for genetic dissection of the osmotic stress response in C. elegans . Like all transgene reporters, these gpdh-1 repo...
The actin bundling protein Fascin is important for proper early development in Strongylocentrotus purpuratus embryos [0.03%]
束状蛋白Fascin在海胆Spuerorum胚胎早期发育中具有重要作用
Michael D Testa,Carolyn M Remsburg,Jia L Song
Michael D Testa
Fascin is a conserved protein that has been shown to modulate the cytoskeleton. Its role in early development remains unclear. After fertilization, embryos undergo rapid cell divisions, requiring the precise regulation of cytoskeleton to se...
Developmental exposure to the pesticide malathion enhances expression of Prdm12, a regulator of nociceptor development, in Xenopus laevis [0.03%]
农药马拉硫磷暴露通过增强Xenopus laevis 基因Prdm12 的表达影响痛觉感受器的发育
Valeria Donoso,Jeremy Whitson,Barbara Lom
Valeria Donoso
The transcription factor Prdm12 exerts important influences on the development of nociceptors, peripheral touch and pain-sensing neurons, and has been implicated in human pain sensation disorders. We examined the consequences of exposing de...
Targeted DNA demethylation of the Arabidopsis genome using the SunTag-dCpf1-TET1cd system [0.03%]
利用SunTag-dCpf1-TET1cd系统在拟南芥基因组中实现靶向DNA去甲基化
Yunxi Zheng,Reqing He
Yunxi Zheng
DNA methylation is a stable and heritable epigenetic mark, and it plays an important role in regulation of gene expression and transposon silencing. Here we developed a CRISPR/dCpf1-based targeted demethylation system using the catalytic do...
Taylor Barwell,Sofia Geld,Laurent Seroude
Taylor Barwell
The manipulation of gene expression requires complete control of space, time and level of expression. In Drosophila , the UAS/GAL4 system has been instrumental in achieving spatial control, but the pursuit for the ideal system for temporal ...
Sequence analyses of a lipoprotein conserved with bacterial actins responsible for swimming motility of wall-less helical Spiroplasma [0.03%]
螺旋Spiroplasma游泳运动的无壁鞭毛蛋白序列分析及与细菌肌动蛋白的保守性研究
Daichi Takahashi,Makoto Miyata
Daichi Takahashi
Spiroplasma is a genus of pathogenic or commensal cell-wall-deficient helical bacterium. Spiroplasma -specific protein fibril and five classes of bacterial actins, MreB1-5, are involved in a helical ribbon structure responsible for helical-...