VOC analysis for rapid, early detection of bacteria, mold, and mycoplasma in cell and tissue cultures [0.03%]
用于快速检测细胞和组织培养中细菌、霉菌和支原体的挥发性有机物分析
Hannah A Strobel,Manohar P Bhandari,Eva Borras et al.
Hannah A Strobel et al.
Identifying microbial contamination early in cell culture processing can save time, money, and reagents. However, standard strategies for performing contamination testing often take days, leaving production vulnerable to potential spread of...
Temperature-related intensity change (TRIC)-based high throughput screening platform for the discovery of CHI3L1-targeted small molecules [0.03%]
基于温度相关强度变化（TRIC）的高通量筛选平台发现CHI3L1靶向小分子
Longfei Zhang,Moustafa T Gabr
Longfei Zhang
Chitinase-3-like 1 (CHI3L1) protein is a secreted glycoprotein involved in various normal physiological processes, while its abnormal elevation is closely associated to carcinogenesis. CHI3L1 recruits and polarizes immune cells into the tum...
Pawel Leznicki,Karl Deacon,Bohdan Waszkowycz et al.
Pawel Leznicki et al.
Here, we review general trends in biological and small molecule cytokine drug discovery, highlighting key learnings from two successful case studies (TNFα and IL-17), which have helped shape our understanding of how best to identify new sm...
The NLRP3 signaling pathway is a potential target for clinical translation in glioma treatment [0.03%]
NLRP3信号通路可能是胶质瘤治疗中具有临床转化潜力的作用靶点
Fengyu Cheng,Yuanyuan Ming,Yunsong Pan et al.
Fengyu Cheng et al.
Glioma, particularly glioblastoma (GBM), stands as the most prevalent and formidable tumor in neurosurgery, marked by relatively poor prognosis and high recurrence rates. Despite significant advancements in multimodal therapies-encompassing...
Discovery and validation of small molecule stabilizers of mutant triose phosphate isomerase (TPI) as potential lead candidates for TPI Deficiency [0.03%]
三磷酸甘油醛异构酶(TPI)缺乏症小分子稳定剂的发现及验证
Laura L Vollmer,Presley Roberts,Samantha L Eicher et al.
Laura L Vollmer et al.
Triosephosphate Isomerase deficiency (TPI-Df) is a devastating untreatable childhood metabolic disease resulting in anemia, severe locomotor impairment, and premature death. Numerous single amino acid substitutions in TPI are pathogenic and...
Integrating AUROC and SSMD for Quality Control in High-Throughput Screening Assays [0.03%]
基于AUROC和SSMD的质量控制的高通量筛选试验集成方法研究
Xiaohua Douglas Zhang
Xiaohua Douglas Zhang
High-throughput screening (HTS) assays are pivotal in modern biomedical research, particularly in drug discovery and functional genomics. Ensuring the quality and reliability of HTS data is critical, especially when dealing with the small s...
Suganya Sivagurunathan,Patrick Byrne,Alán F Muñoz et al.
Suganya Sivagurunathan et al.
Background: Cell Painting, the leading image-based profiling assay, involves staining plated cells with six dyes that mark the different compartments in a cell. Such profiles can then be used to discover connections betwe...
Haiyan Xu,Rachel L Palte,Meredith M Rickard et al.
Haiyan Xu et al.
DNA repair is a critical component for the maintenance of genomic stability and cancer prevention. Werner syndrome helicase (WRN), a RecQ family helicase involved in DNA double-strand break (DSB) repair, has been identified as a promising t...
Jinhee Kim,Kwang-Eun Choi,Yuno Lee et al.
Jinhee Kim et al.
Cytotoxicity profiling of screening libraries is a critical component in early-stage drug discovery to identify compounds with undesirable toxic effects. Here, we report the cytotoxicity profiling of the Korea Chemical Bank (KCB) diversity ...
Development of novel high-throughput biochemical competition assays to identify ligands of human asialoglycoprotein receptor 1 [0.03%]
用于鉴定人肝asl糖蛋白受体1配基的新型高通量生化竞争性测定法的发展
Jianming Liu,Bradley Peter,Lauren Rhodes et al.
Jianming Liu et al.
Hepatocyte-specific Asialoglycoprotein receptor (ASGPR) and its native ligand N-acetylgalactosamine (GalNAc) have been actively exploited for targeted delivery of therapeutic and diagnostic agents to the liver. Identification of new potent ...