GPER1 links estrogens to centrosome amplification and chromosomal instability in human colon cells [0.03%]
GPER1将雌激素与人类结肠细胞中的中心体放大和染色体不稳定联系起来
Miriam Bühler,Jeanine Fahrländer,Alexander Sauter et al.
Miriam Bühler et al.
The role of the alternate G protein-coupled estrogen receptor 1 (GPER1) in colorectal cancer (CRC) development and progression is unclear, not least because of conflicting clinical and experimental evidence for pro- and anti-tumorigenic act...
Endoplasmic reticulum membranes are continuously required to maintain mitotic spindle size and forces [0.03%]
内质网膜持续维持有丝分裂纺锤体大小和力量所需
Margarida Araújo,Alexandra Tavares,Diana V Vieira et al.
Margarida Araújo et al.
Membrane organelle function, localization, and proper partitioning upon cell division depend on interactions with the cytoskeleton. Whether membrane organelles also impact the function of cytoskeletal elements remains less clear. Here, we s...
Discovery of RUF6 ncRNA-interacting proteins involved in P. falciparum immune evasion [0.03%]
参与恶性疟原虫免疫逃避的RUF6 ncRNA互作蛋白的发现
Gretchen M Diffendall,Anna Barcons-Simon,Sebastian Baumgarten et al.
Gretchen M Diffendall et al.
Non-coding RNAs (ncRNAs) are emerging regulators of immune evasion and transmission of Plasmodium falciparum RUF6 is an ncRNA gene family that is transcribed by RNA polymerase III but actively regulates the Pol II-transcribed var virulence ...
Genome-wide profiling of histone modifications in Plasmodium falciparum using CUT&RUN [0.03%]
使用CUT&RUN进行恶性疟原虫的组蛋白修饰全基因组分析
Riward Campelo Morillo,Chantal T Harris,Kit Kennedy et al.
Riward Campelo Morillo et al.
We recently adapted a CUT&RUN protocol for genome-wide profiling of chromatin modifications in the human malaria parasite Plasmodium Using the step-by-step protocol described below, we were able to generate high-quality profiles of multiple...
LACTB exerts tumor suppressor properties in epithelial ovarian cancer through regulation of Slug [0.03%]
LACTB通过调节Slug在上皮性卵巢癌中发挥肿瘤抑制特性
Valentina Cutano,Jessica Marianne Ferreira Mendes,Sara Escudeiro-Lopes et al.
Valentina Cutano et al.
Epithelial-mesenchymal transition (EMT) is a cellular mechanism used by cancer cells to acquire migratory and stemness properties. In this study, we show, through in vitro, in vivo, and 3D culture experiments, that the mitochondrial protein...
Loss of autism-candidate CHD8 perturbs neural crest development and intestinal homeostatic balance [0.03%]
自闭症候选基因CHD8的丢失扰乱了神经嵴的发育和肠道稳态平衡
Gaëlle Hayot,Mathieu Massonot,Céline Keime et al.
Gaëlle Hayot et al.
Individuals with mutations in CHD8 present with gastrointestinal complaints, yet the underlying mechanisms are understudied. Here, using a stable constitutive chd8 mutant zebrafish model, we found that the loss of chd8 leads to a reduced nu...
SMA-linked SMN mutants prevent phase separation properties and SMN interactions with FMRP family members [0.03%]
与SMA连锁的SMN突变体阻止了液-液相分离属性以及SMN与FMRP家族成员的互作关系
Olivier Binda,Franceline Juillard,Julia Novion Ducassou et al.
Olivier Binda et al.
Although recent advances in gene therapy provide hope for spinal muscular atrophy (SMA) patients, the pathology remains the leading genetic cause of infant mortality. SMA is a monogenic pathology that originates from the loss of the SMN1 ge...
Lipid saturation induces degradation of squalene epoxidase for sterol homeostasis and cell survival [0.03%]
脂质饱和通过降解角鲨烯环氧酶来维持固醇的稳态和细胞存活
Leng-Jie Huang,Rey-Huei Chen
Leng-Jie Huang
A fluid membrane containing a mix of unsaturated and saturated lipids is essential for life. However, it is unclear how lipid saturation might affect lipid homeostasis, membrane-associated proteins, and membrane organelles. Here, we generat...
Protein and RNA ADP-ribosylation detection is influenced by sample preparation and reagents used [0.03%]
蛋白质和RNA的ADP核糖基化检测受样品制备和所用试剂的影响
Lisa Weixler,Nonso Josephat Ikenga,Jim Voorneveld et al.
Lisa Weixler et al.
The modification of substrates with ADP-ribose (ADPr) is important in, for example, antiviral immunity and cancer. Recently, several reagents were developed to detect ADP-ribosylation; however, it is unknown whether they recognise ADPr, spe...
Lack of peroxisomal catalase affects heat shock response in Caenorhabditis elegans [0.03%]
过氧化物体中的过氧化氢酶缺乏影响了秀丽隐杆线虫的热激反应
Marina Musa,Pedro A Dionisio,Ricardo Casqueiro et al.
Marina Musa et al.
Exact mechanisms of heat shock-induced lifespan extension, although documented across species, are still not well understood. Here, we show that fully functional peroxisomes, specifically peroxisomal catalase, are needed for the activation ...