Maximizing light-driven CO2 and N2 fixation efficiency in quantum dot-bacteria hybrids [0.03%]
量子点-细菌杂化体系中光驱动CO2和N2固定的效率最大化研究
Xun Guan,Sevcan Erşan,Xiangchen Hu et al.
Xun Guan et al.
Integrating light-harvesting materials with microbial biochemistry is a viable approach to produce chemicals with high efficiency from the air, water, and sunlight. Yet it remains unclear whether all absorbed photons in the materials can be...
Principles of target DNA cleavage and the role of Mg2+ in the catalysis of CRISPR-Cas9 [0.03%]
CRISPR-Cas9靶DNA切割原则及Mg2+催化作用
Łukasz Nierzwicki,Kyle W East,Jonas M Binz et al.
Łukasz Nierzwicki et al.
At the core of the CRISPR-Cas9 genome-editing technology, the endonuclease Cas9 introduces site-specific breaks in DNA. However, precise mechanistic information to ameliorating Cas9 function is still missing. Here, multi-microsecond molecul...
Molecular mechanism of topoisomerase poisoning by the peptide antibiotic albicidin [0.03%]
アルバシ丁によるトポイソメラーゼ毒性作用の分子機構
Elizabeth Michalczyk,Kay Hommernick,Iraj Behroz et al.
Elizabeth Michalczyk et al.
The peptide antibiotic albicidin is a DNA topoisomerase inhibitor with low-nanomolar bactericidal activity towards fluoroquinolone-resistant Gram-negative pathogens. However, its mode of action is poorly understood. We determined a 2.6 Å r...
Bypassing evolutionary dead ends and switching the rate-limiting step of a human immunotherapeutic enzyme [0.03%]
绕过进化死胡同并切换人类免疫治疗酶的限速步骤
John Blazeck,Christos S Karamitros,Kyle Ford et al.
John Blazeck et al.
The Trp metabolite kynurenine (KYN) accumulates in numerous solid tumours and mediates potent immunosuppression. Bacterial kynureninases (KYNases), which preferentially degrade kynurenine, can relieve immunosuppression in multiple cancer mo...
Evidence of substrate binding and product release via belt-sulfur mobilization of the nitrogenase cofactor [0.03%]
氮固定酶辅因子通过带状硫的移动来结合底物和释放产物的证据
Chi Chung Lee,Wonchull Kang,Andrew J Jasniewski et al.
Chi Chung Lee et al.
The Mo-nitrogenase catalyses the ambient reduction of N2 to NH3 at the M-cluster, a complex cofactor that comprises two metal-sulphur partial cubanes ligated by an interstitial carbide and three belt-sulphurs. A recent crystallographic stud...
Olefin Functionalization/Isomerization Enables Stereoselective Alkene Synthesis [0.03%]
烯烃的官能团化/异构化实现立体选择性合成 алкenes
Chen-Fei Liu,Hongyu Wang,Robert T Martin et al.
Chen-Fei Liu et al.
Despite tremendous efforts aimed at devising methods for stereoselective alkene synthesis, critical challenges are yet to be addressed. Direct access to a diverse range of 1aryl(boryl)-1-methyl-functionalized tri- and tetrasubstituted trans...
Biocatalytic synthesis of non-standard amino acids by a decarboxylative aldol reaction [0.03%]
脱羧型烯醇化反应催化的非标准氨基酸生物合成反应
Jonathan M Ellis,Meghan E Campbell,Prasanth Kumar et al.
Jonathan M Ellis et al.
Enzymes are renowned for their catalytic efficiency and selectivity. Despite the wealth of carbon-carbon bond forming transformations in traditional organic chemistry and nature, relatively few C-C bond forming enzymes have found their way ...
Elucidation of radical- and oxygenate-driven paths in zeolite-catalysed conversion of methanol and methyl chloride to hydrocarbons [0.03%]
甲醇和氯化甲酯催化解析产生自由基和氧合物驱动途径的阐明
Alessia Cesarini,Sharon Mitchell,Guido Zichittella et al.
Alessia Cesarini et al.
Understanding hydrocarbon generation in the zeolite-catalysed conversions of methanol and methyl chloride requires advanced spectroscopic approaches to distinguish the complex mechanisms governing C-C bond formation, chain growth and the de...
Catalytic mechanism and endo-to- exo selectivity reversion of an octalin-forming natural Diels-Alderase [0.03%]
八氢苊形成型天然茋二烯酶的催化机制及其内-外选择性转换研究
Michio Sato,Shinji Kishimoto,Mamoru Yokoyama et al.
Michio Sato et al.
We have previously reported the identification of CghA, a proposed Diels-Alderase responsible for the formation of the bicyclic octalin core of the fungal secondary metabolite Sch210972. Here we show the crystal structure of the CghA-produc...
Deacylation-Aided C─H Alkylative Annulation through C─C Cleavage of Unstrained Ketones [0.03%]
通过C─C键断裂的未张紧酮类的脱酰基作用辅助的C─H烷基化环加成反应
Xukai Zhou,Yan Xu,Guangbin Dong
Xukai Zhou
Arene- and heteroarene-fused rings are pervasive in biologically active molecules. Direct annulation between a C─H bond on the aromatic core and a tethered alkyl moiety provides a straightforward approach to access these scaffolds; however...