Engineering antibody and protein therapeutics to cross the blood-brain barrier [0.03%]
工程改造抗体和蛋白质药物以穿越血脑屏障
Peng Zhao,Ningyan Zhang,Zhiqiang An
Peng Zhao
Diseases in the central nervous system (CNS) are often difficult to treat. Antibody- and protein-based therapeutics hold huge promises in CNS disease treatment. However, proteins are restricted from entering the CNS by the blood-brain barri...
Vector design for enhancing expression level and assembly of knob-into-hole based FabscFv-Fc bispecific antibodies in CHO cells [0.03%]
优化表达水平和基于柄-孔FabscFv-Fc双特异性抗体组装的向量设计在CHO细胞中的应用
Han Kee Ong,Ngan T B Nguyen,Jiawu Bi et al.
Han Kee Ong et al.
Background: Two-armed FabscFv-Fc is a favoured bispecific antibody (BsAb) format due to its advantages of the conventional IgG structure. Production of FabscFv-Fc requires expression of three polypeptide chains, one light...
Role of antibody engineering in generation of derivatives starting from MOv19 MAb: 40 years of biological/therapeutic tools against folate receptor alfa [0.03%]
抗体工程在从MOv19单克隆抗体生成衍生物中的作用：针对叶酸受体阿尔法的40年生物/治疗工具
Barbara Frigerio,Matilde Montermini,Canevari Silvana et al.
Barbara Frigerio et al.
In the 1980s, we developed and characterized numerous murine monoclonal antibodies (MAbs) directed against human tumor-associated antigens. This mini review is focused on the generation of derivatives of an anti-folate receptor α (FRα) MA...
The emergence of AntibodyPlus: the future trend of antibody-based therapeutics [0.03%]
抗体药物的发展趋势：从单克隆抗体到双特异性抗体及其后的时代
Yong Zhu,Shawn Shouye Wang,Zhaohui Sunny Zhou et al.
Yong Zhu et al.
To date, close to 100 canonical monoclonal antibody drugs have been approved by the FDA; furthermore, a number of antibody-derived therapeutics in nontraditional formats have reached late development stages and the market, and many more are...
Role of harvest depth filtration in controlling product-related impurities for a bispecific antibody [0.03%]
用于双特异性抗体控制产品质量相关杂质的收获深度过滤的作用
Ehsan Espah Borujeni,Weixin Jin,Chun Shao et al.
Ehsan Espah Borujeni et al.
Background: Bispecific antibodies (BsAb) belong to a novel antibody category with advantages over traditional mono-specific therapeutic antibodies. However, product variants are also commonly seen during the production of...
Discovery and multi-parametric optimization of a high-affinity antibody against interleukin-25 with neutralizing activity in a mouse model of skin inflammation [0.03%]
一种高亲和力的抗IL-25中和抗体的发现及其在小鼠皮肤炎症模型中的多参数优化
Ruth Bone,Brian J Fennell,Amy Tam et al.
Ruth Bone et al.
Background: Interleukin (IL)25 has been implicated in tissue homeostasis at barrier surfaces and the initiation of type two inflammatory signaling in response to infection and cell injury across multiple organs. We sought...
Xiaochen Lyu,Qichao Zhao,Julia Hui et al.
Xiaochen Lyu et al.
Antibody therapies have become an important class of therapeutics in recent years as they have exhibited outstanding efficacy and safety in the treatment of several major diseases including cancers, immune-related diseases, infectious disea...
Pan-cancer distribution of cleaved cell-surface amphiregulin, the target of the GMF-1A3 antibody drug conjugate [0.03%]
GMF-1A3抗体偶联药物的靶点裂解型细胞表面amphiregulin在多种癌症中的分布特征
Kristopher A Lofgren,Nicolette C Reker,Sreeja Sreekumar et al.
Kristopher A Lofgren et al.
Amphiregulin is a transmembrane protein which, when cleaved by the TACE/ADAM17 protease, releases a soluble epidermal growth factor receptor ligand domain that promotes proliferation of normal and malignant cells. We previously described a ...
A novel IgG Fc by computer-aided design enhances heavy-chain heterodimerization in bi- or trispecific antibodies [0.03%]
一种计算机辅助设计的IgG Fc增强了双特异性和多特异性抗体中的重链半胱氨酸介导的异二聚化
Bo Wang,Jun Lin,Matthew R Hoag et al.
Bo Wang et al.
The classical `knob-into-holes' (KIH) strategy (knob(T366Y)/hole (Y407T)) has successfully enhanced the heterodimerization of a bispecific antibody (BsAb) resulting in heterodimer formation up to 92% of protein A (ProA)-purified protein poo...
Use of Debye-Hückel-Henry charge measurements in early antibody development elucidates effects of non-specific association [0.03%]
德拜-休克尔-亨利电荷测定在早期抗体开发中的应用阐明了非特异性结合的影响
Joshua R Laber,Thomas M Laue,Dana I Filoti
Joshua R Laber
The diffusion interaction parameter (kD ) has been demonstrated to be a high-throughput technique for characterizing interactions between proteins in solution. kD reflects both attractive and repulsive interactions, including long-ranged el...