Stepwise progression of β-selection during T cell development involves histone deacetylation [0.03%]
组蛋白去乙酰化在T细胞发育过程中β-选择的逐步进展中的作用
Anchi S Chann,Mirren Charnley,Lucas M Newton et al.
Anchi S Chann et al.
During T cell development, the first step in creating a unique T cell receptor (TCR) is genetic recombination of the TCRβ chain. The quality of the new TCRβ is assessed at the β-selection checkpoint. Most cells fail this checkpoint and d...
A high-content endogenous GLUT4 trafficking assay reveals new aspects of adipocyte biology [0.03%]
高内涵内源性GLUT4转运分析揭示了新的脂肪细胞生物学特征
Alexis Diaz-Vegas,Dougall M Norris,Sigrid Jall-Rogg et al.
Alexis Diaz-Vegas et al.
Insulin-induced GLUT4 translocation to the plasma membrane in muscle and adipocytes is crucial for whole-body glucose homeostasis. Currently, GLUT4 trafficking assays rely on overexpression of tagged GLUT4. Here we describe a high-content i...
Profiling subcellular localization of nuclear-encoded mitochondrial gene products in zebrafish [0.03%]
斑马鱼核基因编码的线粒体蛋白亚细胞定位分析
Barbara Uszczynska-Ratajczak,Sreedevi Sugunan,Monika Kwiatkowska et al.
Barbara Uszczynska-Ratajczak et al.
Most mitochondrial proteins are encoded by nuclear genes, synthetized in the cytosol and targeted into the organelle. To characterize the spatial organization of mitochondrial gene products in zebrafish (Danio rerio), we sequenced RNA from ...
GhRabA4c coordinates cell elongation via regulating actin filament-dependent vesicle transport [0.03%]
GhRabA4c通过调节依赖于肌动蛋白丝的囊泡运输来协调细胞伸长
Xiaoguang Shang,Yujia Duan,Meiyue Zhao et al.
Xiaoguang Shang et al.
Plant cell expands via a tip growth or diffuse growth mode. In plants, RabA is the largest group of Rab GTPases that regulate vesicle trafficking. The functions of RabA protein in modulating polarized expansion in tip growth cells have been...
Dose-dependent phosphorylation and activation of Hh pathway transcription factors [0.03%]
Hedgehog信号转导途径转录因子的剂量依赖性的磷酸化和激活
Mengmeng Zhou,Yuhong Han,Bing Wang et al.
Mengmeng Zhou et al.
Graded Hedgehog (Hh) signaling is mediated by graded Cubitus interruptus (Ci)/Gli transcriptional activity, but how the Hh gradient is converted into the Ci/Gli activity gradient remains poorly understood. Here, we show that graded Hh induc...
Mechanistic and pharmacodynamic studies of DuoBody-CD3x5T4 in preclinical tumor models [0.03%]
双特异抗体DuoBody-CD3x5T4的机制和药代动力学研究
Kristel Kemper,Ellis Gielen,Peter Boross et al.
Kristel Kemper et al.
CD3 bispecific antibodies (bsAbs) show great promise as anticancer therapeutics. Here, we show in-depth mechanistic studies of a CD3 bsAb in solid cancer, using DuoBody-CD3x5T4. Cross-linking T cells with tumor cells expressing the oncofeta...
Mitochondrial stress-induced GFRAL signaling controls diurnal food intake and anxiety-like behavior [0.03%]
线粒体压力诱导的GFRAL信号调控昼夜进食行为和类似焦虑的行为
Carla Igual Gil,Bethany M Coull,Wenke Jonas et al.
Carla Igual Gil et al.
Growth differentiation factor 15 (GDF15) is a mitochondrial stress-induced cytokine that modulates energy balance in an endocrine manner. However, the importance of its brainstem-restricted receptor GDNF family receptor alpha-like (GFRAL) t...
HIV-1 infection enhances innate function and TLR7 expression in female plasmacytoid dendritic cells [0.03%]
HIV-1感染可增强女性浆细胞样树突状细胞的天然免疫功能及其TLR7表达
Flora Abbas,Claire Cenac,Ali Youness et al.
Flora Abbas et al.
Plasmacytoid dendritic cells (pDCs) express TLR7, a ssRNA-sensor encoded on the X chromosome, which escapes X chromosome inactivation (XCI) in females. pDCs are specialized in the production of type 1 interferons (IFN-I) through TLR7 activa...
Meiotic DNA breaks activate a streamlined phospho-signaling response that largely avoids protein-level changes [0.03%]
减数分裂DNA断裂激活了一种精简的磷酸化信号响应机制，很大程度上避免了蛋白质水平的变化
Funda M Kar,Christine Vogel,Andreas Hochwagen
Funda M Kar
Meiotic cells introduce a numerous programmed DNA breaks into their genome to stimulate meiotic recombination and ensure controlled chromosome inheritance and fertility. A checkpoint network involving key kinases and phosphatases coordinate...
The human RNA polymerase I structure reveals an HMG-like docking domain specific to metazoans [0.03%]
人RNA聚合酶I结构揭示了一种特有后生动物的HMG样对接域
Julia L Daiß,Michael Pilsl,Kristina Straub et al.
Julia L Daiß et al.
Transcription of the ribosomal RNA precursor by RNA polymerase (Pol) I is a major determinant of cellular growth, and dysregulation is observed in many cancer types. Here, we present the purification of human Pol I from cells carrying a gen...