Streamlining Cellular Thermal Shift assay for ultra-High Throughput Screening [0.03%]
高通量筛选的细胞热位移 assay 的简易化
Dr Pascal Lambertz,Loretta Hamacher,Dr Jana Flegel et al.
Dr Pascal Lambertz et al.
The Cellular Thermal Shift Assay (CETSA) has emerged as a powerful tool for evaluating drug-target interactions in live cells, yet its application in ultra-high throughput screening (uHTS) has been limited by technical constraints. In this ...
An ELISA for discovering protein-protein interaction inhibitors: blocking lysinoalanine crosslinking between subunits of the spirochete flagellar hook as a test case [0.03%]
一种用于发现蛋白质-蛋白质相互作用抑制剂的酶联免疫吸附测定法：螺旋体鞭毛钩亚基间赖氨素-鸟氨酸交联作用作为测试案例
Maithili Deshpande,Michael J Lynch,Kurni Kurniyati et al.
Maithili Deshpande et al.
The inhibition of a specific protein-protein interaction is often difficult to achieve in targeted drug design. We report the development and optimization of a general-purpose, readily implemented enzyme-linked immunosorbent assay (ELISA) f...
MICRO-TAG enzyme complementation enables quantification of cellular drug-target engagement in temperature series [0.03%]
基于温度系列的细胞内药物靶点结合程度的量化检测技术(MICRO-TAGE互补系统)
Ivan Babic,Nikolas Bryan,Claire Cunningham et al.
Ivan Babic et al.
Drug discovery for challenging drug targets necessitates the proteomic complexities of the cellular milieu for contextual target folding and function. Conventional biophysical methods for assessing drug interaction with a target are often n...
Fluorescent Probe-Based Detection of Outer Membrane Damage of Gram-negative Bacteria [0.03%]
基于荧光探针的革兰氏阴性菌外膜损伤检测方法
Bingchen Li,Qionglu Duan,Wenjing Shi et al.
Bingchen Li et al.
Infections caused by drug-resistant bacteria, particularly Gram-negative species, represent one of the most significant global public health challenges. The outer membrane (OM) is a crucial target for the development of drugs against Gram-n...
High-Throughput Screening Identifies Non-nucleoside Inhibitors of the SARS-CoV-2 Polymerase with Novel Mechanisms [0.03%]
高通量筛选发现SARS-CoV-2聚合酶非核苷抑制剂及其新机制
Colin R Woodford,Kristine E Frank,Haizhong Zhu et al.
Colin R Woodford et al.
The RNA-dependent RNA polymerase (RdRp) of coronaviruses, comprising highly conserved non-structural proteins, is a critical player in the viral lifecycle and represents a promising target for developing pan-coronavirus antivirals. Despite ...
PCIM: Learning Pixel Attributions via Pixel-Wise Channel Isolation Mixing in High Content Imaging [0.03%]
基于像素级通道隔离混合的学习高内涵成像中的像素归属方法
Daniel Siegismund,Mario Wieser,Stephan Heyse et al.
Daniel Siegismund et al.
Deep Neural Networks (DNNs) have shown remarkable success in various computer vision tasks. However, their black-box nature often leads to difficulty in interpreting their decisions, creating an unfilled need for methods to explain the deci...
Protocols and Research Articles in 3D Biology: Technologies and Methodologies Reshaping 3D Cell Culture [0.03%]
三维生物学中的协议和研究文章：重塑三维细胞培养的技术和方法学
Glauco R Souza,Evan Cromwell,Madhu Nag
Glauco R Souza
High-content immunofluorescence assay detecting PD-L1 expression changes in head and neck cancer patient-derived cultures [0.03%]
高内涵免疫荧光测定法检测头颈癌患者来源类器官中PD-L1表达的变化
Yuen-Keng Ng,Stacy Magdalene Abbang,Jishi Ye et al.
Yuen-Keng Ng et al.
Clinical uses of monoclonal antibodies against immune checkpoint molecules, such as the Program Death-Ligand 1 (PD-L1) or Program Death Protein-1 (PD-1), has transformed cancer therapy across pan-cancers. In addition to antibody therapies, ...
High-throughput combination screening of Pidnarulex and other G-quadruplex ligands in multi-cell type tumor spheroids [0.03%]
基于多细胞类型肿瘤类器官的Pidnarulex等G-四链体配体高通量组合筛选研究
Thomas S Dexheimer,Nathan P Coussens,Thomas Silvers et al.
Thomas S Dexheimer et al.
G-quadruplexes (G4s) are four-stranded nucleic acid structures that regulate key cellular processes and represent promising therapeutic targets in oncology. To investigate the therapeutic potential of three G4 ligands-pidnarulex, APTO-253, ...
Comparative analysis of antibody-mediated loss-of-function versus gene knock-out and knock-down [0.03%]
抗体介导的功能丧失与基因敲除和干扰的比较分析
Marie Buck-Wiese,Sally Liechocki,Holger Erfle et al.
Marie Buck-Wiese et al.
In this study we compare three methods for manipulating cell function: RNA interference (RNAi), CRISPR-Cas9 gene knock-out, and antibody-mediated loss-of-function. We have focused on analyzing changes in cell-matrix adhesion via targeting t...