Addressing present pitfalls in 3D printing for tissue engineering to enhance future potential [0.03%]
解决组织工程中3D打印的当前缺陷以增强未来的潜力
Jesse K Placone,Bhushan Mahadik,John P Fisher
Jesse K Placone
Additive manufacturing in tissue engineering has significantly advanced in acceptance and use to address complex problems. However, there are still limitations to the technologies used and potential challenges that need to be addressed by t...
Quantitative evaluation of the impact of artificial cell adhesion via DNA hybridization on E-cadherin-mediated cell adhesion [0.03%]
人工DNA杂交粘附对E-钙黏着蛋白介导的细胞粘附的影响评估
Shodai Togo,Ken Sato,Ryuzo Kawamura et al.
Shodai Togo et al.
Programmable cell adhesion with DNA hybridization is a promising approach for fabricating various tissue architectures without sophisticated instrumentation. However, little is known about how this artificial interaction influences the bind...
Site-directed targeting of transcriptional activation-associated proteins to repressed chromatin restores CRISPR activity [0.03%]
将转录激活相关蛋白靶向压缩染色质以恢复CRISPR活性
René Daer,Fatima Hamna,Cassandra M Barrett et al.
René Daer et al.
Previously, we used an inducible, transgenic polycomb chromatin system to demonstrate that closed, transcriptionally silenced chromatin reduces Cas9 editing. Here, we investigated strategies to enhance Cas9 editing efficiency by artificiall...
An inexpensive programmable optogenetic platform for controlled neuronal activation regimens in C. elegans [0.03%]
一种用于控制秀丽隐杆线虫神经元激活模式的廉价光遗传学平台
Zachary Crawford,Adriana San-Miguel
Zachary Crawford
In Caenorhabditis elegans, optogenetic stimulation has been widely used to assess neuronal function, control animal movement, or assay circuit responses to controlled stimuli. Most studies are performed on single animals and require high-en...
Gregory H Underhill,Salman R Khetani
Gregory H Underhill
The liver executes 500+ functions, such as protein synthesis, xenobiotic metabolism, bile production, and metabolism of carbohydrates/fats/proteins. Such functions can be severely degraded by drug-induced liver injury, nonalcoholic fatty li...
Mechanical changes of peripheral nerve tissue microenvironment and their structural basis during development [0.03%]
发育过程中外周神经组织微环境的力学变化及其结构基础
Gonzalo Rosso,Jochen Guck
Gonzalo Rosso
Peripheral nerves are constantly exposed to mechanical stresses associated with body growth and limb movements. Although some aspects of these nerves' biomechanical properties are known, the link between nerve biomechanics and tissue micros...
Dario Collia,Luigino Zovatto,Gianni Pedrizzetti
Dario Collia
The clinical syndrome of mitral insufficiency is a common consequence of mitral valve (MV) prolapse, when the MV leaflets do not seal the closed orifice and blood regurgitates back to the atrium during ventricular contraction. There are dif...
Jian Zhou,Prithviraj Mukherjee,Hua Gao et al.
Jian Zhou et al.
Massive growth of the microfluidics field has triggered numerous advances in focusing, separating, ordering, concentrating, and mixing of microparticles. Microfluidic systems capable of performing these functions are rapidly finding applica...
AAV-mediated gene therapy targeting TRPV4 mechanotransduction for inhibition of pulmonary vascular leakage [0.03%]
利用AAV介导的基因疗法靶向TRPV4机械感受功能以抑制肺血管渗漏
Juan Li,Amy M Wen,Ratnakar Potla et al.
Juan Li et al.
Enhanced vascular permeability in the lungs can lead to pulmonary edema, impaired gas exchange, and ultimately respiratory failure. While oxygen delivery, mechanical ventilation, and pressure-reducing medications help alleviate these sympto...
A microfluidic platform for simultaneous quantification of oxygen-dependent viscosity and shear thinning in sickle cell blood [0.03%]
一种用于同时量化镰状细胞血红蛋白氧气依赖性粘度和剪切稀化的微流控平台
José M Valdez,Yvonne H Datta,John M Higgins et al.
José M Valdez et al.
The pathology of sickle cell disease begins with the polymerization of intracellular hemoglobin under low oxygen tension, which leads to increased blood effective viscosity and vaso-occlusion. However, it has remained unclear how single-cel...