On-column thiol protection strategy to control charge and stability of ThiomAb [0.03%]
柱上硫醇保护策略控制ThiomAb电荷和稳定性的研究
Kai Ni,Leo Lei Wang,Alan Shupe et al.
Kai Ni et al.
Background: Cysteine-engineered monoclonal antibodies (mAbs), or ThiomAb, enable site-specific conjugation and form the basis of several next-generation antibody-drug conjugates (ADCs). However, the presence of intentiona...
Integrating fab phage and yeast display through seamless transfer of paired heavy- and light-chain libraries for efficient antibody discovery [0.03%]
通过无缝传输配对的重链和轻链文库整合丝岛噬菌体与酵母展示用于高效的抗体发现
Wei Zhang,Hao Ye,Xingrun Zhang et al.
Wei Zhang et al.
Background: In vitro display technologies, especially phage and yeast display, are revolutionary tools in antibody discovery, enabling high-throughput screening and engineering. Previous efforts to integrate phage and yea...
Antibody-oligonucleotide conjugates: an emerging modality for precision RNA therapeutics [0.03%]
抗体寡核苷酸共轭物：面向精准RNA治疗的新兴药物模态
Chen-Hsu Yu,Summer Y Y Ha,Zhiqiang An et al.
Chen-Hsu Yu et al.
RNA-targeting therapeutics have enormous potential to precisely target disease-causing RNAs, extending beyond the traditional limits of "druggability" for small molecules, antibodies, and protein-targeting cell therapies. However, one cruci...
Novel ADC and γδ T cell engager targeting CDH17 for the therapy of gastrointestinal cancers [0.03%]
靶向CDH17的新型抗体药物偶联物和γδT细胞衔接器用于治疗消化道肿瘤
Mingcan Yu,Guangmao Mu,Peng Chen et al.
Mingcan Yu et al.
Background: Cadherin-17 (CDH17) is a cell-adhesion molecule physiologically expressed along the intestinal epithelial tight junctions. Aberrant overexpression of CDH17 in gastrointestinal (GI) cancers promotes tumor growt...
TROP2 antibody-drug conjugate: unique epitope engagement drives differentiated efficacy [0.03%]
TROP2抗体药物偶联剂：独特的靶点识别位点驱动了差异化的疗效
Hang Chen,Xiaoshan Min,Qingwen Cheng et al.
Hang Chen et al.
Background: Trophoblast cell surface antigen 2 (TROP2) is frequently overexpressed in various cancers and is associated with poor prognosis. While TROP2-targeted antibody-drug conjugates (ADCs), such as sacituzumab govite...
The evolving landscape of enzymatic technologies for precision synthesis of antibody-drug conjugates [0.03%]
抗体偶联药物精准合成的酶技术的发展格局
Thuc Oanh Hoang,Arshad J Ansari,Yong Zhang
Thuc Oanh Hoang
Precise control over how and where small-molecule drugs are covalently attached to monoclonal antibodies is increasingly vital for creating consistently efficacious and safe antibody-drug conjugates (ADCs) as powerful targeted therapies. En...
Cytotoxic activity and safety characterization of IMV-M™, a MUC16 × DR5 bispecific antibody [0.03%]
一种MUC16×DR5双特异性抗体IMV-M™的细胞毒活性及安全性研究
Victor S Goldmacher,Iosif M Gershteyn
Victor S Goldmacher
Background: IMV-M is a MUC16 × DR5 bispecific antibody that has demonstrated MUC16-selective antitumor activity. However, it remained unclear whether multisite binding to MUC16 is required for cytotoxicity, and whether c...
Preventing physical and chemical degradation of the LABL-Fc-MOGR5, a bifunctional peptide inhibitor, with formulation development approaches [0.03%]
通过制剂开发策略防止LABL-Fc-MOGR5双功能肽抑制剂的物理化学降解
Lun Xin,Rucha Mahadik,Monika Prorok et al.
Lun Xin et al.
Background: LABL-Fc-MOGR5 is a bifunctional peptide inhibitor designed to modulate immune responses in multiple sclerosis, including primary progressive multiple sclerosis. Although therapeutically promising, its developm...
Systematic functional screening of immunoreceptor tyrosine-based inhibitory motif domains identifies potent inhibitory modules for chimeric antigen receptor-T [0.03%]
基于免疫受体酪氨酸抑制基序的功能筛选鉴定出用于CAR-T的强力抑制模块
Ting Yang,Min Gao,Yufang Sun et al.
Ting Yang et al.
Background: Chimeric antigen receptor (CAR) T-cell therapy has revolutionized the treatment of hematologic malignancies but remains limited by on-target, off-tumor toxicity and the lack of precise mechanisms to restrain a...
Application of a novel LC-MS-based proteomic workflow for host cell protein monitoring and risk control in biotherapeutics [0.03%]
一种新型LC-MS基蛋白质组学工作流程在生物治疗药物宿主细胞蛋白监测和风险管理中的应用
Xiaojuan Yu,Jianhui Cheng,Qianchuan Lv et al.
Xiaojuan Yu et al.
Background: Comprehensive monitoring of host cell proteins (HCPs) is essential for ensuring the quality, safety, and consistency of antibody therapeutics. Conventional enzyme-linked immunosorbent assay-based assays provid...