An Optimized Workflow for In Vitro Transcription of Single Guide RNAs Minimizes Innate Immune Activation [0.03%]
优化体外转录单导向RNA的工作流程以最小化固有免疫激活作用
Xin Wang,Wim Trypsteen,Jasper Anckaert et al.
Xin Wang et al.
CRISPR interference (CRISPRi) often uses single guide RNAs (sgRNAs) generated by in vitro transcription (IVT); however, IVT-derived RNAs can trigger innate immune responses that confound functional analyses. Here, we evaluate innate immune ...
Rodolphe Barrangou
Rodolphe Barrangou
The Diversifying Distribution Trends of Maturing CRISPR Technologies by Addgene [0.03%]
Addgene公司成熟CRISPR技术多元化的分布趋势分析
Alyssa Shepard,Elena Minones-Moyano,Christina Mork et al.
Alyssa Shepard et al.
Since the advent of Cas9-based CRISPR technologies in 2012, there has been a remarkable growth in genome editing research, literature, applications, and translational impact. Much of this research has been fueled by the global dissemination...
Development and Characterization of RNA Aptamer-Mediated Modular Base Editors Containing Staphylococcus aureus Cas9 Derivatives and Novel Deaminase Orthologs [0.03%]
基于金黄色葡萄球菌Cas9衍生物和新型脱氨酶正交酶的RNA适配体介导模块化碱基编辑器的发展与表征
Juan Carlos Collantes,Kellen Xu,Melany Ruiz-Urigüen et al.
Juan Carlos Collantes et al.
Base editing enables precise genome modifications without introducing DNA double-strand breaks. Using Streptococcus pyogenes Cas9 as a prototype, we previously developed a modular base editing platform in which the deaminase is recruited by...
Evaluation of Prime Editing Efficiency in Human Immortalized MSC-TERT Cells with Osteogenic Potential for Modeling FGFR2-Linked Craniosynostosis [0.03%]
用于建模FGFR2相关颅缝早闭的成骨诱导的人永生间充质干细胞-MSC-TERT细胞素编辑效率评估研究
Max Gijsbertsen,Filipa M Duarte,Ana Fuentes Manjón et al.
Max Gijsbertsen et al.
Craniosynostosis is a rare congenital bone condition where skull sutures fuse prematurely and is linked to mutations in over 60 genes. Generating mutation-specific in vitro models allows investigation of craniosynostosis-associated mutation...
Induction of SpCas9-Directed Immune Responses Using Lipid Nanoparticles and Identification of SpCas9-Derived T Cell Epitopes in C57BL/6 Mice [0.03%]
使用脂质纳米颗粒诱导SpCas9引起的免疫反应并鉴定C57BL/6小鼠中SpCas9衍生的T细胞表位
Deja Porenta,Naomi Benne,Alice Sijts et al.
Deja Porenta et al.
A major goal of clinically oriented CRISPR-Cas9-based applications is safe and effective in vivo gene editing (knockout or correction) with precise targeting. Substantial efforts have been devoted to the preclinical development of novel dru...
Dorothy Semenow
Dorothy Semenow
Joshua B Black,Charles A Gersbach
Joshua B Black
Increasing the Effective Gene Drive Homing Rate by Targeting the Haploinsufficient Spermatogenesis Gene Klhl10 [0.03%]
通过靶向单倍体功能不全的 spermatogenesis 基因 Klhl10 来提高基因驱动同源重组率
Ceili L Peng,W Sebastian Kamau,Julien Freeman et al.
Ceili L Peng et al.
CRISPR-based gene drives represent a powerful new technology for limiting disease transmission and controlling invasive populations. These systems rely on homology-directed repair (HDR) to "drive" a genetic element through a population. How...