Development of a human antibody that exhibits antagonistic activity toward CC chemokine receptor 7 [0.03%]
一种具有拮抗CC趋化因子受体7活性的抗体的人源化发展研究
Moon-Sung Jang,Nurain Syahirah Binti Ismail,Yeon Gyu Yu
Moon-Sung Jang
Background: CC chemokine receptor 7 (CCR7) is a member of G-protein-coupled receptor family and mediates chemotactic migration of immune cells and different cancer cells induced via chemokine (C-C motif) ligand 19 (CCL19)...
Improving antibody thermostability based on statistical analysis of sequence and structural consensus data [0.03%]
基于序列和结构一致性数据分析改进抗体的热稳定性
Lei Jia,Mani Jain,Yaxiong Sun
Lei Jia
Background: The use of Monoclonal Antibodies (MAbs) as therapeutics has been increasing over the past 30 years due to their high specificity and strong affinity toward the target. One of the major challenges toward their ...
Adenovirus-vectored SARS-CoV-2 vaccine expressing S1-N fusion protein [0.03%]
表达S1-N融合蛋白的腺病毒载体新型冠状病毒(SARS-CoV-2)疫苗
Muhammad S Khan,Eun Kim,Alex McPherson et al.
Muhammad S Khan et al.
Additional COVID-19 vaccines that are safe and immunogenic are needed for global vaccine equity. Here, we developed a recombinant type 5 adenovirus vector encoding for the SARS-CoV-2 S1 subunit antigen and nucleocapsid as a fusion protein (...
Intracellular flow cytometry staining of antibody-secreting cells using phycoerythrin-conjugated antibodies: pitfalls and solutions [0.03%]
使用藻红蛋白偶联抗体对浆细胞进行细胞内流式细胞术染色：陷阱与解决方案
Patrick Renner,Michael Crone,Matthew Kornas et al.
Patrick Renner et al.
Background: Antibody-secreting cells are terminally differentiated B cells that play a critical role in humoral immunity through immunoglobulin secretion along with possessing the potential to be long-lived. It is now app...
Strategies to mitigate the on- and off-target toxicities of recombinant immunotoxins: an antibody engineering perspective [0.03%]
重组免疫毒素的脱靶毒性的策略：抗体工程技术观点
Mengyu Li,Sen Mei,Yi Yang et al.
Mengyu Li et al.
Targeted cancer therapies using immunotoxins have achieved remarkable efficacy in hematological malignancies. However, the clinical development of immunotoxins is also faced with many challenges like anti-drug antibodies and dose-limiting t...
Development of a T cell-redirecting bispecific antibody targeting B-cell maturation antigen for the suppression of multiple myeloma cell growth [0.03%]
靶向B细胞成熟抗原的T细胞重定向双特异性抗体抑制多发性骨髓瘤细胞增殖的发展
Jianxin Huo,Yuhan Huang,Ziying Zheng et al.
Jianxin Huo et al.
Background: Multiple myeloma (MM) is the second most common hematological malignancy. It has emerged as one of the next possible hematological diseases amenable to immunotherapy. B-cell maturation antigen (BCMA), a member...
Massively multiplexed affinity characterization of therapeutic antibodies against SARS-CoV-2 variants [0.03%]
针对SARS-CoV-2变异毒株的治疗性抗体的大规模多重亲和力表征
Emily Engelhart,Randolph Lopez,Ryan Emerson et al.
Emily Engelhart et al.
Antibody therapies represent a valuable tool to reduce COVID-19 deaths and hospitalizations. Multiple antibody candidates have been granted emergency use authorization by the Food and Drug Administration and many more are in clinical trials...
Evaluation of site-specific methylation of the CMV promoter and its role in CHO cell productivity of a recombinant monoclonal antibody [0.03%]
CMV启动子特异性甲基化对其在CHO细胞中表达重组单克隆抗体产力作用的评估
Hussain Dahodwala,Sophia D Amenyah,Sarah Nicoletti et al.
Hussain Dahodwala et al.
We previously demonstrated that increased monoclonal antibody productivity in dihydrofolate reductase (DHFR)-amplified CHO cells correlates with phosphorylated transcription factor-cytomegalovirus (CMV) promoter interactions. In this articl...
Productivity and quality improvement for a symmetric bispecific antibody through the application of intensified perfusion cell culture [0.03%]
基于增强灌流培养技术的对称双特异性抗体产率和质量优化
Yongjun Qin,Rongmei Ma,Yang Li et al.
Yongjun Qin et al.
Background: Aggregation, fragmentation, and low yield are issues frequently found during the cell culture process of bispecific antibodies (bsAbs), whose inherent complexity likely plays a role in causing these issues. ...
Assessment of chimeric antigen receptor T cytotoxicity by droplet microfluidics in vitro [0.03%]
用于评估嵌合抗原受体T细胞体外毒性的滴液式微流控体系
Kuan Un Wong,Jingxuan Shi,Peng Li et al.
Kuan Un Wong et al.
Chimeric antigen receptor T (CAR-T) cells are cytotoxic T cells engineered to specifically kill cancer cells expressing specific target receptor(s). Prior CAR-T efficacy tests include CAR expression analysis by qPCR or ELISA, in vitro measu...