Genetic Mapping and Phenotypic Characterization of the Drosophila AIF e04281 Allele Reveals Mutant Clone Loss in Mosaic Eyes [0.03%]
基因图谱和表型特征的Drosophila AIF e04281等位基因揭示马赛克眼睛中的突变体丢失现象
Rachel Harris,Ishaan Prasad,Sarah E Weaver et al.
Rachel Harris et al.
As part of the Fly-CURE consortium, a mutant allele of Apoptosis inducing factor ( AIF ) was characterized using complementation mapping, genomic sequencing, and mosaic phenotypic analysis to investigate its role in cell growth control in D...
Systematic comparison of mStayGold and mGold2 variants for live imaging in zebrafish [0.03%]
mStayGold和mGold2变异株在斑马鱼活体成像中的系统比较研究
Aline Tschanz,David Q Matus,Ian Swinburne
Aline Tschanz
Fluorescent proteins (FPs) are essential tools for live imaging, with brightness and photostability being key parameters when selecting an FP for experiments. Recently developed variants of monomeric StayGold (mSG) and mGold2 promise improv...
The CCR4-NOT deadenylase complex mediates tubulin autoregulation via specific adapters CNOT10 and CNOT11 [0.03%]
CCR4-NOT去腺苷酸化复合物通过特异的衔接蛋白CNOT10和CNOT11介导微管自调节
Stephanie L Sarbanes,J Robert Hogg,Antonina Roll-Mecak
Stephanie L Sarbanes
Tubulin autoregulation maintains cellular microtubule homeostasis by triggering rapid degradation of tubulin mRNAs in response to an increase in soluble α- and β-tubulin levels. Through siRNA knock-down of several RNA decay pathways coupl...
Time-lapse imaging of C. elegans egg-laying with high spatiotemporal resolution [0.03%]
高时空分辨率的C. elegans产卵延时成像技术
Siddharthan Balachandar Thendral,Nicole Roos,Johnny Vertiz et al.
Siddharthan Balachandar Thendral et al.
Egg-laying behavior in C. elegans is driven by a simple neural circuit comprising motor neurons, vulva muscles, and neuroendocrine cells. Most protocols designed to capture this behavior rely on imaging freely moving animals at low magnific...
CeSTAAN: An atlas of C. elegans adult neurons for fast queries of single-nucleus RNA sequencing data [0.03%]
CeSTAAN：一个快速查询秀丽隐杆线虫单核RNA测序数据的成年神经元图集
Jonathan St Ange,Henry Langmack,Coleen T Murphy
Jonathan St Ange
RNA-sequencing provides rich transcriptomic data about cell types, and new methods allow characterization at the single-cell level. For neurons, single-nucleus RNA sequencing (snSeq) is the gold standard, as this technique retains neuron id...
Toxic effects of GAL80 and RNAi against fluorescent proteins on motor performance in Drosophila [0.03%]
GAL80和RNAi干扰荧光蛋白对果蝇运动行为的影响
Kathryn Mizzi,Francesca Grech,Sylvana Tabone et al.
Kathryn Mizzi et al.
Transgenic regulators and reporter controls such as GAL80 and RNA interference (RNAi) constructs targeting fluorescent proteins are invaluable tools in Drosophila research. During experiments designed to temporally regulate gene expression,...
Localizations of the septin Spn4 tagged with GFP and mEGFP in fission yeast [0.03%]
绿色荧光蛋白(GFP)和黄色荧光蛋白(mEGFP)标记的裂殖酵母.SEPTINSPN4定位图案
Jack R Gregory,Nicholas J Ricottilli,Jian-Qiu Wu
Jack R Gregory
Septins are cytoskeletal proteins crucial for cell division and many other processes. In fission yeast, septins localize to the division site during septum formation. Recently, we conducted a study that elucidates the localizations and func...
Genome Sequences of Alatato and Audell, two Phages that infect Arthrobacter globiformis and have Different Life Cycles [0.03%]
分别感染不动细菌的Alatato和Audell两种噬菌体基因组序列及其不同的生活史特征
Sarah E Robinson,Erin Stanford,Shilen Abraham et al.
Sarah E Robinson et al.
Based on gene content similarity, Alatato and Audell are grouped to phage clusters FB and FR, respectively. Alatato has a 39,780bp genome and 71 genes of which 34 have a predicted function. Alatato is predicted to be temperate based on the ...
Complete Genome Sequences of seven cluster DE1 phages isolated using Gordonia terrae [0.03%]
七种用戈登菌分离的cluster DE1噬菌体的完整基因组序列
Azzam Almutairi,Gabriella Marsala,Natalie Mershon et al.
Azzam Almutairi et al.
The phages Barsten, Kamashten, Kwobi, LilHam, SchottB, StorminNorm and Thing3 were isolated from soil samples collected around Columbus, Ohio, United States, using the host Gordonia terrae 3612. The seven genomes range in size between 55,59...
Isolation and Characterization of the subcluster L2 Mycobacteriophage Underpass and its lysogen [0.03%]
underpass噬菌体及其溶源菌的分离与表征
Kiley R Toth,Alexa R Rogers,Emma R Wenzel et al.
Kiley R Toth et al.
We report here on mycobacteriophage Underpass, isolated from a soil sample collected at a construction site at Saint Joseph's University in Philadelphia, Pennsylvania. Underpass is a siphovirus that infects Mycobacterium smegmatis mc 2 155,...